Recombinant Human Membrane-associated phosphatidylinositol transfer protein 1 (PITPNM1), partial

Code CSB-YP018038HU
MSDS
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Source Yeast
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Code CSB-EP018038HU
MSDS
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Source E.coli
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Code CSB-EP018038HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018038HU
MSDS
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Source Baculovirus
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Code CSB-MP018038HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PITPNM1
Uniprot No.
Alternative Names
DRES9; Drosophila retinal degeneration B; Drosophila retinal degeneration B homolog; FLJ44997; membrane-associated 1; Membrane-associated phosphatidylinositol transfer protein 1; NIR 2; NIR-2; NIR2; OTTHUMP00000236414; OTTHUMP00000236415; Phosphatidylinositol transfer protein; phosphatidylinositol transfer protein membrane associated; PITM1_HUMAN; PITPnm 1; PITPNM; Pitpnm1; PYK2 N terminal domain interacting receptor 2 ; Pyk2 N-terminal domain-interacting receptor 2; RD 9; RD9; RDGB; RDGB1; RDGBA1; retinal degeneration B alpha 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the transfer of phosphatidylinositol (PI) between membranes. Binds PI, phosphatidylcholine (PC) and phosphatidic acid (PA) with the binding affinity order of PI > PA > PC. Regulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions.
Gene References into Functions
  1. results suggest that Nir2 not only enhances EMT in vitro and breast cancer metastasis in animal models, but also contributes to breast cancer progression in human patients. PMID: 25179602
  2. Phosphatidic acid production triggers Phosphatidylinositol 4,5-Bisphosphate replenishment mediated by Nir2 and Nir3 at endoplasmic reticulum-plasma membrane junctions. PMID: 25887399
  3. Nir2 translocates from the Golgi complex to the plasma membrane in response to GF stimulation. PMID: 23897088
  4. Results suggest a feedback mechanism that replenishes PM PIP2 during receptor-induced Ca(2+) signaling via the Ca(2+) effector E-Syt1 and the PITP Nir2 at ER-PM junctions. PMID: 24183667
  5. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis. PMID: 12077336
  6. A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking & distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration. PMID: 12225667
  7. Phosphorylation of Nir2 by Cdk1 facilitates its dissociation from the Golgi apparatus, and phospho-Nir2 is localized in the cleavage furrow and midbody during cytokinesis. PMID: 15125835
  8. Nir2 is involved in maintaining a critical DAG pool in the Golgi apparatus by regulating its consumption via the CDP-choline pathway PMID: 15723057
  9. RdgB homologs play a preemptive role in excluding endogenous and exogenous modified purine deoxyribonucleoside triphosphates (dTNPs) from incorporation into DNA. PMID: 17090528

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Subcellular Location
Cytoplasm. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Lipid droplet. Cleavage furrow. Midbody.
Protein Families
PtdIns transfer protein family, PI transfer class IIA subfamily
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 9003

OMIM: 608794

KEGG: hsa:9600

STRING: 9606.ENSP00000348772

UniGene: Hs.372295

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