MAD2L1BP Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA155738A0HU
Size	US$210
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Image	IHC image of CSB-RA155738A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA155738A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA155738A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing A431 cells stained with CSB-RA155738A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

Q15013

Target Names

MAD2L1BP

Alternative Names

Caught by MAD2 protein antibody; CMT2 antibody; dJ261G23.1 antibody; KIAA0110 antibody; MAD2L1 binding protein antibody; MAD2L1 binding protein isoform 2 antibody; MAD2L1-binding protein antibody; Mad2l1bp antibody; MD2BP_HUMAN antibody; MGC11282 antibody; OTTHUMP00000016496 antibody; RP1 261G23.6 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human MAD2L1BP protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

18A12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MAD2L1BP, also known as p31comet, plays a critical role in mitotic checkpoint regulation by binding to MAD2L1 and promoting the disassembly of the mitotic checkpoint complex. This interaction is essential for proper chromosome segregation and timely anaphase onset, making MAD2L1BP a key target for researchers investigating cell cycle control, spindle assembly checkpoint dynamics, and the molecular mechanisms underlying chromosomal instability in cancer.

This recombinant monoclonal antibody, generated in rabbit host against a synthetic peptide derived from human MAD2L1BP, offers the consistency and reliability that demanding cell biology research requires. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring reproducible results across extended studies and between laboratories.

The antibody has been validated across multiple detection platforms, providing flexibility for diverse experimental workflows. Immunohistochemistry performance has been demonstrated in paraffin-embedded human kidney tissue and human cervical cancer sections using citrate buffer antigen retrieval, with recommended dilutions of 1:50–1:200. For cellular localization studies, immunofluorescence staining has been successfully performed in HeLa cells, revealing specific signal patterns when counter-stained with DAPI. Flow cytometry validation in A431 cells confirms utility for quantitative single-cell analysis, with clear separation between specific staining and isotype control populations.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this antibody arrives ready for immediate use in your research. Whether you are investigating mitotic checkpoint signaling, exploring cell division mechanisms, or characterizing checkpoint dysfunction in disease models, this antibody provides a dependable tool for advancing your understanding of MAD2L1BP biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.

Gene References into Functions

MAD2-p31(comet) axis may serve as a potential therapeutic target for glioma. PMID: 29408509
p31comet-induced cell death is mediated by interactions with Mad2 PMID: 26544187
joint action of TRIP13 and p31(comet) also promotes the release of Mad2 from MCC, participates in the complete disassembly of MCC and abrogates checkpoint inhibition of APC/C PMID: 25092294
Altered p31(Comet):Mad2 expression ratios may provide new insight into altered spindle assembly checkpoint function and the generation of chromosomal instability in tumors. PMID: 24131926
attenuating the affinity of p31(Comet) for Mad2 by phosphorylation promotes SAC activity in mitosis. Specifically, phosphorylation of Ser-102 weakens p31(Comet)-Mad2 binding and enhances p31(Comet)-mediated bypass of the SAC. PMID: 24596092
study found p31(comet) traffics on and off kinetochores and also present in the cytosol; Cells depleted of p31(comet) arrest in metaphase with mature bipolar kinetochore-microtubule attachments; propose that p31(comet) is an essential component of machinery that silences the checkpoint during each cell cycle PMID: 21965286
the importance of p31(comet) in checkpoint silencing and its potential as a target for antimitotic therapies. PMID: 22544748
The binding of p31(comet) to Mad2 in mitotic checkpoint complex may trigger a conformational change in Cdc20 that facilitates its phosphorylation by Cdk, and that the latter process may promote its dissociation from BubR1. PMID: 22566641
both p31(comet) and ubiquitination of Cdc20 are critical mechanisms of checkpoint inactivation. They act redundantly to promote Mad2 dissociation from Cdc20 PMID: 21937719
p31(comet) negatively regulates the spindle assembly checkpoint by extracting Mad2 from the MCC. PMID: 22100920
This review presents knowledge of CMT2 and possible pathogenetic mechanisms responsible for the disease PMID: 22235654
p31comet localizes to kinetochores in a C-Mad2-dependent manner. the Mad1:C-Mad2 complex undergoes regulation by p31comet-dependent 'capping'. PMID: 21772247
Although p31(comet) binds to Mad2, it promotes the dissociation of Cdc20 from BubR1 in MCC. PMID: 21300909
p31(comet, formerly known as Cmt2) counteracts the function of Mad2 and is required for the silencing of the spindle checkpoint PMID: 15257285
p31(comet) does not have an impact on the formation of aneuploidy and tetraploidy found in human hepatocellular carcinoma. PMID: 17934339
Data suggest that p31(comet)(CMT2) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2." PMID: 18022368
p31(comet) induces tumor cell senescence by mediating p21(Waf1/Cip1) accumulation and Mad2 disruption and that these effects are dependent on a direct interaction of p31(comet) with Mad2. PMID: 19276188

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton, spindle. Note=During early mitosis, unevenly distributed throughout the nucleoplasm. From metaphase to anaphase, concentrated on the spindle.

Protein Families

MAD2L1BP family

Database Links

HGNC: 21059

KEGG: hsa:9587

STRING: 9606.ENSP00000410818

UniGene: Hs.122346

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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