Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

VGF

Uniprot No.

O15240

Research Area

Neuroscience

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

23-206aa

Target Protein Sequence

APPGRPEAQPPPLSSEHKEPVAGDAVPGPKDGSAPEVRGARNSEPQDEGELFQGVDPRALAAVLLQALDRPASPPAPSGSQQGPEEEAAEALLTETVRSQTHSLPAPESPEPAAPPRPQTPENGPEASDPSEELEALASLLQELRDFSPSSAKRQQETAAAETETRTHTLTRVNLESPGPERVW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

54.6 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

In expressing the recombinant Human VGF protein, e.coli cells are transfected with a DNA expression vector that harbors the gene for the desired protein (23-206aa). The cultured cells then express the intended protein. The protein is equipped with a N-terminal 10xHis-GST tag and C-terminal Myc tag. The recombinant Human VGF protein is collected and purified from the cell lysate through affinity purification, achieving a purity surpassing 85%, as confirmed by SDS-PAGE.

Target Background

Function

Secreted polyprotein that is packaged and proteolytically processed by prohormone convertases PCSK1 and PCSK2 in a cell-type-specific manner. VGF and peptides derived from its processing play many roles in neurogenesis and neuroplasticity associated with learning, memory, depression and chronic pain.; Plays a role in the control of body fluid homeostasis by regulating vasopressin release. Suppresses presynaptic glutamatergic neurons connected to vasopressin neurons.; Plays a role in the control of body fluid homeostasis by regulating vasopressin release. Activates GABAergic interneurons which are inhibitory neurons of the nervous system and thereby suppresses presynaptic glutamatergic neurons. Stimulates also feeding behavior in an orexin-dependent manner in the hypothalamus. Functions as a positive regulator for the activation of orexin neurons resulting in elevated gastric acid secretion and gastric emptying.; Secreted multifunctional neuropeptide that binds to different cell receptors and thereby plays multiple physiological roles including modulation of energy expenditure, pain, response to stress, gastric regulation, glucose homeostasis as well as lipolysis. Activates the G-protein-coupled receptor C3AR1 via a folding-upon-binding mechanism leading to enhanced lipolysis in adipocytes. Interacts with C1QBP receptor in macrophages and microglia causing increased levels of intracellular calcium and hypersensitivity.; Plays a role in the regulation of memory formation and depression-related behaviors potentially by influencing synaptic plasticity and neurogenesis. Induces acute and transient activation of the NTRK2/TRKB receptor and subsequent CREB phosphorylation. Induces also insulin secretion in insulinoma cells by increasing intracellular calcium mobilization.; Has bactericidal activity against M. luteus, and antifungal activity against P. Pastoris.

Gene References into Functions

Results show that VGF is epigenetically modified in human non-small-cell lung cancer (NSCLC) tissues if compared to tumor-free lung tissues, which results in an increased transcription and protein expression. PMID: 29209432
results suggest VGF enhances dendritic maturation and that these effects can be altered by common SNPs in the VGF gene PMID: 28287464
We conclude that some of the already identified variants in VGF from human polymorphism studies may contribute to eating disorders and obesity. PMID: 27088090
Data show although no any significant differences between patient groups and lean subjects of proteins SYT4, BAG3, APOA1, and VAV3, except for VGF protein, there was a trend between the expression of these four genes and their protein levels. PMID: 26337083
Data show that two VGF peptides (NAPP-19 and QQET-30) were identified in plasma. PMID: 26562304
Data indicate an increased number of neurosecretory protein VGF-expressing T cells in patients with Alzheimer's disease (AD) compared to aged healthy controls. PMID: 26142708
Results indicate that neuron-restrictive silencer factor plays an important role as a repressor of VGF gene regulation in neuroblastoma cells through a mechanism that is dependent on VGF-neuron-restrictive silencer element PMID: 25569790
NERPs may be potent endogenous suppressors of glucose-dependent insulin secretion. PMID: 25529453
DISC1 knockdown leads to a reduction of VGF in neurons. PMID: 24934694
Knock-in mice expressing human VGF were fertile, had increased body weight, whereas those with c-terminal region deletion had reduced adiposity, increased energy expenditure, and improved glucose tolerance. PMID: 25675362
We conclude that VGF contributes to survival and function of peripheral T cells PMID: 25013207
Among the 19 genes tested, VGF was found to be completely methylated in several Urothelial Cell Carcinoma cell lines PMID: 24830820
The expression of NPY and VGF was increased in the arcuate nucleus, but decreased in the nucleus of the Tractus Solitarius in the brains of type-II diabetic patients. PMID: 22808091
[review] The vgf gene is induced in vivo by neurotrophins including nerve growth factor (NGF), brain derived growth factor (BDNF) and glial derived growth actor (GDNF), by synaptic activity and by homeostatic and other stimuli. PMID: 21621608
VGF is regulated by SOD1 and plays a critical role in motor neuron survival PMID: 21151573
localization of neuroendocrine regulatory peptide-1 and-2 (NERP-1 and NERP-2); results suggest that neuroendocrine NERP-1 and NERP-2 might function as local modulatorsin the neuroendocrine system PMID: 20471433
VGF is downregulated in bipolar disorder in the CA region of the hippocampus and Brodmann's area 9 of the prefrontal cortex. PMID: 20631166
VGF mRNA levels were significantly reduced in drug-free depressed patients, as compared with controls, and were modulated in response to effective antidepressant treatment. PMID: 20164831
Application of neurosecretory protein VGF biomarker model to current diagnostic criteria provides an objective biomarker pattern that identifies patients with amyotrophic lateral sclerosis. PMID: 16481598
proVGF-related peptides are present in endocrine cells early during development and adulthood and increase in hyperplasia and tumors PMID: 17440014
while Vgf may be a reliable biomarker of progression of muscle weakness in patients with ALS, restoration of Vgf expression in spinal cord motor neurons may therapeutically rescue spinal cord motorneurons against excitotoxic injury PMID: 18432310

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Subcellular Location

[Neurosecretory protein VGF]: Secreted. Cytoplasmic vesicle, secretory vesicle. Note=Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.

Tissue Specificity

Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.

Database Links

HGNC: 12684

OMIM: 602186

KEGG: hsa:7425

STRING: 9606.ENSP00000249330

UniGene: Hs.587325

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Code	CSB-EP025847HU1
Abbreviation	Recombinant Human VGF protein, partial
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Neurosecretory protein VGF (VGF), partial

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