Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
PHOP1_HUMAN; Phosphatase orphan 1; Phospho 1 ; phospho1; Phosphoethanolamine/phosphocholine phosphatase
Species
Homo sapiens (Human)
Expression Region
1-267aa
Target Protein Sequence
MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDENSDDSIVRAAPGQRLPESLRATYREGFYNEYMQRVFKYLGEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEVILISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGLLALRPFHTHSCARCPANMCKHKVLSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEPSSFRASVVPWETAADVRLHLQQVLKSC
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 1-267 form the expressed segment for recombinant Human PHOSPHO1. The expected molecular weight for the PHOSPHO1 protein is calculated to be 33.7 kDa. Expression of this PHOSPHO1 protein is conducted in e.coli. Fusion of the N-terminal 6xHis tag into the PHOSPHO1 encoding gene fragment was conducted, allowing for easier detection and purification of the PHOSPHO1 protein in subsequent stages.
The human phosphoethanolamine/phosphocholine phosphatase (PHOSPHO1) primarily functions in regulating skeletal mineralization by hydrolyzing phosphocholine and phosphoethanolamine. It is involved in the initiation of hydroxyapatite crystal formation during bone development. In bone and mineralization research, studying PHOSPHO1 contributes to understanding the molecular mechanisms involved in bone mineralization and may have implications for bone-related disorders. PHOSPHO1's involvement in mineralization processes makes it relevant in dental research as well. Investigating PHOSPHO1 provides insights into skeletal biology, mineral homeostasis, and potential applications in developing therapeutic strategies for skeletal disorders, enhancing the understanding of mineralization processes in different physiological and pathological contexts.
