Recombinant Human MAD2L1-binding protein (MAD2L1BP)

Code CSB-YP624009HU
MSDS
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Source Yeast
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Code CSB-EP624009HU
MSDS
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Source E.coli
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Code CSB-EP624009HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP624009HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP624009HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MAD2L1BP
Uniprot No.
Alternative Names
Caught by MAD2 protein; CMT2; dJ261G23.1 ; KIAA0110; MAD2L1 binding protein; MAD2L1 binding protein isoform 2; MAD2L1-binding protein; Mad2l1bp; MD2BP_HUMAN; MGC11282; OTTHUMP00000016496; RP1 261G23.6
Species
Homo sapiens (Human)
Expression Region
1-274
Target Protein Sequence
MAAPEAEVLS SAAVPDLEWY EKSEETHASQ IELLETSSTQ EPLNASEAFC PRDCMVPVVF PGPVSQEGCC QFTCELLKHI MYQRQQLPLP YEQLKHFYRK PSPQAEEMLK KKPRATTEVS SRKCQQALAE LESVLSHLED FFARTLVPRV LILLGGNALS PKEFYELDLS LLAPYSVDQS LSTAACLRRL FRAIFMADAF SELQAPPLMG TVVMAQGHRN CGEDWFRPKL NYRVPSRGHK LTVTLSCGRP SIRTTAWEDY IWFQAPVTFK GFRE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.
Gene References into Functions
  1. MAD2-p31(comet) axis may serve as a potential therapeutic target for glioma. PMID: 29408509
  2. p31comet-induced cell death is mediated by interactions with Mad2 PMID: 26544187
  3. joint action of TRIP13 and p31(comet) also promotes the release of Mad2 from MCC, participates in the complete disassembly of MCC and abrogates checkpoint inhibition of APC/C PMID: 25092294
  4. Altered p31(Comet):Mad2 expression ratios may provide new insight into altered spindle assembly checkpoint function and the generation of chromosomal instability in tumors. PMID: 24131926
  5. attenuating the affinity of p31(Comet) for Mad2 by phosphorylation promotes SAC activity in mitosis. Specifically, phosphorylation of Ser-102 weakens p31(Comet)-Mad2 binding and enhances p31(Comet)-mediated bypass of the SAC. PMID: 24596092
  6. study found p31(comet) traffics on and off kinetochores and also present in the cytosol; Cells depleted of p31(comet) arrest in metaphase with mature bipolar kinetochore-microtubule attachments; propose that p31(comet) is an essential component of machinery that silences the checkpoint during each cell cycle PMID: 21965286
  7. the importance of p31(comet) in checkpoint silencing and its potential as a target for antimitotic therapies. PMID: 22544748
  8. The binding of p31(comet) to Mad2 in mitotic checkpoint complex may trigger a conformational change in Cdc20 that facilitates its phosphorylation by Cdk, and that the latter process may promote its dissociation from BubR1. PMID: 22566641
  9. both p31(comet) and ubiquitination of Cdc20 are critical mechanisms of checkpoint inactivation. They act redundantly to promote Mad2 dissociation from Cdc20 PMID: 21937719
  10. p31(comet) negatively regulates the spindle assembly checkpoint by extracting Mad2 from the MCC. PMID: 22100920
  11. This review presents knowledge of CMT2 and possible pathogenetic mechanisms responsible for the disease PMID: 22235654
  12. p31comet localizes to kinetochores in a C-Mad2-dependent manner. the Mad1:C-Mad2 complex undergoes regulation by p31comet-dependent 'capping'. PMID: 21772247
  13. Although p31(comet) binds to Mad2, it promotes the dissociation of Cdc20 from BubR1 in MCC. PMID: 21300909
  14. p31(comet, formerly known as Cmt2) counteracts the function of Mad2 and is required for the silencing of the spindle checkpoint PMID: 15257285
  15. p31(comet) does not have an impact on the formation of aneuploidy and tetraploidy found in human hepatocellular carcinoma. PMID: 17934339
  16. Data suggest that p31(comet)(CMT2) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2." PMID: 18022368
  17. p31(comet) induces tumor cell senescence by mediating p21(Waf1/Cip1) accumulation and Mad2 disruption and that these effects are dependent on a direct interaction of p31(comet) with Mad2. PMID: 19276188

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Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, spindle. Note=During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.
Protein Families
MAD2L1BP family
Database Links

HGNC: 21059

KEGG: hsa:9587

STRING: 9606.ENSP00000410818

UniGene: Hs.122346

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