Recombinant Human Smad nuclear-interacting protein 1 (SNIP1)

Code CSB-YP844702HU
MSDS
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Source Yeast
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Code CSB-EP844702HU
MSDS
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Source E.coli
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Code CSB-EP844702HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844702HU
MSDS
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Source Baculovirus
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Code CSB-MP844702HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SNIP1
Uniprot No.
Alternative Names
FHA domain containing protein SNIP1; FHA domain-containing protein SNIP1; FLJ12553; PMRED; Smad nuclear interacting protein (Smad nuclear interacting); smad nuclear interacting protein 1; Smad nuclear interacting protein; Smad nuclear-interacting protein 1; SNIP1 (Smad nuclear interacting protein); SNIP1; SNIP1_HUMAN; Splicing factor arginine/serine rich 4 (Pre mRNA splicing factor SRP75)
Species
Homo sapiens (Human)
Expression Region
1-396
Target Protein Sequence
MKAVKSERER GSRRRHRDGD VVLPAGVVVK QERLSPEVAP PAHRRPDHSG GSPSPPTSEP ARSGHRGNRA RGVSRSPPKK KNKASGRRSK SPRSKRNRSP HHSTVKVKQE REDHPRRGRE DRQHREPSEQ EHRRARNSDR DRHRGHSHQR RTSNERPGSG QGQGRDRDTQ NLQAQEEERE FYNARRREHR QRNDVGGGGS ESQELVPRPG GNNKEKEVPA KEKPSFELSG ALLEDTNTFR GVVIKYSEPP EARIPKKRWR LYPFKNDEVL PVMYIHRQSA YLLGRHRRIA DIPIDHPSCS KQHAVFQYRL VEYTRADGTV GRRVKPYIID LGSGNGTFLN NKRIEPQRYY ELKEKDVLKF GFSSREYVLL HESSDTSEID RKDDEDEEEE EEVSDS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for pre-mRNA splicing as component of the spliceosome. Down-regulates NF-kappa-B signaling by competing with RELA for CREBBP/EP300 binding. Involved in the microRNA (miRNA) biogenesis. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
Gene References into Functions
  1. these data demonstrated a novel mechanism whereby SNIP1 regulates intestinal inflammation through modulating intestinal epithelial barrier function PMID: 29426045
  2. SUMOylation of SNIP1 leads to the loss of SNIP1-mediated inhibition on expression of the TGF-beta target genes PAI-1 and MMP2 and eventually enhances TGF-beta-regulated cell migration and invasion. PMID: 27703003
  3. Involvement of SNIP1 in progression of lung cancer by regulating the RB/HDAC1 interaction. PMID: 23932364
  4. SNIP1 in cells strongly activated the heat shock element signaling pathway, and SNIP1 might selectively regulate the transcription of some heat shock proteins through associating with heat shock elements PMID: 22020748
  5. Study identified sequence variants in the known disease-causing genes SLC6A3 and FLVCR1, and present evidence to strongly support the pathogenicity of variants identified in TUBGCP6, BRAT1, SNIP1, CRADD, and HARS. PMID: 22279524
  6. overexpression of HIF-1alpha, TWIST2 or SNIP1 correlated with poor disease-free survival in patients with tongue squamous cell carcinoma PMID: 21167768
  7. define both a new function for SNIP1 and identify a previously unrecognized regulator of the cell cycle and cyclin D1 expression PMID: 15378006
  8. Smad-binding peptide aptamers can be developed to selectively inhibit TGF-beta-induced gene expression. PMID: 15750622
  9. SNIP1 functions as a regulator of c-Myc activity, binding the N c-Myc N terminus through its C terminus, and that SNIP1 enhances the transcriptional activity of c-Myc by stabilizing it against proteosomal degradation and bridging the c-Myc/p300 complex. PMID: 17157259
  10. SNIP1, the human homolog of DDL, is involved in miRNA biogenesis and interacts with Drosha PMID: 18632581
  11. Study shows a novel mechanism regulating Cyclin D1 expression and offers new insight into the role of SNIP1 and associated proteins as regulators of proliferation and cancer. PMID: 18794151

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Involvement in disease
Psychomotor retardation, epilepsy, and craniofacial dysmorphism (PMRED)
Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitous, with highest expression in heart and skeletal muscle.
Database Links

HGNC: 30587

OMIM: 608241

KEGG: hsa:79753

STRING: 9606.ENSP00000296215

UniGene: Hs.47232

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