Recombinant Human Neuromodulin (GAP43)

Code CSB-YP009231HU
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Source Yeast
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Code CSB-EP009231HU
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Source E.coli
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Code CSB-EP009231HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009231HU
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Source Baculovirus
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Code CSB-MP009231HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GAP43
Uniprot No.
Alternative Names
Axonal membrane protein GAP 43; Axonal membrane protein GAP-43; B 50; Calmodulin binding protein P 57; F1; GAP 43; GAP43; Growth Associated Protein 43; Growth-associated protein 43; Nerve Growth Related Peptide; Nerve growth related peptide GAP43; NEUM_HUMAN; Neural phosphoprotein B 50; Neural phosphoprotein B-50; Neuromodulin; Neuron growth associated protein 43; PP46; Protein F1; QtrA-11580; QtrA-13071
Species
Homo sapiens (Human)
Expression Region
1-238
Target Protein Sequence
MLCCMRRTKQ VEKNDDDQKI EQDGIKPEDK AHKAATKIQA SFRGHITRKK LKGEKKDDVQ AAEAEANKKD EAPVADGVEK KGEGTTTAEA APATGSKPDE PGKAGETPSE EKKGEGDAAT EQAAPQAPAS SEEKAGSAET ESATKASTDN SPSSKAEDAP AKEEPKQADV PAAVTAAAAT TPAAEDAAAK ATAQPPTETG ESSQAEENIE AVDETKPKES ARQDEGKEEE PEADQEHA
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Gene References into Functions
  1. We found an increased expression of Nestin and GAP43 (growth associated protein 43) in treated cells. In conclusion, Periodontal ligament mesenchymal stem cells (hPDLSCs) treated with Moringin and Cannabidiol showed an improved survival capacity and neuronal differentiation potential PMID: 30096889
  2. The expression pattern of the regeneration-associated protein GAP-43 suggested a lower regenerative capacity in nigral dopaminergic neurons of Parkinson disease patients. PMID: 26748453
  3. Findings show high level of both YKL-40 and GAP-43 in CSF of older women with suicidal ideation which suggest that a disrupted synaptic glial functioning and inflammation may be related to the aetiology of suicidal ideation in older adults. PMID: 28211584
  4. associations of neuromodulin and neurogranin to Alzheimer's disease PMID: 27604409
  5. Copy-number variations are enriched for GAP43 and other neurodevelopmental genes in children with developmental coordination disorder. PMID: 27489308
  6. Downregulation of GAP43 promotes gliomas. PMID: 27495233
  7. The peripheral neuropathies lead to an initial increase in GAP-43 gene expression as a potential mechanism of regeneration, which is not sustained in neuropathies of long duration. PMID: 26071889
  8. Results show that PKC-dependent phosphorylation of GAP43 plays a critical role in regulating postsynaptic gephyrin aggregation in developing GABAergic synapses. PMID: 25755278
  9. increased expression of TH and GAP43 might be a molecular mechanism for left atrial myoelectricity remodeling of aging atrial fibrillation patients, which might be potential therapeutic targets of atrial fibrillation. PMID: 24301786
  10. GAP43 is seemingly a highly sensitive marker for peripheral nerve sheath tumors PMID: 23887302
  11. The results showed that the decreased GAP-43 levels induced by glutamate could be partially reversed by the presence of NRG-1beta PMID: 23524246
  12. Dynamic palmitoylation links cytosol-membrane shuttling of acyl-protein thioesterase-1 and acyl-protein thioesterase-2 with that of proto-oncogene H-ras product and growth-associated protein-43 PMID: 23396970
  13. immunostaning for GAP-43 was relatively similar in ganglionic versus aganglionic colon. PMID: 23153097
  14. Impaired regeneration of intra-epidermal C fibers in the early stages of type 2 diabetes mellitus, as indicated by GAP-43 expression, might be a marker of incipient diabetic neuropathy. PMID: 22209024
  15. The results of this study supported to the hypothesis of multiple rare mutations in schizophrenia, and it provides genetic clues that indicate the involvement of GAP-43 in this disorder. PMID: 22138049
  16. Through MEK/ERK pathway, S1P stimulates GAP43 transcription with increased binding of C/EBPbeta to the 5'-promoter PMID: 21769916
  17. Results demonstrate that APT-2 is the protein thioesterase involved in the acylation/deacylation cycle operating in GAP-43 subcellular distribution. PMID: 21152083
  18. Results indicate that there is no strong and direct interaction between POP and GAP43 at physiological conditions. PMID: 20869470
  19. Around 1 month post lesion, degeneration at the cochlear nuclei progressively disappeared and a relevant GAP-43 expression was found. PMID: 19593683
  20. chemical analysis of fatty acylated species in GAP43 PMID: 12105219
  21. In the dermis, there were fewer GAP-43 nerve fibers than PGP 9.5 fibers, whereas in the epidermis the numbers were equal. Only some Merkel cells and Meissner corpuscles were GAP-43-immunoreactive. PMID: 12704705
  22. Data show that GAP43 acts as an osmosensory protein that augments internal calcium in response to hypotonicity. PMID: 12805215
  23. Schizophrenia had significant decreases in GAP-43 immunoreactivity in the hilus (p<0.05, paired t-test) and inner molecular layer (p<0.05, paired t-test) but not in the outer molecular layer. In the same tissues. PMID: 15694236
  24. Growth-associated protein 43, a marker of neural outgrowth and regeneration, is expressed in endometriosis-associated nerve fibers but not in existing peritoneal nerves. PMID: 17412328
  25. HuD plays a role in the post-transcriptional control of GAP-43 mRNA PMID: 17577668
  26. Data show that functional cooperation between TrkA and p75(NTR) accelerates neuronal differentiation by increased transcription of GAP-43 and p21(CIP/WAF) genes via ERK1/2 and AP-1 activities. PMID: 17619016

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Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cytoplasm.
Protein Families
Neuromodulin family
Database Links

HGNC: 4140

OMIM: 162060

KEGG: hsa:2596

STRING: 9606.ENSP00000377372

UniGene: Hs.134974

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