Recombinant Human Putative ribosomal RNA methyltransferase 1 (FTSJ1)

Code CSB-YP866214HU
MSDS
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Source Yeast
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Code CSB-EP866214HU
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Source E.coli
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Code CSB-EP866214HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866214HU
MSDS
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Source Baculovirus
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Code CSB-MP866214HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FTSJ1
Uniprot No.
Alternative Names
CDLIV; FTSJ 1; FtsJ homolog 1 (E. coli); FtsJ homolog 1; FtsJ RNA methyltransferase homolog 1; FTSJ1; JM23; Mental retardation X linked 44; Mental retardation X linked 9; MRX44 ; MRX9; Protein ftsJ homolog 1; Putative ribosomal RNA methyltransferase 1; RRMJ1; RRMJ1_HUMAN; rRNA (uridine 2' O ) methyltransferase ; rRNA (uridine-2''-O-)-methyltransferase; SPB1; TRM7
Species
Homo sapiens (Human)
Expression Region
1-329
Target Protein Sequence
MGRTSKDKRD VYYRLAKENG WRARSAFKLL QLDKEFQLFQ GVTRAVDLCA APGSWSQVLS QKIGGQGSGH VVAVDLQAMA PLPGVVQIQG DITQLSTAKE IIQHFKGCPA DLVVCDGAPD VTGLHDVDEY MQAQLLLAAL NIATHVLKPG GCFVAKIFRG RDVTLLYSQL QVFFSSVLCA KPRSSRNSSI EAFAVCQGYD PPEGFIPDLS KPLLDHSYDP DFNQLDGPTR IIVPFVTCGD LSSYDSDRSY PLDLEGGSEY KYTPPTQPPI SPPYQEACTL KRKGQLAKEI RPQDCPISRV DTFPQPLAAP QCHTLLAPEM EDNEMSCSP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.
Gene References into Functions
  1. we have provided strong evidence that human FTSJ1 is required for Cm32 and Gm34 modification of tRNAPhe. PMID: 26310293
  2. May play a role in the regulation of translation. Mutations cause X-linked mental retardation. PMID: 15162322
  3. A splice site mutation is associated with non-syndromic mental retardation in a large Belgian family PMID: 15342698
  4. A 50kb deletion at Xp11.23 including the two genes, SLC38A5 and FTSJ1 was found in 3 brothers with moderate to severe mental retardation. PMID: 17333282
  5. identified a novel FTSJ1 mutation in an X-linked mental retardation family through mutation screening of a cohort of 73 unrelated Japanese male probands with MR PMID: 18081026
  6. Results suggest a positive association between genetic variants and nonsyndromic X-linked mental retardation in young male subjects in the Chinese Han population. PMID: 18401546
  7. These findings suggest that genetic variations in FtsJ homolog 1 (E. coli) possibly influence human cognitive ability. PMID: 19012053
  8. Spb1p is a homologous yeast nucleolar protein PMID: 10648622

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Involvement in disease
Mental retardation, X-linked 44 (MRX44)
Subcellular Location
Cytoplasm.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, RNA methyltransferase RlmE family, TRM7 subfamily
Tissue Specificity
Found in fetal brain, lung, liver and kidney. In the adult brain, expressed in amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus.
Database Links

HGNC: 13254

OMIM: 300499

KEGG: hsa:24140

STRING: 9606.ENSP00000326948

UniGene: Hs.23170

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