Recombinant Human Nuclear transcription factor Y subunit beta(NFYB)

Code CSB-EP015774HU
Size US$2466
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names NFYB
Uniprot No. P25208
Research Area Signal Transduction
Alternative Names CAAT box DNA binding protein subunit B; CAAT box DNA-binding protein subunit B; CBF A; CBF B; CCAAT binding transcription factor subunit A; HAP 3; HAP3; NF Y protein chain B; NF YB; NF-YB; NFYB; NFYB_HUMAN; Nuclear transcription factor Y beta; Nuclear transcription factor Y subunit B; Nuclear transcription factor Y subunit beta; Transcription factor NF Y B subunit
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-207aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 49.8kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Gene References into Functions
  1. The nuclear transcription factor Y subunit beta (NFYB)-E2F transcription factor 1 (E2F1) pathway displays a crucial role in the chemoresistance ofoxaliplatin-resistant colorectal cancer (OR-CRC) by inducing the expression and activation of checkpoint kinase 1 (CHK1), suggesting a possible therapeutic target for oxaliplatin resistance in CRC. PMID: 29203250
  2. NF-YB transcription factor was identified as a novel direct E2F1 target. PMID: 26039627
  3. The NF-Y complex is asymmetric, with NF-YB binding approximately 15 bp downstream from the CCAAT motif. PMID: 23595228
  4. Transcriptional repression mediated by IER5 regulates Cdc25B expression levels via the release of NF-YB and p300 in acute myeloid leukemia. PMID: 22132193
  5. NF-Y acts upstream of H3K4me3 deposition by recruiting Ash2L PMID: 21445285
  6. MicroRNA-485-3p regulates drug responsiveness by decreasing nuclear factor-YB expression, which in turn negatively regulates DNA topoisomerase IIalpha. PMID: 21252292
  7. The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y. PMID: 12401788
  8. CBF/NF-Y proteins regulate the transcription of COL11A1 by directly binding to the ATTGG sequence in the proximal promoter region PMID: 12805369
  9. Co-operative functional interactions between Sp1 & NF-Y are required to direct the activity of the ATP1A3 promoter predominantly in neuronal cells. PMID: 15462673
  10. trichostatin A activates the transcription of TSP1 gene through the binding of transcription factor CBF to CCAAT box and the enhanced histone acetylation. PMID: 18275041
  11. CBF-A is a novel transacting factor required for cytoplasmic mRNA transport and localization PMID: 18480411

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Subcellular Location Nucleus
Protein Families NFYB/HAP3 subunit family
Database Links

HGNC: 7805

OMIM: 189904

KEGG: hsa:4801

STRING: 9606.ENSP00000240055

UniGene: Hs.84928


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