Recombinant Human Ras-related protein Rab-32 (RAB32)

Code CSB-YP618795HU
MSDS
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Source Yeast
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Code CSB-EP618795HU
MSDS
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Source E.coli
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Code CSB-EP618795HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP618795HU
MSDS
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Source Baculovirus
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Code CSB-MP618795HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAB32
Uniprot No.
Alternative Names
RAB32; RAB32, member RAS oncogene family; RAB32_HUMAN; Ras related protein Rab32; Ras-related protein Rab-32
Species
Homo sapiens (Human)
Expression Region
2-225
Target Protein Sequence
AGGGAGDPG LGAAAAPAPE TREHLFKVLV IGELGVGKTS IIKRYVHQLF SQHYRATIGV DFALKVLNWD SRTLVRLQLW DIAGQERFGN MTRVYYKEAV GAFVVFDISR SSTFEAVLKW KSDLDSKVHL PNGSPIPAVL LANKCDQNKD SSQSPSQVDQ FCKEHGFAGW FETSAKDNIN IEEAARFLVE KILVNHQSFP NEENDVDKIK LDQETLRAEN KSQCC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays an important role in the control of melanin production and melanosome biogenesis. In concert with RAB38, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes.
Gene References into Functions
  1. megalin is critical for mitochondrial biology; mitochondrial intracrine signaling is a continuum of the retrograde early endosome-to-Golgi-Rab32 pathway and defects in this pathway may underlie disease processes in many systems. PMID: 29916093
  2. this study provides some compelling evidence showing that Rab32 is an essential host factor required for HCV particle assembly and thus that Rab32 could be a potential therapeutic target to interrupt HCV propagation. PMID: 27852857
  3. Rab32 controls intracellular lipid accumulation through inducing lipolysis via enhancing ATGL expression indirectly. PMID: 26882978
  4. Rab32 may regulate the physiological functions of LRRK2. PMID: 25360523
  5. Rab38 and its close homolog Rab32 bind to Myosin Vc but not to Myosin Va or Myosin Vb. Binding depends on residues in the switch II region of Rab32 and Rab38 and regions of the Myosin Vc coiled-coil tail domain. PMID: 25324551
  6. BLOC-3 is a Rab32 and Rab38 guanine nucleotide exchange factor, with a specific function in the biogenesis of lysosome-related organelles. PMID: 23084991
  7. that Rab38 and Rab32 are the specific factors that direct the ubiquitous machinery to mediate transport from early endosomes to maturing LROs. PMID: 22511774
  8. Identification of two new loci at IL23R and RAB32 that influence susceptibility to leprosy PMID: 22019778
  9. Rab32 is a dual function protein that participates in both mitochondrial anchoring of PKA and synchronization of mitochondrial fission. PMID: 12186851
  10. RAB32 inactivation may represent a component of the oncogenic pathway of microsatellite-unstable gastrointestinal adenocarcinomas PMID: 16557577
  11. Results suggest that Rab32 facilitates the formation of autophagic vacuoles whose membranes are derived from the ER and regulates the clearance of aggregated proteins by autophagy. PMID: 19593531

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Subcellular Location
Mitochondrion. Mitochondrion outer membrane; Lipid-anchor. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Melanosome membrane.
Protein Families
Small GTPase superfamily, Rab family
Tissue Specificity
Widely expressed with high levels in heart, liver, kidney, bone marrow, testis, colon and fetal lung.
Database Links

HGNC: 9772

OMIM: 612906

KEGG: hsa:10981

STRING: 9606.ENSP00000356465

UniGene: Hs.287714

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