Recombinant Human Protein TSPEAR (TSPEAR)

Code CSB-YP848817HU
MSDS
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Source Yeast
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Code CSB-EP848817HU
MSDS
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Source E.coli
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Code CSB-EP848817HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP848817HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP848817HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TSPEAR
Uniprot No.
Alternative Names
Thrombospondin-type laminin G domain and EAR repeat-containing protein; TSEAR_HUMAN; TSP-EAR; TSPEAR
Species
Homo sapiens (Human)
Expression Region
20-669
Target Protein Sequence
T QGWEPCTDLR PLDILAEVVP SDGATSGIRI VQVHGARGLQ LSVAAPRTMS FPASRIFSQC DLFPEEFSIV VTLRVPNLPP KRNEYLLTVV AEESDLLLLG LRLSPAQLHF LFLREDTAGA WQTRVSFRSP ALVDGRWHTL VLAVSAGVFS LTTDCGLPVD IMADVPFPAT LSVKGARFFV GSRRRAKGLF MGLVRQLVLL PGSDATPRLC PSRNAPLAVL SIPRVLQALT GKPEDNEVLK YPYETNIRVT LGPQPPCTEV EDAQFWFDAS RKGLYLCVGN EWVSVLAAKE RLDYVEEHQN LSTNSETLGI EVFRIPQVGL FVATANRKAT SAVYKWTEEK FVSYQNIPTH QAQAWRHFTI GKKIFLAVAN FEPDEKGQEF SVIYKWSHRK LKFTPYQSIA THSARDWEAF EVDGEHFLAV ANHREGDNHN IDSVIYKWNP ATRLFEANQT IATSGAYDWE FFSVGPYSFL VVANTFNGTS TKVHSHLYIR LLGSFQLFQS FPTFGAADWE VFQIGERIFL AVANSHSYDV EMQVQNDSYV INSVIYELNV TAQAFVKFQD ILTCSALDWE FFSVGEDYFL VVANSFDGRT FSVNSIIYRW QGYEGFVAVH SLPTVGCRDW EAFSTTAGAY LIYSSAKEPL SRVLRLRTR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a critical role in tooth and hair follicle morphogenesis through regulation of the Notch signaling pathway. May play a role in development or function of the auditory system.
Gene References into Functions
  1. TSPEAR mutation is associated with tooth agenesis. PMID: 30046887
  2. using a luciferase-based reporter assay, we showed that TSPEAR knock-down is associated with decreased Notch signaling. In addition, NOTCH1 protein expression was reduced in patient scalp skin. Moreover, TSPEAR silencing in mouse hair follicle organ cultures was found to induce apoptosis in follicular epithelial cells, resulting in decreased hair bulb diameter PMID: 27736875
  3. TSPEAR expression is significantly downregulated in human masticatory mucosa during wound healing PMID: 28005267
  4. TSPEAR, cause disorders with auditory features: epilepsy, which can include auditory features in humans; audiogenic seizures in animals; and/or hearing impairments in humans and mice. PMID: 22678063
  5. The TSPEAR/C21orf29 promoter is activated by Trichostatin A (TSA) treatment according to promoter reporter assays in HEK 293 cells. PMID: 20494980

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Involvement in disease
Deafness, autosomal recessive, 98 (DFNB98)
Subcellular Location
Secreted. Cell surface. Cell projection, stereocilium.
Database Links

HGNC: 1268

OMIM: 612920

KEGG: hsa:54084

STRING: 9606.ENSP00000321987

UniGene: Hs.660703

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