Recombinant Human Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1, mitochondrial (DHTKD1), partial

Code CSB-YP842685HU
MSDS
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Source Yeast
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Code CSB-EP842685HU
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Source E.coli
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Code CSB-EP842685HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842685HU
MSDS
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Source Baculovirus
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Code CSB-MP842685HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DHTKD1
Uniprot No.
Alternative Names
AMOXAD; CMT2Q; Dehydrogenase E1 and transketolase domain containing 1; Dehydrogenase E1 and transketolase domain containing protein 1; Dehydrogenase E1 and transketolase domain-containing protein 1; DHTK1_HUMAN; dhtkd1; DKFZp762M115; KIAA1630; MGC3090; Probable 2 oxoglutarate dehydrogenase E1 component DHKTD1 mitochondrial; Probable 2-oxoglutarate dehydrogenase E1 component DHKTD1; mitochondrial
Species
Homo sapiens (Human)
Expression Region
-
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
Gene References into Functions
  1. DHTKD1 encodes the E1 subunit of the alpha-ketoadipic acid dehydrogenase complex PMID: 25860818
  2. DHTKD1 contributes to mitochondrial biogenesis and function maintenance. PMID: 24076469
  3. DHTKD1 mutations cause 2-aminoadipic and 2-oxoadipic aciduria. PMID: 23141293
  4. A nonsense mutation in DHTKD1 causes Charcot-Marie-Tooth disease type 2 in a large Chinese pedigree. PMID: 23141294
Involvement in disease
Charcot-Marie-Tooth disease 2Q (CMT2Q); 2-aminoadipic 2-oxoadipic aciduria (AMOXAD)
Subcellular Location
Mitochondrion.
Protein Families
Alpha-ketoglutarate dehydrogenase family
Database Links

HGNC: 23537

OMIM: 204750

KEGG: hsa:55526

STRING: 9606.ENSP00000263035

UniGene: Hs.104980

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