Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA.Immobilized Human MICB at 2 μg/mL can bind Anti-MICA/B recombinant antibody(CSB-RA56059MA1HU). The EC50 is 0.9180-1.062 ng/mL.

Target Names

MICB

Uniprot No.

AAB42011.1

Alternative Names

/

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

23-309aa

Target Protein Sequence

AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAEDVLGAETWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYNGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSGKALVLQSQRTD

Mol. Weight

34.1 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

MICB functions as a stress-induced ligand for the activating receptor NKG2D on natural killer cells and CD8+ T cells, making it a critical node in immune surveillance and tumor immunology. This mammalian-expressed protein spanning residues 23–309 captures the complete extracellular domain architecture required for physiologically relevant NKG2D engagement, and its binding to an anti-MICA/B recombinant antibody yields an EC50 of 0.9180–1.062 ng/mL in functional ELISA, demonstrating the conformational integrity necessary for receptor-ligand interaction studies and therapeutic antibody epitope mapping. The quantified binding kinetics support its use in competitive inhibition assays to screen blocking antibodies, in SPR or BLI experiments for affinity characterization of NKG2D-targeting biologics, and as a positive control in immune checkpoint research focused on NK cell activation pathways. Purity exceeding 95% by SDS-PAGE and endotoxin below 1.0 EU/μg align with standards expected in cell-based functional assays where low contaminant levels are essential.

Code	CSB-MP5608HU
Abbreviation	Recombinant Human MICB protein, partial (Active)
MSDS	MSDS Datasheet
Size	$190
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA.Immobilized Human MICB at 2 μg/ml can bind Anti-MICA/B recombinant antibody(CSB-RA56059MA1HU). The EC₅₀ is 0.9180-1.062 ng/mL. Biological Activity Assay
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Recombinant Human MHC class I molecule (MICB), partial (Active)

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