Recombinant Human Peroxisome prolifeRator-activated receptor gamma(PPARG)

Code CSB-YP018424HU
Size US$1916
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names PPARG
Uniprot No. P37231
Research Area Transcription
Alternative Names CIMT1; GLM1; NR1C3; Nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; Peroxisome proliferator activated nuclear receptor gamma variant 1; Peroxisome proliferator activated receptor gamma 1; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARG; PPARG_HUMAN; PPARG1; PPARG2; PPARgamma
Species Homo sapiens (Human)
Source Yeast
Expression Region 1-477aa
Target Protein Sequence MTMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 56.3kDa
Protein Length Full Length of Isoform 1
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).
Gene References into Functions
  1. LEPR rs1137101 and PPARG-2 rs1801282 had weak and medium negative effects on zBMI, respectively, and may slightly protect against childhood obesity. PMID: 29679223
  2. Data show the partial-agonist-bound PPARgamma is characterized by multiple thermodynamically accessible conformations. PMID: 29728618
  3. Cellular metabolism constrains innate immune responses in preterm infants due to perturbations in the expression of PPARgamma, MALT1, DDIT4, and most of the cytokines. PMID: 30446641
  4. PPARgamma knockdown resulted in increased levels of TOMM40, APOE and APOC1 -mRNAs, showing the strongest impact on APOE transcript levels. PMID: 28065845
  5. Studies indicate that peroxisome proliferator activated receptor gamma (PPARgamma) expression is found in different regions of the kidney and, upon activation, can redirect metabolism [Review]. PMID: 30012954
  6. These results demonstrate that betaine acts through ERK1/2-PPARgamma signalling pathway to regulate lipid metabolism in adipogenic-differentiated skeletal muscle cells, which could provide some useful information for controlling muscle lipid accumulation by manipulating ERK1/2 and PPARgamma signalling pathway. PMID: 29383561
  7. novel soluble form present in maternal plasma, umbilical plasma, and amniotic fluid, elevated in gestational diabetes mellitus maternal individuals, and correlated with the lipid uptake of trophoblast cells PMID: 28720051
  8. ALDH1A3 suppression could be one of PPARG tumor suppressive function. This study provides a better understanding of the role of PPARG in lung cancer. PMID: 29873276
  9. Regulation of transmembrane-4-L-six-family-1 (TM4SF1) on bladder cancer cell could be induced by peroxisome proliferator-activated receptor gamma (PPARgamma)-sirtuin 1 (SIRT1) feedback loop. PMID: 29175458
  10. Peroxisome proliferators-activated receptor gamma polymorphism is associated with the risk of colorectal cancer. PMID: 29970681
  11. The lack of PPARγ in recurrent miscarriage decidual macrophages seems to be associated with a specific inflammatory response against the fetus. PMID: 29949879
  12. EDF1 is required for VEGF-induced activation of the transcriptional activity of PPARgamma in HUVEC cells. PMID: 29933613
  13. Studied peroxisome proliferator-activated receptor-gamma gene (PPARgamma) single nucleotide polymorphisms (SNPs); found the SNPs to be associated with the occurrence or progression of sepsis. PMID: 29055064
  14. This study suggests that T2D patients with different genotypes at CD36, NOS3 and PPARG respond differentially to intervention of omega-3 supplements in blood lipid profiles. PMID: 29703528
  15. ITLN1, PPARg AND TNFa GENE EXPRESSION IN VISCERAL ADIPOSE TISSUE. PMID: 30188100
  16. Gene expressions of YKL-40 and CD36 were significantly higher in patients with T2DM (>5 yr) with hypertension compared to healthy controls (P=0.006). In addition, a significant increase in serum levels of sCD36, PPAR-gamma and YKL-40 was observed in patients with T2DM (>5 yr) with hypertension compared to healthy controls PMID: 29806605
  17. Mechanistic investigation revealed a miR-301b~miR-130b-PPARgamma axis, whose expression pattern in umbilical cord (UC), adipose tissue (Ad) and bone marrow mesenchymal stem cells significantly correlates with their adipogenic capacity. PMID: 28442776
  18. The negative responders for aerobic training are subjects with the PPARG rs1801282 Pro/Pro genotype. The best responders to aerobic training are PPARG rs1801282 Ala carriers. PMID: 29762540
  19. These results demonstrated the association of PPARgamma Pro allele with susceptibility to acne vulgaris. PMID: 29120856
  20. participation rates in the genotype-based recall study were 31%, 44%, and 40%, and accordingly we included 12, 15, and 13 individuals with Pro12Pro, Pro12Ala, and Ala12Ala variants of PPARG, respectively PMID: 29303622
  21. Here we have shown for the first time, that ligand- or insulin-mediated activation of PPARgamma in human hepatoma cell line HepG2 causes the downregulation of C3 gene expression and protein secretion PMID: 29550264
  22. Interactions between SOD2 and PPAR-gamma SNPs regarding mortality influence were detected in diabetic ESRD patients. PMID: 27925431
  23. Data suggest that up-regulation of NPY inhibits proliferation of adipose-derived stem cells while promoting adipogenesis and up-regulating expression of white adipocyte biomarkers PPARG, CEBPA, CIDEC, and RIP140. (NPY = neuropeptide Y; CEBPA = CCAAT/enhancer-binding protein alpha; CIDEC = cell death-inducing DFFA-like effector C; RIP140 = nuclear receptor interacting protein 1) PMID: 28954935
  24. The presence of Pro12Pro and C14131C genotypes is related to higher TAG level in CTD. PMID: 29606859
  25. Low PPARG expression is associated with childhood obesity. PMID: 28733963
  26. The results propose that PPARgamma may act as a master regulator of the transcription of several genes involved in late-onset Alzheimer's disease pathogenesis. PMID: 29723294
  27. Polymorphism of PPARG is associated with late onset of type 2 diabetes mellitus. PMID: 28292576
  28. PPARgamma gene rs2921190 polymorphism was correlated with the susceptibility to northwest dryness syndrome. PMID: 29960292
  29. Phosphorylation of S273 of PPARgamma occurs in cancer cells on exposure to DNA damaging agents. PMID: 29295932
  30. Chinese Han persons with C allele of rs3856806 in PPARG gene were significantly associated with the decreased risk of hypertension. PMID: 29266977
  31. We further identified this underlying mechanism also involved a PPARgamma-induced ANXA1-dependent autoubiquitination of cIAP1, the direct E3 ligase of RIP1, shifting cIAP1 toward proteosomal degradation..our study provides first insight for the suitability of using drug-induced expression of ANXA1 as a new player in RIP1-induced death machinery in triple-negative breast cancer PMID: 29021293
  32. PAX8-PPARG fusions may not play major roles in the tumorigenesis of paediatric follicular thyroid carcinoma PMID: 28621837
  33. Quantitative polymerase chain reaction, immunohistochemistry, Western blotting, and microarray analysis showed enrichment of adipogenic mediators (C/EBP family P = .027; KLF5 P < .000; and peroxisome proliferator activated receptor-gamma, P = .032) in AAA tissue. PMID: 28912007
  34. the mRNA expression levels of PPARG were not significantly different between the tumor tissues and tissue margins PMID: 28504924
  35. These results suggest that the inhibited brain development seen in humans exposed to the stress of a premature extrauterine environment is modulated by genetic factors, and that PPARG signaling has a previously unrecognized role in cerebral development. PMID: 29229843
  36. PPAR-gamma and Akt regulate GLUT1 and GLUT3 surface localization during Mycobacterium tuberculosis infection. PMID: 28852964
  37. Here, the authors characterize mutant RXRA, demonstrating it induces enhancer/promoter activity in the context of RXRA/PPAR heterodimers in human bladder cancer cells. PMID: 29143738
  38. Low PPARG expression is associated with Gemcitabine Resistance in Cholangiocarcinoma. PMID: 28560603
  39. PPARG gene polymorphism causes the development of oxidative stress in patients with type 2 diabetes with 5-10 years durations. PMID: 29762970
  40. co-expression of PPARgamma and TRAP220 represents a biomarker for good prognosis in colorectal cancer patients PMID: 26130665
  41. Carriers of the PPAR-gamma Ala12 allele showed greater cognitive decline compared to non-carriers but the risk varied across sex and ethnic groups. Male Ala12 carriers of Hispanic origin may be a high-risk group for cognitive decline. PMID: 28181642
  42. overexpression of GATA3 and FOXA1 cooperate with PPAR activation to drive transdifferentiation of a basal bladder cancer cells to a luminial phenotype. PMID: 27924948
  43. Results show that the nuclear distribution of PPARgamma in gastric cancer (GC) tissues and cell lines was markedly decreased. Its expression is regulated by miR-133b in gastric neoplasm tissue and cell lines. PMID: 28901466
  44. study implies that DJ-1 may protect endothelial progenitor cells against Ang II-induced dysfunction by activating the PPARgamma/HO-1. PMID: 28600848
  45. There were no differences between the distribution of genotypes and the allele frequencies of the PPARG Pro12Ala, and C1431T polymorphisms and the ADRB3 Trp64Arg polymorphism in normo- and hyperglycaemic women. PMID: 29464546
  46. We conclude that rs1784933 and rs1805192 minor alleles, gene- gene interaction between rs1784933 and rs1805192, gene- environment interaction between rs1784933 and alcohol drinking, and haplotype containing the rs1784933- A and rs689021- C alleles are all associated with increased LOAD risk. PMID: 28427149
  47. Most of the tested flavonoids, but not the antioxidant vitamins, stimulated Nrf2-, FoxO-, and PPARgamma-dependent promoter activities. PMID: 28761622
  48. Findings suggest that despite their proposed involvement in the regulation of inflammatory processes in multiple sclerosis (MS), PPARalpha, PPARbeta/delta, and PGC-1alpha proteins are not potential biomarkers of neuroinflammation in MS, and indicate a preferential role of PPARgamma in the endogenous regulation of autoimmune response in the human central nervous system within its receptor family. PMID: 28483651
  49. combined administration of small-interfering RNA (siRNA) transfection with PPARgamma ligands induced downregulation of SOX2 and MMP2 activity together with inhibition of sphere-forming activity regardless of poly(ADP-ribose) polymerase (PARP) cleavage. Taken together, our findings suggest that a combination therapy using PPARgamma ligands and its inhibitor could be a potential therapeutic strategy targeting glioblastom... PMID: 28642874
  50. Results showed that polymorphism in PPARGrs10865710, PPARGrs1805192 and CYP1A1rs4646903 and interaction between PPARGrs1805192 and CYP1A1rs4646903 were related with increased coronary artery disease susceptibility. PMID: 28415751
  51. High concentrations of DINCH urinary metabolites activate human PPAR-gamma. PMID: 29421333
  52. UCP2 is a key mediator of hypoxia-triggered chemoresistance in non-small cell lung cancer cells via repression of peroxisome proliferator-activated receptor gamma. PMID: 28042952
  53. MERS-CoV S protein binds to DPP4 to suppress macrophage activation via induction of IRAK-M, PPARgamma and the immunosuppressive cytokine IL-10. PMID: 28118607
  54. our findings provide novel insights into the role of PPARgamma in inhibiting breast cancer progression PMID: 27556298
  55. these findings identified an important role of renal tubular epithelium-targeted PPAR-gamma in maintaining the normal epithelial phenotype and opposing fibrogenesis, possibly via antagonizing oxidative stress PMID: 27602490
  56. Results demonstrate that phosphorylation of PPARgamma at Ser84 is up-regulated in hepatocellular carcinoma (HCC) tumors. The study provides the first evidence that the novel roles of PPARgamma are linked to glycolysis by PFKFB4 for the regulation of glycolysis and proliferation in HCC cells. PMID: 27769068
  57. These results indicated that AKT-PPARgamma signaling pathway mediated HG-induced lipid deposition by upregulating CD36 expression in HK-2 cells and that inhibition of AKT-PPARgamma signaling pathway had the potential beneficial effects of reducing lipid deposition in diabetic kidney. PMID: 28497039
  58. Consumption of a high-fat, high-calorie meal is associated with an increase in intracellular co-localization of PPARgamma mRNA and protein in monocytes. PMID: 27418556
  59. it was demonstrated that the risk of one of the diabetic complications, i.e., diabetic nephropathy, was associated with the variable rs1801282 locus of the PPARG2 gene. PMID: 29369552
  60. PPARgamma gene polymorphism was associated with serum hsCRP level and periodontitis as BMI increased in post-menopausal Japanese women. PMID: 24494816
  61. Treatment with peroxisome proliferator activated receptor gamma (PPARgamma) ligands significantly alters organic cation transporter-1 (OCT-1) activity in BCR-ABL1+ chronic myeloid leukemia (CML) cell lines. PMID: 28154092
  62. Data show that inhibition of MAPK pathway by peroxisome proliferator-activated receptor gemma (PPARg) agonist rosiglitazone (RGZ) requires Toll-like receptor 4 (TLR4). PMID: 27323819
  63. A preclinical proof of concept for PPARG as a candidate therapeutic target in bladder cancer. PMID: 28923856
  64. Taken together, PPAR gamma and FABP4 gene expression levels in PBMCs [peripheral blood mononuclear cells] may be indicators of metabolic factors and body composition components. PMID: 27563749
  65. Relatively modest fat loss in patients with PPARG mutations may render the recognition of the Familial partial lipodystrophy syndrome more difficult in routine clinical practice. The PPARG H449L mutation is associated with insulin resistance and metabolic complications, but their severity is variable among the affected subjects. PMID: 26756202
  66. These results suggest that PPARgamma can upregulate BCRP expression in the placenta, which may be important in understanding mechanisms that protect the fetus from xenobiotic exposure during development. PMID: 27879033
  67. Pharmacological experiments allowed us to demonstrate that these effects were driven by an Erk1/2-mediated activation of the antioxidant transcription factor PPARg. These results suggest that b3-AR protective effects in the myometrium could be due to its dual antioxidant properties. PMID: 28723418
  68. We show that elevated levels of PPARG strongly correlate with elevation of FASN in human prostate cancer (CaP) and that high levels of PPARG/FASN and PI3K/pAKT pathway activation confer a poor prognosis.These data suggest that CaP patients could be stratified in terms of PPARG/FASN and PTEN levels to identify patients with aggressive CaP who may respond favorably to PPARG/FASN inhibition. PMID: 27357679
  69. The PPARgamma-SETD8 axis constitutes an epigenetic, p53-independent checkpoint on p21-mediated cellular senescence. PMID: 28514051
  70. Study found a significant interaction between CYP2J2- rs890293 and PPAR -gamma- rs1805192 and participants with GT or TT of rs890293 and CG or GG of rs1805192 genotype have the highest late-onset Alzheimer's disease risk, compared to participants with GG of rs890293 and CC of rs1805192 genotype. PMID: 27396818
  71. Data suggest that MMP2 expression in lung neoplasms can be regulated by dietary factors; here, metabolites of quercetin (an antioxidant, anticarcinogenic dietary supplement) quercetin-3-glucuronide and quercetin 3'-sulfate down-regulates expression of MMP2 in A549 cells; the mechanism for this enzyme repression appears to involve up-regulation of PPARgamma. PMID: 27260467
  72. PPARgamma signaling in cystic fibrosis airway epithelial cells undergoing repair PMID: 27484450
  73. It is likely that PPARg2 gene polymorphism may be associated with residual C-peptide secretion and involve excessive BMI in type 1 diabetes. PMID: 26615044
  74. These findings suggest no significant association of p.Pro12Ala polymorphism with retinopathy in tested type 2 diabetic retinopathy patients as compared to type 2 diabetes mellitus individuals take as controls. PMID: 27427939
  75. Immune cell-infiltration is controlled by activated peroxisome proliferator activated receptor gamma (PPARgamma)/retinoid X receptor alpha (RXRalpha) that inhibits expression/secretion of inflammatory cytokines. PMID: 28740126
  76. Results show that resistin down-regulates the transcription of GLUT1 by suppressing the expression of PPARG, thus causing impaired glucose transportation in endothelial cell layers. PMID: 27353463
  77. these studies demonstrate that PPARgamma is an important modulator of MUC16 expression. PMID: 27292441
  78. a panel of proteins correlated with PPARgamma expression that could be associated with colorectal cancer, was identified. PMID: 27499265
  79. We propose a novel role of the miR-27a/PPARgamma/beta-catenin axis in fostering the progression toward more deteriorated podocyte injury in diabetic nephropathy (DN). Targeting miR-27a could be a potential therapeutic approach for DN. PMID: 28277542
  80. the presented cPCA mapping of the PPARg-ligand complexes may guide in silico drug discovery programs seeking to avoid stabilization of helix 12 in their development of partial- and non-agonistic PPARg ligands. PMID: 28543443
  81. The presence of the PPARgamma (Pro12Ala) SNP did not modify the association between inorganic arsenic methylation capacity and breast cancer. PMID: 27557380
  82. COX-2 is a potential marker for identifying high-risk sebaceous gland carcinoma (SGC) patients. Expression of PPAR-gamma in eyelid SGC cases reflects terminal sebaceous differentiation. PMID: 26791964
  83. PAK4 downregulation decreased PPARgamma-mediated Nox1 expression and suppressed EMT in IR-treated glioma cells. PMID: 28534509
  84. Aleglitazar protects cardiomyocytes against hyperglycaemia-induced apoptosis by combined activation of both peroxisome proliferator-activated receptor-alpha and peroxisome proliferator-activated receptor-gamma. PMID: 28111985
  85. a positive association of placental PPARgamma mRNA levels and placental DHA levels with baby weight PMID: 27578106
  86. we found that PPARdelta directly regulated neutral amino acid transporter SLC1-A5 (solute carrier family 1 member 5) and glucose transporter-1 (Glut1) gene transcription, leading to uptake of glucose and amino acid, activation of mTOR signaling, and tumor progression. In contrast, silence of PPARdelta or its antagonist inhibited this event. PMID: 28419191
  87. The Atgl is down-regulated by the basal transcription factor Sp1 in preadipocytes and that the magnitude of down-regulation depends on interactions between Sp1 and peroxisome proliferator-activated receptor gamma (PPARgamma). PMID: 28726642
  88. The studied polymorphisms were not associated with gestational diabetes in a Brazilian population. PMID: 28699988
  89. Altogether, the authors establish the first in vivo human angiomyolipoma model, which provides evidence that angiomyolipoma may originate in a PPARG-activated renal mesenchymal stem cell lineage that is skewed toward adipocytes and smooth muscle and away from osteoblasts, and uncover PPARG as a regulator of angiomyolipoma growth, which could serve as an attractive therapeutic target. PMID: 28275008
  90. Analysis of the PPARG gene in families with familial partial lipodystrophy PMID: 28641778
  91. These data demonstrate that complex of PPARgamma with GW1929 is a negative regulator involved in the control of ApoA-I expression and secretion in human hepatocyte- and enterocyte-like cells PMID: 26813964
  92. The Adiponectin increases AQP3 expression through PPARalpha-mediated signaling inhepatic stellate cells. PMID: 28595905
  93. studies in humans and mice reveal that Akt2 controls hepatic tumorigenesis through crosstalk between HNF1alpha and PPARgamma. PMID: 28394260
  94. In this study, we found that rs1805192 alleles and genotypes of the PPAR-gamma gene were associated with Systemic lupus erythematosus risk. PMID: 27324555
  95. we performed a candidate-gene association study in a young and sportive Italian population by testing the association of functional polymorphisms in ACE (rs4646994), FTO (rs9939609), MC4R (rs17782313) and PPARG (rs1801282) genes with body mass index (BMI) and waist-to-height ratio (WHtR). PMID: 28090739
  96. Missense variant in PPARG is associated with lipodystrophy and type 2 diabetes. PMID: 27749844
  97. High PPARgamma expression is associated with Acute Monocytic Leukemia. PMID: 28108519
  98. Data indicate that MMP1, MMP12 and PPARG and hsa-miR-31-5p, hsa-miR-224-5p, and hsa-miR-223-3p were able to distinguish tumors from NPT with high sensitivity and specificity. PMID: 28122331
  99. The immunoprecipitation results also showed that high AA concentrations significantly increased the interaction of mTOR and PPARg. In summary, PPARg plays an important role in the regulation of IGF-1 secretion and gene expression in response to dietary protein. PMID: 28257428
  100. PPARgamma2 C1431T polymorphism affects the antiobesogenic effects of kochujang, asSoybean-based red pepper paste, in overweight and obese groups. PMID: 28622115

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Involvement in disease Obesity (OBESITY); Lipodystrophy, familial partial, 3 (FPLD3); Glioma 1 (GLM1)
Subcellular Location Nucleus, Cytoplasm
Protein Families Nuclear hormone receptor family, NR1 subfamily
Tissue Specificity Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Database Links

HGNC: 9236

OMIM: 137800

KEGG: hsa:5468

STRING: 9606.ENSP00000287820

UniGene: Hs.162646

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