Recombinant Human Myomesin-1 (MYOM1), partial

Code CSB-YP015361HU
MSDS
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Source Yeast
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Code CSB-EP015361HU
MSDS
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Source E.coli
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Code CSB-EP015361HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015361HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP015361HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYOM1
Uniprot No.
Alternative Names
190 kDa connectin-associated protein; 190 kDa titin-associated protein; EH myomesin; MGC134946; MGC134947; MYOM1; MYOM1_HUMAN; Myomesin (M protein) 1 (190kD); Myomesin 1 (skelemin) 185kDa; Myomesin 1 185kDa; Myomesin 1, 185kDa; Myomesin family member 1; Myomesin-1; Skelemin
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.
Gene References into Functions
  1. Crystal structure of the obscurin(-like-1):myomesin complex reveals a trans-complementation mechanism whereby an incomplete immunoglobulin-like domain assimilates an isoform-specific myomesin interdomain sequence. PMID: 27989621
  2. individual subfragments of titin and myomesin composed of Fn type III and Ig-like domains can activate expression of two IGF-1 splice forms in cultured myoblasts PMID: 25152160
  3. myomesin could act as a highly elastic ribbon to maintain the overall structural organization of the sarcomeric M-band. PMID: 22347812
  4. Our results suggest that the downregulation of MBNL proteins should lead to the abnormal splicing of MYOM1 exon 17a in DM1 muscle. PMID: 21794030
  5. EH-myomesin levels were up-regulated at least 10 times in dilated cardiomyopathy patients compared to controls. PMID: 21069531
  6. This missense mutation caused significant changes of biochemical and biophysical properties of myomesin fragments indicating a role in the pathogenesis of hypertrophic cardiomyopathy . PMID: 21256114
  7. Results identify muscle-type creatine kinase as a binding partner of a central portion of myomesin and the closely related M-protein. PMID: 12972258
  8. visco-elastic properties of myomesin might be crucial for the stability of the sarcomere PMID: 15890201

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Subcellular Location
Cytoplasm, myofibril, sarcomere, M line.
Database Links

HGNC: 7613

OMIM: 603508

KEGG: hsa:8736

STRING: 9606.ENSP00000348821

UniGene: Hs.464469

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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