Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cardiovascular
Species
Homo sapiens (Human)
Expression Region
804-916aa
Target Protein Sequence
CGGELGEFTGYIESPNYPGNYPAGVECIWNINPPPKRKILIVVPEIFLPSEDECGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSRKLWINFKTSEANSARGFQIPYVTY
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 804-916 constitute the expression domain of recombinant Human SCUBE3. The expected molecular weight for the SCUBE3 protein is calculated to be 20.2 kDa. This protein is generated in a e.coli-based system. The SCUBE3 coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant SCUBE3 protein in following stages of expression and purification.
Signal peptide, CUB, and EGF-like domain-containing protein 3 (SCUBE3) contains a signal peptide for secretion, as well as CUB (Complement C1r/C1s, Uegf, Bmp1) and EGF-like domains, suggesting a role in cell adhesion and signaling. SCUBE3 has been implicated in angiogenesis, where it interacts with vascular endothelial growth factor (VEGF) and modulates VEGF-mediated signaling pathways. Additionally, it has been associated with platelet function, playing a role in platelet aggregation and adhesion.
