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GAP43 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA269477A0HU
Size US$210
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Image
  • Immunofluorescence staining of Hela cell with CSB-RA269477A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA269477A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P17677
Target Names
GAP43
Alternative Names
Axonal membrane protein GAP 43 antibody; Axonal membrane protein GAP-43 antibody; B 50 antibody; Calmodulin binding protein P 57 antibody; F1 antibody; GAP 43 antibody; GAP43 antibody; Growth Associated Protein 43 antibody; Growth-associated protein 43 antibody; Nerve Growth Related Peptide antibody; Nerve growth related peptide GAP43 antibody; NEUM_HUMAN antibody; Neural phosphoprotein B 50 antibody; Neural phosphoprotein B-50 antibody; Neuromodulin antibody; Neuron growth associated protein 43 antibody; PP46 antibody; Protein F1 antibody; QtrA-11580 antibody; QtrA-13071 antibody
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human GAP43
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
2D8
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, IF, FC
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Growth-associated protein 43 (GAP43) serves as a critical marker of neuronal development and plasticity, playing essential roles in axonal growth, regeneration, and synaptic remodeling. This phosphoprotein, also known as neuromodulin or B-50, is highly expressed in neuronal growth cones and has become indispensable for researchers investigating neurodevelopmental processes, nerve injury repair, and neurodegenerative conditions.

This recombinant monoclonal antibody, clone 2D8, offers the reproducibility and consistency that demanding neuroscience research requires. Developed using recombinant technology with a defined sequence, this rabbit IgG antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The antibody was raised against a synthetic peptide derived from human GAP43, ensuring precise epitope targeting.

Validation studies demonstrate reliable performance across multiple detection platforms. Immunofluorescence analysis of HeLa cells reveals clear cytoplasmic staining patterns at dilutions ranging from 1:50 to 1:200, providing flexibility to optimize signal intensity for your specific imaging setup. Flow cytometry validation using the neuroblastoma cell line SH-SY5Y, a well-established neuronal model, shows distinct positive population shifts compared to isotype controls at 1:100 dilution, confirming the antibody's utility for quantitative single-cell analysis of GAP43 expression.

The antibody is supplied in a stabilized liquid format containing glycerol for convenient long-term storage at -20°C or -80°C without repeated freeze-thaw cycles. Affinity purification ensures high specificity while minimizing background interference.

Whether you are characterizing neural differentiation protocols, investigating axonal regeneration following injury, or studying synaptic plasticity mechanisms, this GAP43 antibody provides the technical reliability needed to advance your neuroscience research with confidence.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
Gene References into Functions
  1. We found an increased expression of Nestin and GAP43 (growth associated protein 43) in treated cells. In conclusion, Periodontal ligament mesenchymal stem cells (hPDLSCs) treated with Moringin and Cannabidiol showed an improved survival capacity and neuronal differentiation potential PMID: 30096889
  2. The expression pattern of the regeneration-associated protein GAP-43 suggested a lower regenerative capacity in nigral dopaminergic neurons of Parkinson disease patients. PMID: 26748453
  3. Findings show high level of both YKL-40 and GAP-43 in CSF of older women with suicidal ideation which suggest that a disrupted synaptic glial functioning and inflammation may be related to the aetiology of suicidal ideation in older adults. PMID: 28211584
  4. associations of neuromodulin and neurogranin to Alzheimer's disease PMID: 27604409
  5. Copy-number variations are enriched for GAP43 and other neurodevelopmental genes in children with developmental coordination disorder. PMID: 27489308
  6. Downregulation of GAP43 promotes gliomas. PMID: 27495233
  7. The peripheral neuropathies lead to an initial increase in GAP-43 gene expression as a potential mechanism of regeneration, which is not sustained in neuropathies of long duration. PMID: 26071889
  8. Results show that PKC-dependent phosphorylation of GAP43 plays a critical role in regulating postsynaptic gephyrin aggregation in developing GABAergic synapses. PMID: 25755278
  9. increased expression of TH and GAP43 might be a molecular mechanism for left atrial myoelectricity remodeling of aging atrial fibrillation patients, which might be potential therapeutic targets of atrial fibrillation. PMID: 24301786
  10. GAP43 is seemingly a highly sensitive marker for peripheral nerve sheath tumors PMID: 23887302
  11. The results showed that the decreased GAP-43 levels induced by glutamate could be partially reversed by the presence of NRG-1beta PMID: 23524246
  12. Dynamic palmitoylation links cytosol-membrane shuttling of acyl-protein thioesterase-1 and acyl-protein thioesterase-2 with that of proto-oncogene H-ras product and growth-associated protein-43 PMID: 23396970
  13. immunostaning for GAP-43 was relatively similar in ganglionic versus aganglionic colon. PMID: 23153097
  14. Impaired regeneration of intra-epidermal C fibers in the early stages of type 2 diabetes mellitus, as indicated by GAP-43 expression, might be a marker of incipient diabetic neuropathy. PMID: 22209024
  15. The results of this study supported to the hypothesis of multiple rare mutations in schizophrenia, and it provides genetic clues that indicate the involvement of GAP-43 in this disorder. PMID: 22138049
  16. Through MEK/ERK pathway, S1P stimulates GAP43 transcription with increased binding of C/EBPbeta to the 5'-promoter PMID: 21769916
  17. Results demonstrate that APT-2 is the protein thioesterase involved in the acylation/deacylation cycle operating in GAP-43 subcellular distribution. PMID: 21152083
  18. Results indicate that there is no strong and direct interaction between POP and GAP43 at physiological conditions. PMID: 20869470
  19. Around 1 month post lesion, degeneration at the cochlear nuclei progressively disappeared and a relevant GAP-43 expression was found. PMID: 19593683
  20. chemical analysis of fatty acylated species in GAP43 PMID: 12105219
  21. In the dermis, there were fewer GAP-43 nerve fibers than PGP 9.5 fibers, whereas in the epidermis the numbers were equal. Only some Merkel cells and Meissner corpuscles were GAP-43-immunoreactive. PMID: 12704705
  22. Data show that GAP43 acts as an osmosensory protein that augments internal calcium in response to hypotonicity. PMID: 12805215
  23. Schizophrenia had significant decreases in GAP-43 immunoreactivity in the hilus (p<0.05, paired t-test) and inner molecular layer (p<0.05, paired t-test) but not in the outer molecular layer. In the same tissues. PMID: 15694236
  24. Growth-associated protein 43, a marker of neural outgrowth and regeneration, is expressed in endometriosis-associated nerve fibers but not in existing peritoneal nerves. PMID: 17412328
  25. HuD plays a role in the post-transcriptional control of GAP-43 mRNA PMID: 17577668
  26. Data show that functional cooperation between TrkA and p75(NTR) accelerates neuronal differentiation by increased transcription of GAP-43 and p21(CIP/WAF) genes via ERK1/2 and AP-1 activities. PMID: 17619016

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Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane; Peripheral membrane protein. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cytoplasm.
Protein Families
Neuromodulin family
Database Links

HGNC: 4140

OMIM: 162060

KEGG: hsa:2596

STRING: 9606.ENSP00000377372

UniGene: Hs.134974

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