Recombinant Human Protein maelstrom homolog (MAEL)

Code CSB-YP842695HU
MSDS
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Source Yeast
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Code CSB-EP842695HU
MSDS
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Source E.coli
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Code CSB-EP842695HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842695HU
MSDS
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Source Baculovirus
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Code CSB-MP842695HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MAEL
Uniprot No.
Alternative Names
cancer/testis antigen 128; CT128; FLJ14904; mael; MAEL_HUMAN; maelstrom homolog (Drosophila); Maelstrom homolog; OTTHUMP00000032344; Protein maelstrom homolog; RP11-102C16.1
Species
Homo sapiens (Human)
Expression Region
1-434
Target Protein Sequence
MPNRKASRNA YYFFVQEKIP ELRRRGLPVA RVADAIPYCS SDWALLREEE KEKYAEMARE WRAAQGKDPG PSEKQKPVFT PLRRPGMLVP KQNVSPPDMS ALSLKGDQAL LGGIFYFLNI FSHGELPPHC EQRFLPCEIG CVKYSLQEGI MADFHSFINP GEIPRGFRFH CQAASDSSHK IPISNFERGH NQATVLQNLY RFIHPNPGNW PPIYCKSDDR TRVNWCLKHM AKASEIRQDL QLLTVEDLVV GIYQQKFLKE PSKTWIRSLL DVAMWDYSSN TRCKWHEEND ILFCALAVCK KIAYCISNSL ATLFGIQLTE AHVPLQDYEA SNSVTPKMVV LDAGRYQKLR VGSSGFSHFN SSNEEQRSNT PIGDYPSRAK ISGQNSSVRG RGITRLLESI SNSSSNIHKF SNCDTSLSPY MSQKDGYKSF SSLS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for the localization of germ-cell factors to the meiotic nuage.
Gene References into Functions
  1. MAEL promoter hypermethylation may have a role in de-repression of LINE-1 in human hypospermatogenesis PMID: 29095993
  2. Report found that Mael is activated in proliferating cancer cells, where it serves to maintain genetic stability. Its depletion results in an increase in reactive oxygen species, followed by apoptosis or senescence. PMID: 27926513
  3. Data suggest that overexpression of MAEL, caused by gene amplification and/or decreased miR-186, has a critical oncogenic role in UCB pathogenesis by downregulation of MTSS1. PMID: 27181205
  4. Study demonstrated that MAEL interacts with Snail and inhibit E-cadherin promoter activity. MAEL is an oncogene that plays an important role in the development and progression of colon cancer. PMID: 27537253
  5. Immunofluorescence analysis showed that MAEL co-localizes with the stress granule marker PABPC1 in stress granules during oxidative stress. MAEL may be involved in miRNA-mediated gene silencing in SGs. PMID: 24189637
  6. Maelstrom promotes hepatocellular carcinoma metastasis by inducing epithelial-mesenchymal transition by way of Akt/GSK-3beta/Snail signaling. PMID: 23929794
  7. These results suggest that MAEL is a novel cancer/testis-associated gene and is regulated by DNA methylation. PMID: 19693694

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Maelstrom family
Tissue Specificity
Testis-specific. Expressed in various cancer cell lines, probably due to demethylation of its promoter.
Database Links

HGNC: 25929

OMIM: 611368

KEGG: hsa:84944

STRING: 9606.ENSP00000356846

UniGene: Hs.651245

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