Recombinant Human Semaphorin-4C (SEMA4C), partial

Code CSB-YP863177HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP863177HU
MSDS
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Source E.coli
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Code CSB-EP863177HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863177HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP863177HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SEMA4C
Uniprot No.
Alternative Names
KIAA1739; M-SEMA-F; S4c; SEM4C_HUMAN; Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C; SEMA4C; SEMACL1; SEMAF; SEMAI; Semaphorin-4C; UNQ5855/PRO34487
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.
Gene References into Functions
  1. Tumor-associated LECs produce sSEMA4C to promote lymphatic metastasis of tumors. PMID: 27401250
  2. ectopic expression of miR-25-3p reversed the epithelial-mesenchymal transition phenotype and sensitized cisplatin-resistant cervical cancer cells to cisplatin via targeting Sema4C PMID: 27743413
  3. Data show that G-protein-coupled receptor kinase-interacting protein 1 (GIT1) and semaphorin 4C (SEMA4C) were found to have putative microRNA miR-138 binding sites in their 3' untranslated region (3'UTRs). PMID: 26283050
  4. Suggest that up-regulation of miR-125b or targeting Sema4C could serve as novel approaches to reverse chemotherapy resistance in breast cancers. PMID: 25605244
  5. Data suggest that Erbin can interact with Sema4C, and co-expression of Erbin blocks the process of Sema4C-induced epithelial-mesenchymal transition. PMID: 24142719
  6. There is a high expression of Sema4C in esophageal cancer, gastric cancer and rectal cancer, and expression is strongly correlated with lymphatic metastasis. PMID: 22944267
  7. High Sema4C is associated with epithelial-mesenchymal transition and renal fibrosis. PMID: 20959347

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Subcellular Location
Cell junction, synapse, postsynaptic density membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type I membrane protein.
Protein Families
Semaphorin family
Database Links

HGNC: 10731

OMIM: 604462

KEGG: hsa:54910

STRING: 9606.ENSP00000306844

UniGene: Hs.516220

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