Recombinant Human Myotubularin-related protein 4 (MTMR4), partial

Code CSB-YP889148HU
MSDS
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Source Yeast
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Code CSB-EP889148HU
MSDS
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Source E.coli
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Code CSB-EP889148HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP889148HU
MSDS
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Source Baculovirus
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Code CSB-MP889148HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MTMR4
Uniprot No.
Alternative Names
FYVE domain containing dual specificity protein phosphatase 2; FYVE domain-containing dual specificity protein phosphatase 2; FYVE domain-containing dual specificity protein phosphatase FYVE DSP2; FYVE-DSP2; Mtmr4; MTMR4_HUMAN; Myotubularin related protein 4; Myotubularin-related protein 4; ZFYVE11 ; zinc finger FYVE domain containing 11; Zinc finger FYVE domain containing protein 11; Zinc finger FYVE domain-containing protein 11
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Dephosphorylates proteins phosphorylated on Ser, Thr, and Tyr residues and low molecular weight phosphatase substrate para-nitrophenylphosphate. Phosphorylates phosphatidylinositol 3,4,5-trisphosphate (PIP3).
Gene References into Functions
  1. Here the authors demonstrate that green fluorescent protein-tagged MTMR4 is recruited to the Salmonella-containing vacuole and infection of cells depleted of endogenous MTMR4 results in a decrease in viable intracellular Salmonella. PMID: 27625994
  2. the PH-GRAM domain of human MTMR4 was cloned, overexpressed in Escherichia coli, purified and crystallized by the vapour-diffusion method PMID: 25195910
  3. MTMR4 is a necessary negative modulator for the homeostasis of BMP/Dpp signaling PMID: 23150675
  4. MTMR4 localizes at the interface of early and recycling endosomes to regulate trafficking. PMID: 20736309
  5. MTMR4 belongs to the myotubularin family of phosphoinositides phosphatases PMID: 9736772
  6. MTMR4 plays an important role in preventing the overactivation of TGFbeta signaling by dephosphorylating activated R-Smads that have been trafficked to early endosomes PMID: 20061380
  7. Data found that hMTMR4 (human MTMR4) and Nedd4 co-immunoprecipitated and co-localized to late endosomes. PMID: 19125695

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Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein. Note=Localized to perinuclear region.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class myotubularin subfamily
Tissue Specificity
Expressed in brain, heart, kidney, spleen, liver, colon, testis, muscle, placenta, thyroid gland, pancreas, ovary, prostate, skin, peripheral blood, and bone marrow.
Database Links

HGNC: 7452

OMIM: 603559

KEGG: hsa:9110

STRING: 9606.ENSP00000325285

UniGene: Hs.514373

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