Recombinant Human Synemin (SYNM), partial

Code CSB-YP023019HU
MSDS
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Source Yeast
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Code CSB-EP023019HU
MSDS
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Source E.coli
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Code CSB-EP023019HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP023019HU
MSDS
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Source Baculovirus
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Code CSB-MP023019HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SYNM
Uniprot No.
Alternative Names
Desmuslin; DMN; KIAA0353; SYN; SYNEM_HUMAN; Synemin alpha ; Synemin; Synemin beta ; Synemin; intermediate filament protein ; SYNM
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteromeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteromeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells.
Gene References into Functions
  1. LMOD1, SYNPO2, PDLIM7, PLN, and SYNM down-regulation reflect the altered phenotype of smooth muscle cells in vascular disease and could be early sensitive markers of SMC dedifferentiation. PMID: 27470516
  2. alpha-Synemin localizes to the M-band of the sarcomere through interaction with the M10 region of titin PMID: 25447537
  3. Immunohistochemistry of synemin in reactive astrocytes and Rosenthal fibers in two patients with Alexander disease. There was an abundance of GFAP-positive Rosenthal fibers and widespread reactive gliosis in the white matter and subpial regions. PMID: 23594359
  4. synemin positively regulates glioblastoma cell proliferation by helping sequester PP2A away from Akt, thereby favoring Akt activation. PMID: 22337773
  5. Synemin knock-down did not influence the cytoskeleton expression and organization of human Chang liver cells PMID: 21144834
  6. SYNM could represent a novel putative breast tumor suppressor gene that is prone to epigenetic silencing. SYNM promoter methylation may become a useful predictive biomarker to stratify breast cancer patients' risk for tumor relapse. PMID: 20543860
  7. Desmuslin expression is required for the maintenance of vascular smooth muscle phenotype. Decreased desmuslin expression may affect differentiation of VSMCs and ultimately contribute to the development of varicose veins. PMID: 20573469
  8. The differences in human syncoilin and beta-synemin mRNA ratios between Duchenne muscular dystrophy and normal muscles were not statistically significant PMID: 20199207
  9. The LIM domain protein zyxin was identified as an interaction partner for human synemin. PMID: 19853601
  10. Genomic organization and single-nucleotide polymorphism map of desmuslin, a novel intermediate filament protein on chromosome 15q26.3. PMID: 11454237
  11. Expression of the intermediate filament protein synemin in myofibrillar myopathies and other muscle diseases. PMID: 12669240
  12. the default of KIAA0353 gene may play a role in the occurrence and development of varicose great saphenous vein in PDVI patients. PMID: 12887813
  13. The colocalization of Synemin [syn] with vimentin, GFAP, or neurofilament supports the idea that Syn is a key cross-linking protein in morphogenesis that connects different cytoskeletal structures PMID: 14552890
  14. Based on its localization and its expression pattern, beta-synemin functions as a structural protein involved in maintaining muscle integrity through its interactions with alpha-dystrobrevin, desmin, and other structural proteins. PMID: 15318345
  15. synemin is associated with ruffled membranes in addition to being distributed along the intermediate filament network PMID: 15657940
  16. Desmuslin is down-regulated in incompetent saphenous veins. PMID: 16476617
  17. Mammalian synemin is also shown to colocalize with dystrophin within muscle cell cultures PMID: 16777071
  18. The stability of DMN was analyzed under the action of low-level laser therapy. PMID: 17564317
  19. alpha-synemin, but not beta-synemin, interacts with both vinculin and metavinculin PMID: 18028034
  20. results revealed that synemin was modulated nearly in all human human hepatocellular carcinoma cases PMID: 18084875
  21. Results suggest human alpha-synemin plays an essential role in linking the heteropolymeric intermediate filament to adherens-type junctions, such as the costameres within mammalian striated muscle cells, via its interaction with talin. PMID: 18342854

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Subcellular Location
Cytoplasm, cytoskeleton. Cell junction, adherens junction. Note=There are at least two distinct SYNM subpopulations, one in which SYMN interacts with DES within the Z-lines, and another in which it interacts with both DTNA and DES at the costamere.
Protein Families
Intermediate filament family
Tissue Specificity
Isoform 2 is strongly detected in adult heart, fetal skeletal muscles and fetal heart. Isoform 1 is weakly detected in fetal heart and also in fetal skeletal muscle. Isoform 1 and isoform 2 are detected in adult bladder (at protein level). The mRNA is pre
Database Links

HGNC: 24466

OMIM: 606087

KEGG: hsa:23336

STRING: 9606.ENSP00000336775

UniGene: Hs.207106

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