Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

PHOSPHO1

Uniprot No.

Q8TCT1

Research Area

Signal Transduction

Alternative Names

PHOP1_HUMAN; Phosphatase orphan 1; Phospho 1 ; phospho1; Phosphoethanolamine/phosphocholine phosphatase

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-267aa

Target Protein Sequence

MSGCFPVSGLRCLSRDGRMAAQGAPRFLLTFDFDETIVDENSDDSIVRAAPGQRLPESLRATYREGFYNEYMQRVFKYLGEQGVRPRDLSAIYEAIPLSPGMSDLLQFVAKQGACFEVILISDANTFGVESSLRAAGHHSLFRRILSNPSGPDARGLLALRPFHTHSCARCPANMCKHKVLSDYLRERAHDGVHFERLFYVGDGANDFCPMGLLAGGDVAFPRRGYPMHRLIQEAQKAEPSSFRASVVPWETAADVRLHLQQVLKSC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

33.7kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-267 form the expressed segment for recombinant Human PHOSPHO1. The expected molecular weight for the PHOSPHO1 protein is calculated to be 33.7 kDa. Expression of this PHOSPHO1 protein is conducted in e.coli. Fusion of the N-terminal 6xHis tag into the PHOSPHO1 encoding gene fragment was conducted, allowing for easier detection and purification of the PHOSPHO1 protein in subsequent stages.

The human phosphoethanolamine/phosphocholine phosphatase (PHOSPHO1) primarily functions in regulating skeletal mineralization by hydrolyzing phosphocholine and phosphoethanolamine. It is involved in the initiation of hydroxyapatite crystal formation during bone development. In bone and mineralization research, studying PHOSPHO1 contributes to understanding the molecular mechanisms involved in bone mineralization and may have implications for bone-related disorders. PHOSPHO1's involvement in mineralization processes makes it relevant in dental research as well. Investigating PHOSPHO1 provides insights into skeletal biology, mineral homeostasis, and potential applications in developing therapeutic strategies for skeletal disorders, enhancing the understanding of mineralization processes in different physiological and pathological contexts.

Target Background

Function

Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix.

Gene References into Functions

CpG sites located in PHOSPHO1 gene showed differential methylation in association with lipid traits. Genetic variants in PHOSPHO1 gene were also associated with lipid profile. PMID: 28173150
DNA methylation at the PHOSPHO1 locus cg02650017 in blood DNA was associated with a decreased risk for future type 2 diabetes. PMID: 27148772
REVIEW: role of bone-type tissue-nonspecific alkaline phosphatase and PHOSPO1 in vascular calcification PMID: 24533943
Models of PHOSPHO2 and PHOSPHO1 suggest subtle differences in the charge distributions around the putative substrate entry site and in the location of potential H-bond donors. PMID: 16054448
The identification of a novel, alternatively spliced PHOSPHO1 transcript (PHOSPHO1-3a), was described. PMID: 18471996

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Subcellular Location

Extracellular vesicle.

Protein Families

HAD-like hydrolase superfamily, PHOSPHO family

Tissue Specificity

Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.

Database Links

HGNC: 16815

KEGG: hsa:162466

STRING: 9606.ENSP00000406909

UniGene: Hs.405607

Code	CSB-EP819461HU
Abbreviation	Recombinant Human PHOSPHO1 protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Phosphoethanolamine/phosphocholine phosphatase (PHOSPHO1)

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