Recombinant Human Protocadherin Fat 1 (FAT1), partial

In Stock
Code CSB-EP614532HU
Abbreviation Recombinant Human FAT1 protein, partial
MSDS
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Others
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
4203-4588aa
Target Protein Sequence
RKMISRKKKHQAEPKDKHLGPATAFLQRPYFDSKLNKNIYSDIPPQVPVRPISYTPSIPSDSRNNLDRNSFEGSAIPEHPEFSTFNPESVHGHRKAVAVCSVAPNLPPPPPSNSPSDSDSIQKPSWDFDYDTKVVDLDPCLSKKPLEEKPSQPYSARESLSEVQSLSSFQSESCDDNGYHWDTSDWMPSVPLPDIQEFPNYEVIDEQTPLYSADPNAIDTDYYPGGYDIESDFPPPPEDFPAADELPPLPPEFSNQFESIHPPRDMPAAGSLGSSSRNRQRFNLNQYLPNFYPLDMSEPQTKGTGENSTCREPHAPYPPGYQRHFEAPAVESMPMSVYASTASCSDVSACCEVESEVMMSDYESGDDGHFEEVTIPPLDSQQHTEV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
49.2 kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays an essential role for cellular polarization, directed cell migration and modulating cell-cell contact.
Gene References into Functions
  1. Fat1 functional loss results in YAP1 activation and is associated with human malignancies. PMID: 29985391
  2. Study showed that FAT1 exhibits a high frequency of mutations and a downregulated expression in esophageal squamous cell carcinoma (ESCC) leading to cell migration and invasion by affecting the cellular mechanical properties of ESCC cells. PMID: 29565465
  3. FAT1 has a novel regulatory effect on EMT/stemness markers both via or independent of HIF-1alpha. The functional relevance of this study was corroborated by significant reduction in the number of soft-agar colonies formed in hypoxic-siFAT1 treated U87MG cells. PMID: 28994107
  4. FAT1 mutational status is a strong independent prognostic factor in patients with HPV-negative head and neck squamous cell carcinoma; FAT1 mutation was significantly associated with better overall survival PMID: 26876381
  5. Disruption of MAPK/ERK pathway by FAT1 contributes the epithelial mesenchymal transformation in esophageal squamous cell carcinomas. PMID: 28366557
  6. Loss of function mutations in FAT1 and CASP8 prevent cell adhesion and promote cell migration and proliferation in oral squamous cell carcinoma cell lines. PMID: 27693639
  7. We identified recurrent mutations in the novel penile cancer tumor suppressor genes CSN1(GPS1) and FAT1 PMID: 27325650
  8. FAT1 and mAb198.3 may offer new therapeutic opportunities for CRC. PMID: 27328312
  9. Data show that the two N-terminal SH3 domains of SH3 domain containing ring finger 1 (SH3RF1) protein interact with FAT1 protein. PMID: 28129444
  10. At the molecular level, under hypoxia the FAT1 depletion-associated reduction in HIF1alpha was due to compromised EGFR-Akt signaling as well as increased VHL-dependent proteasomal degradation of HIF1alpha. PMID: 27536856
  11. Low FAT1 expression was associated with poor prognosis in children with medulloblastoma. Furthermore, FAT1 may act on Wnt signaling pathway to exert its antitumor effect PMID: 27834469
  12. Recessive mutations in FAT1 cause a distinct renal disease entity in four families with a combination of steroid-resistance nephrotic syndrome, tubular ectasia, haematuria and facultative neurological involvement. PMID: 26905694
  13. that loss of FAT1 and beta-catenin are associated with breast cancer progression, aggressive behavior, and poor prognosis PMID: 26721716
  14. FAT1 protein acts upstream of Hippo signalling through TAZ protein to regulate neuronal differentiation. PMID: 26104008
  15. Fat1 is released from pancreatic cancer cells in its soluble form by ADAM10 mediated ectodomain shedding PMID: 24625754
  16. our data suggest that defective FAT1 is associated with an FSHD-like phenotype. PMID: 25615407
  17. FAT1 is expressed at lower levels in muscles that are affected at early stages of facioscapulohumeral muscular dystrophy progression. PMID: 26018399
  18. Analysis revealed an aberrant expression of FAT1 predominantly in mature BCP-ALL and thymic T-ALL and a high rate of FAT1 mutations. PMID: 24972153
  19. FJX1 does not influence the levels of FAT1 ectodomain phosphorylation. PMID: 25150169
  20. FAT1 expression in HCC is regulated via promotor methylation. PMID: 24590895
  21. Fat1 may therefore provide a new marker of MRD for patients with ALL lacking known genomic aberrations or within a multiplex approach to MRD detection. PMID: 23433465
  22. This work establishes S1-processing as a clear functional prerequisite for ectodomain shedding of FAT1 with general implications for the shedding of other transmembrane receptors. PMID: 24560745
  23. frequency of FAT1-mutated cases was significantly higher in drug resistant chronic lymphocytic leukemia than in unselected chronic lymphocytic leukemia PMID: 24550227
  24. There is a mechanism to regulate death receptor-mediated apoptosis via an interaction between FAT1 and procaspase-8. PMID: 24442637
  25. this study identifies a novel signaling mechanism mediated by FAT1 in regulating the activity of PDCD4 in gliomas. PMID: 22986533
  26. Our study identifies FAT1 as a critical determinant of muscle form, misregulation of which associates with facioscapulohumeral dystrophy . PMID: 23785297
  27. Taken together, these data strongly point to FAT1 as a tumor suppressor gene driving loss of chromosome 4q35, a prevalent region of deletion in cancer PMID: 23354438
  28. FAT1 suppression in activated hepatic stellate cells caused a downregulation of NFkappaB activity PMID: 22959770
  29. Lipid accumulation in myotubes derived from obese type 2 diabetic patients arises from abnormal FAT/CD36 cycling. PMID: 22194967
  30. data presented demonstrate that Fat1 expression in preB-ALL has a role in the emergence of relapse and could provide a suitable therapeutic target in high-risk preB-ALL PMID: 22116550
  31. In vitro localization studies of FAT1 showed that melanoma cells display high levels of cytosolic FAT1 protein, whereas keratinocytes, despite comparable FAT1 expression levels, exhibited mainly cell-cell junctional staining PMID: 21680732
  32. FAT1 may be involved in the migration and invasion mechanisms of oral squamous cell carcinoma cells PMID: 21617878
  33. Processing of FAT1 and the translocation of its cytoplasmic domain to the nucleus were studied. PMID: 15922730
  34. A cadherin gene, FAT, confers susceptibility to bipolar disorder in four independent cohorts. In mice, Fat was shown to be significantly downregulated and Catnb and Enah were significantly upregulated in response to therapeutic doses of lithium. PMID: 16402135
  35. Fat1 exhibit co-localisation with Homer-3 in cellular protrusions and at the plasma membrane of HeLa cells PMID: 16979624
  36. results identify mutations in FAT as an important factor in the development of oral cancer and indicate the importance of FATs function in some squamous cell carcinomas PMID: 17325662
  37. FAT1(WT) is up-regulated in migration, induces cellular process formation when overexpressed, and is necessary for efficient wound healing PMID: 17500054
  38. This study did not support the two FAT polymorphism in the affectve disorder. PMID: 17895925
  39. The results of this study supports an involvement of variation at the FAT gene in the etiology of BPAD. PMID: 17938632
  40. Results point to a role of the FAT in astrocytic tumorigenesis and demonstrate the use of RAPD analysis in identifying specific alterations in astrocytic tumors. PMID: 19126244

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Subcellular Location
[Protocadherin Fat 1]: Cell membrane; Single-pass type I membrane protein.; [Protocadherin Fat 1, nuclear form]: Nucleus.
Tissue Specificity
Expressed in many epithelial and some endothelial and smooth muscle cells.
Database Links

HGNC: 3595

OMIM: 600976

KEGG: hsa:2195

STRING: 9606.ENSP00000406229

UniGene: Hs.481371

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