Recombinant Human Spermatogenesis-associated protein 16 (SPATA16)

Code CSB-YP871597HU
MSDS
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Source Yeast
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Code CSB-EP871597HU
MSDS
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Source E.coli
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Code CSB-EP871597HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871597HU
MSDS
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Source Baculovirus
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Code CSB-MP871597HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPATA16
Uniprot No.
Alternative Names
NYD SP12; NYD-SP12; NYDSP12; SPATA16; Spermatogenesis associated 16; Spermatogenesis-associated protein 16; SPGF6; SPT16_HUMAN; Testis development protein; Testis development protein NYD-SP12; Testis specific Golgi protein
Species
Homo sapiens (Human)
Expression Region
1-569
Target Protein Sequence
MDAGSSRSLE NAVNRIYHDQ LVPKINTSKK MSTLAHPPNI LEMSQEIKKN CGGKQVEITL ERTKMTKGIK EKQSNDLEKA AFKRKAEGEE KPTRKKQAKI TELDNQLITM PLPHIPLKNI MDVEMKLVYI DEMGVRYEFV ESFMSTGSQP TCQAAEIVDP LSVHNFSFLP QIDKWLQVAL KDASSCYRQK KYALAAGQFR TALELCSKGA VLGEPFDAPA EDIASVASFI ETKLVTCYLR MRKPDLALNH AHRSIVLNPA YFRNHLRQAT VFRCLERYSE AARSAMIADY MFWLGGGREE SISKLIKLYW QAMIEEAITR AESFSVMYTP FATKIRADKI EKVKDAFTKT HPAYAEYMYT DLQALHMLPQ TVDWSSFPPQ QYLLTLGFKN KDDGKFLEKI SSRKLPIFTE HKTPFGLTRE DTVRQMETMG KRILPILDFI RSTQLNGSFP ASSGVMEKLQ YASLLSQLQR VKEQSQVINQ AMAELATIPY LQDISQQEAE LLQSLMADAM DTLEGRRNNN ERVWNMIQKV GQIEDFLYQL EDSFLKTKKL RTARRQKTKM KRLQTVQQR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the formation of sperm acrosome, which implicated its potential role in spermatogenesis and sperm-egg fusion.
Gene References into Functions
  1. This study demonstrates that Spata16 is indispensable for male fertility in mice, as well as in humans. PMID: 29065458
  2. Mutation of SPATA16 is associated with globozoospermia. PMID: 27086357
  3. Case Report: report of a pregnancy obtained in a man with homozygous SPATA16 mutated globozoospermia. PMID: 24825417
  4. results suggest that NYD-SP12 is involved in spermatogenesis, and that NYD-SP12-encoded protein might function in the Golgi apparatus PMID: 12529416
  5. study demonstrated NYD-SP12 protein was involved in the formation of the acrosome during spermatogenesis PMID: 16372119
  6. Results indicate that NYD-SP12 evolves rapidly in both the human and the chimpanzee lineages, which is likely caused by Darwinian positive selection and/or sexual selection. PMID: 17665087
  7. consanguineous family with three affected brothers, in whom we have identified a homozygous mutation in the spermatogenesis-specific gene SPATA16. This is the first example of a nonsyndromic male infertility condition caused by an autosomal gene defect. PMID: 17847006

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Involvement in disease
Spermatogenic failure 6 (SPGF6)
Subcellular Location
Golgi apparatus. Note=Localized to the perinuclear region.
Protein Families
SPATA16 family
Tissue Specificity
Expressed in testis.
Database Links

HGNC: 29935

OMIM: 102530

KEGG: hsa:83893

STRING: 9606.ENSP00000341765

UniGene: Hs.444236

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