Recombinant Human Palmitoyl-protein thioesterase 1 (PPT1)

Code CSB-YP018587HU
MSDS
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Source Yeast
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Code CSB-EP018587HU
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Source E.coli
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Code CSB-EP018587HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018587HU
MSDS
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Source Baculovirus
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Code CSB-MP018587HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PPT1
Uniprot No.
Alternative Names
Ceroid palmitoyl palmitoyl protein thioesterase 1; CLN1; EC 3.1.2.22; INCL; Palmitoyl protein hydrolase 1 ; Palmitoyl protein thioesterase 1; Palmitoyl-protein hydrolase 1; Palmitoyl-protein thioesterase 1; PPT; PPT-1; PPT1; PPT1_HUMAN
Species
Homo sapiens (Human)
Expression Region
28-306
Target Protein Sequence
DPP APLPLVIWHG MGDSCCNPLS MGAIKKMVEK KIPGIYVLSL EIGKTLMEDV ENSFFLNVNS QVTTVCQALA KDPKLQQGYN AMGFSQGGQF LRAVAQRCPS PPMINLISVG GQHQGVFGLP RCPGESSHIC DFIRKTLNAG AYSKVVQERL VQAEYWHDPI KEDVYRNHSI FLADINQERG INESYKKNLM ALKKFVMVKF LNDSIVDPVD SEWFGFYRSG QAKETIPLQE TSLYTQDRLG LKEMDNAGQL VFLATEGDHL QLSEEWFYAH IIPFLG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
Gene References into Functions
  1. Targeting PPT1 blocks mTOR signaling in a manner distinct from catalytic inhibitors, while concurrently inhibiting autophagy, thereby providing a new strategy for cancer therapy. PMID: 28899863
  2. the combination of elevated glycolysis and deficient MRPL13 activity was closely linked to CLN1-mediated tumor activity in human hepatoma cells PMID: 28978646
  3. Proteomics analysis on isolated cilia revealed 660 proteins, which differed in their abundance levels between wild type and Ppt1 knock out. PMID: 28334871
  4. we reveal the existence of a positive feedback loop, where palmitoylation of PPT1 results in decreased activity and subsequent elevation in the amount of palmitoylated proteins. PMID: 26731412
  5. analysis of the palmitoyl protein thioesterase 1 interactome in SH-SY5Y human neuroblastoma cells PMID: 25865307
  6. Data (including data from knockout mice) suggest that deficiency of PPT1 leads to accumulation of granular osmiophilic deposits in many cell types, especially in astrocytes. [review-like article] PMID: 25233404
  7. Data suggest that human monocytes and macrophages express PPT1; PPT1 appears to contribute 32-40% of 2-arachidonylglycerol hydrolysis activity in THP1 monocyte cell line. PMID: 24083319
  8. This neuroimaging finding in PPT1-related neuronal ceroid lipofuscinosis was not previously reported. PMID: 22520356
  9. Stop codon read-through with PTC124 induces palmitoyl-protein thioesterase-1 activity, reduces thioester load and suppresses apoptosis in cultured cells from Infantile neuronal ceroid lipofuscinosis patients. PMID: 21704547
  10. Results describe the correlation between the three-dimensional structural changes in mutant palmitoyl protein thioesterase 1 and biochemical phenotypes. PMID: 19793631
  11. mutated in neuronal ceroid lipofuscinosis PMID: 12025857
  12. The clinical, biochemical, and molecular genetic aspects of lysosomal storage disorders are discussed in this review PMID: 12125808
  13. The crystal structure of palmitoyl protein thioesterase-2 (PPT2) reveals the basis for divergent substrate specificities of the two lysosomal thioesterases, PPT1 and PPT2. PMID: 12855696
  14. there is a close correlation between CLN2 and CLN1 expression and colorectal carcinoma progression and metastasis and suggest that they may be potential molecular targets PMID: 16518810
  15. Results show that PPT1-deficiency causes a defect in fluid-phase and receptor-mediated endocytosis. PMID: 16542649
  16. ER stress due to PPT1-deficiency increases ROS and disrupts calcium homeostasis activating caspase-9 and caspase-9 activation mediates caspase-3 activation and apoptosis contributing to rapid neurodegeneration in INCL. PMID: 16571600
  17. Adult neuronal ceroid lipofuscinosis caused by deficiency in palmitoyl protein thioesterase 1. PMID: 17261688
  18. Palmitoyl protein thioesterase-1 deficiency impairs synaptic vesicle recycling at nerve terminals in humans and mice PMID: 18704195
  19. study presents clinical and diagnostic investigations in six children with variant late infantile neuronal ceroid lipofuscinosis and mutations in CLN1 including a new large-scale deletion on one allele PMID: 19302939
  20. Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527
  21. The function of PPT1 is to hydrolyze long-chain fatty acids from cysteine residues of fatty acylated proteins in the lysosome. PMID: 8816748

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Involvement in disease
Ceroid lipofuscinosis, neuronal, 1 (CLN1)
Subcellular Location
Lysosome. Secreted.
Protein Families
Palmitoyl-protein thioesterase family
Database Links

HGNC: 9325

OMIM: 256730

KEGG: hsa:5538

STRING: 9606.ENSP00000394863

UniGene: Hs.3873

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