Recombinant Human Pleckstrin homology-like domain family A member 1 (PHLDA1)

Code CSB-EP845148HU
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
PHLDA1
Uniprot No.
Research Area
Cell Biology
Alternative Names
Apoptosis associated nuclear protein; Apoptosis-associated nuclear protein; DT1P1B11; MGC131738 ; PHLA1_HUMAN; PHLDA1; PHRIP ; Pleckstrin homology like domain family A member 1; Pleckstrin homology-like domain family A member 1; PQ rich protein ; PQ-rich protein; PQR protein; Proline- and glutamine-rich protein; Proline- and histidine-rich protein; T cell death associated gene ; T CELL DEATH ASSOCIATED GENE 51; T-cell death-associated gene 51 protein; Tdag; TDAG51
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-401aa
Target Protein Sequence
MRRAPAAERLLELGFPPRCGRQEPPFPLGVTRGWGRWPIQKRREGARPVPFSERSQEDGRGPAARSSGTLWRIRTRLSLCRDPEPPPPLCLLRVSLLCALRAGGRGSRWGEDGARLLLLPPARAAGNGEAEPSGGPSYAGRMLESSGCKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQLQHQQQQQQQQQQQQQQQPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVDCVERKGKYMYFTVVMAEGKEIDFRCPQDQGWNAEITLQMVQYKNRQAILAVKSTRQKQQHLVQQQPPSQPQPQPQLQPQPQPQPQPQPQPQSQPQPQPQPKPQPQQLHPYPHPHPHPHSHPHSHPHPHPHPHPHQIPHPHPQPHSQPHGHRLLRSTSNSA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
50.5 kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation.
Gene References into Functions
  1. suggest a unique regulatory feature of PHLDA1 to inhibit the ErbB receptor oligomerization process and thereby control the activity of receptor signaling network. PMID: 29233889
  2. Data identified PHLDA1 as a novel p53 target with an ability to repress Akt. PHLDA1 has a so-called split PH domain divided into an N-terminal and a C-terminal portions and seems to be responsible for its localization to the plasma membrane and binding to phosphatidylinositol. In addition, PHLDA1 expression analysis suggests that PHLDA1 has a tumor suppressive function in breast and ovarian cancers. PMID: 30207029
  3. Our study negatively correlates expression of PHLDA1 and Aurora A in IMR-32 cells and sheds new light on functions of PHLDA1 in the neuroblastoma tumor cells, suggesting its role as a pro-apoptotic protein PMID: 27278006
  4. Suggest decreased expression of PHLDA1 may play an important role in tumor progression, and may become a new adjunct biomarker in the prognosis in gastric adenocarcinoma. PMID: 26191222
  5. Data suggest that high PHLDA1 expression is controlled through an ER-NFkappaB-miR-181 regulatory axis and may contribute to a poor clinical outcome in patients with ER+ breast tumors by enhancing stem-like properties in these tumors. PMID: 24954507
  6. PHLDA1 expression is a useful addition in differentiating trichoblastoma and basal cell carcinoma. PMID: 23719479
  7. A role for PHLDA1 as an apoptosis suppressor in oral cancer cells. PMID: 24270013
  8. Data show that downregulation of aurora A kinase by the therapeutic antibody is associated with decreased levels of MYCN protein in cytoplasm, and induced expression of PHLDA1 and P53 proteins. PMID: 23962557
  9. The follicular stem cell marker PHLDA1 (TDAG51) indicates that most basaloid tumors in nevus sebaceus are basal cell carcinomas and not trichoblastomas. PMID: 23489134
  10. PHLDA1 differentiates between desmoplastic trichoepithelioma and morpheaform basal cell carcinoma but shows variable staining in microcystic adnexal carcinoma. PMID: 23398472
  11. the release of Ca(2+) from endoplasmic reticulum stores mediates epithelial-to-mesenchymal transition in human proximal tubular epithelium via the induction of TDAG51 PMID: 22592641
  12. crucial negative regulator and effector of Aurora A kinase in breast cancer PMID: 21807936
  13. PHLDA1 expression marks the putative epithelial stem cells, downregulates ITGA2 and ITGA6, and contributes to intestinal tumorigenesis PMID: 21558389
  14. The progressive loss of PHLDA1 expression in melanomas may play a role in deregulated cell growth and apoptosis resistance in these tumors. PMID: 12384558
  15. expression of TDAG51 in human T-cells does not correlate with activation-induced cell death PMID: 15002043
  16. TDAG51 plays an important role in the anti-apoptotic effects of IGF-I. PMID: 15037619
  17. TDAG51 locus shows an operon-like organization of two head-to-head oriented transcripts that are inversely regulated in T lymphocytes by a CpG-rich bidirectional promoter. PMID: 15315823
  18. Reduced PHLDA1 expression is important in breast cancer progression and could serve as useful prognostic marker of disease outcome. PMID: 17211533
  19. TDAG51 in patients with intractable epilepsy was significantly higher when compared with levels in the controls. PMID: 17870236
  20. The anti-estrogen ICI 182,780 (1 microM) inhibited PHLDA1 mRNA expression and completely abolished the effect of 10 nM 17beta-estradiol on PHLDA1 expression (P < 0.05), suggesting that PHLDA1 is regulated by estrogen via ER. PMID: 18641796

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Subcellular Location
Cytoplasm. Cytoplasmic vesicle. Nucleus, nucleolus.
Tissue Specificity
Widely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level).
Database Links

HGNC: 8933

OMIM: 605335

KEGG: hsa:22822

STRING: 9606.ENSP00000266671

UniGene: Hs.602085

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