Recombinant Human Protein MRVI1 (MRVI1), partial

Code CSB-YP896930HU
MSDS
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Source Yeast
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Code CSB-EP896930HU
MSDS
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Source E.coli
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Code CSB-EP896930HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896930HU
MSDS
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Source Baculovirus
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Code CSB-MP896930HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MRVI1
Uniprot No.
Alternative Names
MRVI1; IRAG; JAW1L; Protein MRVI1; Inositol 1,4,5-trisphosphate receptor-associated cGMP kinase substrate; JAW1-related protein MRVI1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role as NO/PRKG1-dependent regulator of IP3-induced calcium release; its phosphorylation by PRKG1 inhibits bradykinin and IP3-induced calcium release from intracellular stores. Recruits PRKG1 to the endoplasmic reticulum and may mediate the assembly of PRKG1 and ITPR1 in a macrocomplex. Involved in PRKG1 signaling cascade leading to inhibition of platelet activation and aggregation. Mediates also NO-dependent inhibition of calcium signaling in gastrointestinal smooth muscle contributing to NO-dependent relaxation.
Gene References into Functions
  1. In the present study we analyzed the structure and function of the human IRAG/MRVI1 gene PMID: 21865585
  2. IRAG is required for PKG1beta-regulated cyclic calcium release during motility of osteoclasts PMID: 20567233
  3. IRAG appears to be essentially involved in the NO/cGK-dependent inhibition of InsP(3)-dependent Ca(2+)-signaling in colonic smooth muscle PMID: 14729908
  4. cGMP-dependent protein kinase Ibeta binds to TFII-I and IRAG through a common interaction motif PMID: 16166082
  5. These findings reveal that interaction between IRAG and InsP3RI has a central role in NO/cGMP-dependent inhibition of platelet aggregation and in vivo thrombosis. PMID: 16990611
  6. Reports use of non-AUG (CUG) translation initiation codon in one of the transcript variants of MRVI1 gene in mouse and human. PMID: 10321731

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Subcellular Location
Cytoplasm, perinuclear region. Sarcoplasmic reticulum. Membrane; Single-pass membrane protein.
Tissue Specificity
Expressed in the colon, rectum, and cultured colonic smooth muscle. Detected in various cancer cell lines.
Database Links

HGNC: 7237

OMIM: 604673

KEGG: hsa:10335

STRING: 9606.ENSP00000412130

UniGene: Hs.501898

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