Recombinant Human Retinal dehydrogenase 1(ALDH1A1)

Code CSB-YP001565HU
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Source Yeast
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Code CSB-EP001565HU
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Source E.coli
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Code CSB-EP001565HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001565HU
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Source Baculovirus
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Code CSB-MP001565HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ALDH1A1
Uniprot No. P00352
Alternative Names Acetaldehyde dehydrogenase 1; AHD2; AL1A1_HUMAN; ALDC; Aldehyde dehydrogenase 1 family member A1; Aldehyde dehydrogenase 1 soluble; Aldehyde dehydrogenase 1A1; Aldehyde dehydrogenase; Aldehyde dehydrogenase cytosolic; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase liver cytosolic; ALDH 1; ALDH 1A1; ALDH class 1; ALDH; liver cytosolic; ALDH-E1; ALDH1 A1; ALDH1; ALDH11; ALDH1A1; ALHDII; cytosolic; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; HEL-S-53e; MGC2318; PUMB1; RALDH 1; RalDH1; Retinal dehydrogenase 1
Species Homo sapiens (Human)
Expression Region 2-501
Target Protein Sequence SSSGTPDLP VLLTDLKIQY TKIFINNEWH DSVSGKKFPV FNPATEEELC QVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLY KLADLIERDR LLLATMESMN GGKLYSNAYL NDLAGCIKTL RYCAGWADKI QGRTIPIDGN FFTYTRHEPI GVCGQIIPWN FPLVMLIWKI GPALSCGNTV VVKPAEQTPL TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDID KVAFTGSTEV GKLIKEAAGK SNLKRVTLEL GGKSPCIVLA DADLDNAVEF AHHGVFYHQG QCCIAASRIF VEESIYDEFV RRSVERAKKY ILGNPLTPGV TQGPQIDKEQ YDKILDLIES GKKEGAKLEC GGGPWGNKGY FVQPTVFSNV TDEMRIAKEE IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GVFTKDIDKA ITISSALQAG TVWVNCYGVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTVKISQKN S
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid. Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid. This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic. Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification. Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins. Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission.
Gene References into Functions
  1. ALDH expression in decidual mesenchymal stem cells is required for cellular resistance to oxidative stress. PMID: 28205523
  2. NGFR and ALDH1A1+ cells are lost during tumourigenesis PMID: 29891189
  3. These data revealed crucial roles of ALDH(-) cancer stem cell in Papillary thyroid carcinoma biology and suggested that cancer stem cell subpopulations function cooperatively to control Papillary thyroid carcinoma initiation and progression. PMID: 29274322
  4. ALDH1 and tumor infiltrating lymphocytes as predictors for neoadjuvant chemotherapy response in breast cancer PMID: 29685462
  5. the expression of the ALDH1A1 protein in colorectal cancer (CRC) tissues was significantly associated with the presence of lymph node metastases and might be a potential prognostic marker in patients with CRC. PMID: 29748529
  6. High ALDH1A1 expression may be a prognostic indicator of survival in patients with gastric neuroendocrine carcinoma PMID: 29103772
  7. In this review, we elevated the role of ALDH to be a potential marker to define and distinguish hematopoietic stem cells and leukemia stem cells and its importance in prognosis and target therapy in AML patients PMID: 29516013
  8. Quercetin suppressed breast cancer stem cell proliferation, self-renewal, and invasiveness. It also lowered the expression levels of proteins related to tumorigenesis and cancer progression, such as aldehyde dehydrogenase 1A1, C-X-C chemokine receptor type 4, mucin 1, and epithelial cell adhesion molecules. PMID: 29353288
  9. Basing on the available evidence, this meta-analysis showed that high levels of ALDH1 expression correlate with worse OS and PFS in ovarian cancer patients. PMID: 29753392
  10. Data suggested that ALDH1A1 may be an independent prognostic indicator and a new molecular biomarker for diagnosis in B-cell non-Hodgkin's lymphoma. PMID: 29399699
  11. findings suggested that ALDH1 played an important role in treatment response and the tumor-promoting microenvironment in OSCC PMID: 28939082
  12. NFATc2 enhances tumor-initiating phenotypes through the NFATc2/SOX2/ ALDH1A1 axis in lung adenocarcinoma. PMID: 28737489
  13. significant association between ALDH1A1 expressions with Gleason score indicates the potential role of this protein in PCa tumorigenesis and aggressive behavior; therefore, this cancer stem cell marker can be used as a promising candidate for targeted therapy of PCa, especially those with high Gleason score PMID: 26894647
  14. this study identified TAZ as a novel inducer of lung cancer stem cells and the first transcriptional activator of the stem cell marker ALDH1A1 PMID: 28415606
  15. these findings indicate that ALDH1 can be prognosis prediction markers for urinary tract carcinomas PMID: 28418868
  16. Circulating tumor cells expressing cytokeratin and tumor-initiating cell markers, including ALDH, CD133, and CD44, were identified in patients with pancreatic adenocarcinoma. These TIC-like CTCs were associated with poor prognosis after surgical resection and with an increased incidence of tumor recurrence PMID: 27789528
  17. approximately 25% of all acute myeloid leukemias expressed low or undetectable levels of ALDH1A1 and this ALDH1A1(-) subset of leukemias correlates with good prognosis cytogenetics PMID: 28280079
  18. These findings suggest that ALDH1 expression in malignant melanoma has a favorable effect on patient survival PMID: 28106104
  19. disulfiram/copper complex targets ALDH1A1 to inhibit non-small cell lung cancer recurrence driven by ALDH-positive cancer stem cells PMID: 27542268
  20. Mesenchymal stem cells selected for ALDH(hi) demonstrated enhanced proangiogenic secretory functions and represent a purified MSC subset amenable for vascular regenerative applications. PMID: 28295901
  21. ALDH appears to be involved in the interaction between lung and Osteosarcoma (OS) cells, and ALP may be a valuable biomarker for monitoring functional OS changes during metastasis. PMID: 28122543
  22. CD44 and ALDH are the most specific biomarkers to detect and isolate tumorigenic and chemoresistant gastric CSCs in noncardia gastric carcinomas independently of the histologic classification of the tumor. PMID: 27620279
  23. Low expression level of ALDH1A1 is associated with prostate cancer. PMID: 26934553
  24. Collectively, this study illustrates the interplay between ALDH(high) CSCLs and EOC invasion, and offers a novel application target for the EOC oncotherapy. PMID: 29170132
  25. Findigs suggest that aldehyde dehydrogenase 1 (ALDH1) played an important role in tumor aggressiveness and associated with a tumor-promoting microenvironment in esophageal cancer. PMID: 28888039
  26. These results suggested that tumor-secreting factor increases ALDH(high) TEC populations that are resistant to 5-FU. Therefore, ALDH(high) TEC in tumor blood vessels might be an important target to overcome or prevent drug resistance. PMID: 28851003
  27. Results demonstrate that EZH2 and ALDH1 proteins are expressed in the stromal component of phyllodes tumors. PMID: 27290698
  28. High ALDH1A1 expression is associated with cervical cancer. PMID: 28449663
  29. Study shows that BPA induces ALDH1 expression in MCF-7 Cells and increases mammosphere size. PMID: 28244015
  30. High ALDH expression is associated with Prostate Cancer. PMID: 28870918
  31. High ALDH1 expression is associated with endometrial cancer. PMID: 28604744
  32. A central role of Aurora kinase A (AURKA) in promoting Epithelial-to-mesenchymal transition and cancer stem cell phenotypes via ALDH1A1. PMID: 28193222
  33. ALDH1A1 modulates the production of ROS by anti-cancer agents such as paclitaxel and diminishes their efficacy. PMID: 28756208
  34. High expression of ALDH1 is associated with non-small cell lung cancer. PMID: 27832750
  35. NOR1 suppresses cancer stem-like cell properties in nasopharyngeal carcinoma cells by inhibiting the AKT-GSK-3beta-Wnt/beta-catenin-ALDH1A1 signaling circuit. PMID: 27891591
  36. FBLN-3 promoter region was highly methylated in some pancreatic cancer cell lines. FBLN-3 inhibited c-MET activation and expression and reduced cellular level of ALDH1. PMID: 25451256
  37. ALDH1A1 silencing induced sensitivity in the cisplatin resistant head and neck cancer cell line In Vitro. PMID: 27380877
  38. Among patients with ER-positive/HER2-negative breast cancer, ALDH1 expression was more common in those with early recurrence, and this expression was found to be associated with a more aggressive breast cancer phenotype than that in the patients without recurrence. PMID: 27368476
  39. We demonstrate the utility of this assay by generating dose-response curves on a comprehensive set of prior art inhibitors as well as hundreds of ALDH1A1 inhibitors synthesized in house. Finally, we established a screening paradigm using a pair of cell lines with low and high ALDH1A1 expression, respectively, to uncover novel cell-active ALDH1A1-specific inhibitors from a collection of over 1,000 small molecules. PMID: 28129349
  40. The expression of CD133, but not ALDHA1A, was significantly associated with poorer overall survival (p<0.0001) and shorter disease-free interval (DFI) (p<0.0001) in lung adenocarcinoma PMID: 28476825
  41. Results suggest that ALDH1 expression correlates with aggressive phenotypes, and that high ALDH1 expression predicts a poor outcome in breast cancer. PMID: 28640095
  42. ALDH1A1 and IGFBP1 are differentially overexpressed in CLM and may play a dual role, functioning as both tumor suppressors and metastasis promoters in CRC PMID: 27152521
  43. CD44+/24- and ALDH1-positive rates in primary tumors differed according to intrinsic subtype. ER-positive patients with CD44+/24- tumors had significantly longer disease-free-survival than all other ER-positive patients PMID: 27768764
  44. prognostic indicator of survival in triple negative breast cancer PMID: 28383433
  45. ALDH1 positivity is associated with poor prognosis of cervical carcinoma, and may be an independent predictor of prognosis. PMID: 24827824
  46. High ALDH1A1 expression is associated with bladder cancer. PMID: 28184944
  47. Results indicate that ALDH1A1 is associated with cisplatin resistance in human lung adenocarcinoma cell line A549/DDP cells, and that activation of the PI3K/AKT signal pathway may be the responsible mechanism. PMID: 27813328
  48. We ascertained the presence of strong association between ALDH1A1 expression and both histological subtype & tumor grade of non-small cell lung cancers. We report a fascinating finding; ALDH1A1 heterogeneity within adenocarcinoma can be mapped to its histological patterns. PMID: 26907783
  49. Whole-tissue is better than TMA for the detection of ALDH1A1 nuclear staining in colorectal carcinoma patients, and nuclear expression is associated with a better outcome. Cytoplasmic expression is not a suitable prognostic marker of colorectal carcinoma. PMID: 27461829
  50. Data support a crucial role of Notch3 in the increase of stem-like property in NSCLC cells that might be associated with upregulation of ALDH1A1 and CD44 and activation of autophagy. PMID: 27035162

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Subcellular Location Cytoplasm, cytosol. Cell projection, axon.
Protein Families Aldehyde dehydrogenase family
Tissue Specificity Expressed by erythrocytes (at protein level).
Database Links

HGNC: 402

OMIM: 100640

KEGG: hsa:216

STRING: 9606.ENSP00000297785

UniGene: Hs.76392

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