Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

RBKS

Uniprot No.

Q9H477

Research Area

Metabolism

Alternative Names

RBKS; RBSK; Ribokinase; RK; EC 2.7.1.15

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-322aa

Target Protein Sequence

AASGEPQRQWQEEVAAVVVVGSCMTDLVSLTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSFGNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLLLNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAPAIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQVVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLAYYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

50.0kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 2-322 constitute the expression domain of recombinant Human RBKS. This RBKS protein is theoretically predicted to have a molecular weight of 50 kDa. This RBKS protein is produced using e.coli expression system. The RBKS coding gene included the N-terminal 6xHis-SUMO tag, which simplifies the detection and purification processes of the recombinant RBKS protein in following stages of expression and purification.

Ribokinase (RBKS) is a crucial enzyme involved in the phosphorylation of ribose, playing a central role in the cellular metabolism of carbohydrates. As part of the pentose phosphate pathway, RBKS catalyzes the conversion of ribose to ribose-5-phosphate by transferring a phosphate group from ATP to ribose. This phosphorylation step is vital for the subsequent utilization of ribose-5-phosphate in various metabolic pathways, including nucleotide biosynthesis and the generation of reducing equivalents. Beyond its central role in energy metabolism, RBKS has been implicated in cellular stress responses and is associated with the pathogenesis of certain diseases. Investigating RBKS provides insights into the intricate regulation of cellular processes and its potential implications in metabolic disorders.

Target Background

Function

Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.

Gene References into Functions

Asn199 and Glu202 play an important role in the regulation of human ribokinase activity. PMID: 25749547
Identification and characterization of human ribokinase. PMID: 17585908

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Carbohydrate kinase PfkB family, Ribokinase subfamily

Database Links

HGNC: 30325

OMIM: 611132

KEGG: hsa:64080

STRING: 9606.ENSP00000306817

UniGene: Hs.11916

Code	CSB-EP019397HU
Abbreviation	Recombinant Human RBKS protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Ribokinase (RBKS), partial

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