Recombinant Human Mucosal addressin cell adhesion molecule 1 (MADCAM1), partial

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Code CSB-MP618776HU
Size $590
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP618776HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) MADCAM1.
  • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP618776HU could indicate that this peptide derived from Mammalian Cell-expressed Homo sapiens (Human) MADCAM1.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
MADCAM1
Uniprot No.
Research Area
Immunology
Alternative Names
Addressin mucosal; hMAdCAM 1 ; hMAdCAM-1; MACAM1; MADCA_HUMAN; MAdCAM 1; MAdCAM-1; Madcam1; Mucosal addressin cell adhesion molecule 1; Mucosal vascular addressin cell adhesion molecule 1
Species
Homo sapiens (Human)
Source
Mammalian cell
Expression Region
19-317aa
Target Protein Sequence
QSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGPTQGEVIPTGSSKPAGDQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
34.9 kDa
Protein Length
Extracellular Domain
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Gene References into Functions
  1. Both the ACT-1 assay and the MAdCAM-1 assay demonstrated acceptable reproducibility and repeatability. The assays were sufficiently stable to allow for clinical use. During clinical testing the assays demonstrated that vedolizumab was able to saturate peripheral cells at all doses tested. PMID: 25908521
  2. Doxo-induced apoptosis has a role in inhibiting hepatocellular carcinoma by targeting Madcam1 and AKT and blocking protein translation initiation PMID: 26124182
  3. MADCAM1 is up-regulated in liver cirrhosis with expression on peribiliary vascular plexus and lymphoid aggregates PMID: 23839340
  4. Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop. PMID: 23297416
  5. Findings suggest that the inactive alpha4beta7 and alpha4beta7 activated by different stimuli have distinct conformations with different structural requirements for MAdCAM-1 binding. PMID: 21296888
  6. Ca2+ is essential for cell tethering in flow and rolling interactions through alpha 4 integrins with MAdCAM-1 as substrate; Mg2+ promotes firm adhesion of cells on MAdCAM-1 but with much lower tethering efficiency in shear flow. PMID: 11705388
  7. A crystal structure for the two extracellular amino-terminal domains of human MAdCAM-1 PMID: 11807247
  8. more extensive expression of MAdCAM-1 in Crohn's disease (CrD), than in ulcerative colitis, which could contribute not only to mucosal inflammation, but also to transmural inflammation in CrD. PMID: 12100519
  9. MAdCAM-1/integrin alpha4beta7 homing system may participate in gastric inflammation in response to H pylori-infection and contributes to MALT formation, typically leading to the development of nodular gastritis. PMID: 14669317
  10. a marker of graft rejection in small intestine transplantation PMID: 15050155
  11. PI3-K/Akt is involved in the gut-specific differentiation of human intestinal microvascular endothelial cells, which results in expression of the mucosal addressin MAdCAM-1 PMID: 15483224
  12. Immobilized CCL25 and CCL28 are both able to trigger integrin alpha(4)beta(7)-dependent lymphocyte arrest on MAdCAM-1 under shear flow. PMID: 18308860
  13. mucosal addressin cell adhesion molecule 1 may represent an important determinant for the generation of mucosal damage in celiac disease PMID: 19157500
  14. MADCAM1 gene polymorphisms may be associated with the risk of chronic GVHD and may, also, affect mortality related to aHSCT. PMID: 19286444

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ov
Database Links

HGNC: 6765

OMIM: 102670

KEGG: hsa:8174

STRING: 9606.ENSP00000215637

UniGene: Hs.102598

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