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Recombinant Human T-cell surface antigen CD2 (CD2), partial (Active)

Recombinant Human T-cell surface antigen CD2 (CD2), partial (Active)

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Code	CSB-MP004894HU
Abbreviation	Recombinant Human CD2 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$156
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human CD2 at 5 μg/ml can bind Human CD58(CSB-MP6138HUj8-B). The EC₅₀ is 337.0-430.1 ng/mL. Biological Activity Assay The purity of CD2 was greater than 95% as determined by SEC-HPLC
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Target Background

Product Details

Purity

≥ 95% as determined by SDS-PAGE.
≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CD2 at 5 μg/mL can bind Human CD58(CSB-MP6138HUj8-B). The EC50 is 337.0-430.1 ng/mL.

Target Names

CD2

Uniprot No.

P06729

Alternative Names

T-cell surface antigen CD2; Erythrocyte receptor; LFA-2; LFA-3 receptor; Rosette receptor; T-cell surface antigen T11/Leu-5; CD2; SRBC

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

25-209aa

Target Protein Sequence

KEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD

Mol. Weight

22.7 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CD2 mediates T-cell adhesion to antigen-presenting cells and target cells through its interaction with CD58 (LFA-3), a critical checkpoint in immune synapse formation and T-cell activation. This recombinant fragment spanning residues 25–209 captures the complete extracellular immunoglobulin-like domains responsible for ligand recognition and demonstrates quantifiable binding to human CD58 with an EC50 of 337.0–430.1 ng/mL in functional ELISA, confirming that the mammalian expression system has preserved the native conformation required for receptor-ligand engagement. The validated CD2-CD58 interaction supports use in cell adhesion assays examining T-cell conjugate formation, immune cell trafficking studies modeling lymphocyte homing, and antibody development programs targeting costimulatory pathways in cancer immunotherapy. Purity exceeding 95% by both SEC-HPLC and SDS-PAGE, combined with endotoxin levels below 1.0 EU/μg, meets the quality thresholds commonly required for sensitive binding assays and functional cell-based experiments.

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Target Background

Function

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Gene References into Functions

A new prognostic model for HR-/HER2+ breast cancer based on the expression of MMP11 and CD2 was developed and the distant metastasis-free survival for patients in the high-risk group according to our model was significantly lower than that for those in the low-risk group. PMID: 28409241
These results reveal an unexpected redundancy in the human NK cell response to human cytomegalovirus and suggest that CD2 provides "signal 2" in antibody-driven adaptive NK cell responses. PMID: 27117418
Establish CD2 as a new susceptibility factor for systemic sclerosis in a European Caucasian population. PMID: 27385538
the fundamental mechanism of glycosylation of human CD2 is to promote CD2-CD58 binding by conformational adjustment of CD2 PMID: 25984915
CD58/CD2 is the primary costimulatory pathway in human CD28-CD8+ T cells. PMID: 26041540
Both the mRNA expression levels and protein expression levels of HSP27 were increased in astrocytes from POAG patients compared with those from normal control, suggesting that mutation in CD2 might pose a risk for POAG in Chinese population. PMID: 24597656
Although sMCs displayed immunoreactivity for one of the neoplastic antigens in the majority of SM patients, the aberrant CD2 and/or CD25 expression on sMCs is not as indicative of SM as the BMMC immunophenotype. PMID: 25402852
ERGdel may be a pure surrogate of CD2-positivity--which has been suggested to be a good prognositc marker in childhood ALL PMID: 24072102
Altogether, these results show that a high CD2 expression level is a hallmark of latently infected resting memory CD4(+) T cells in vivo. PMID: 23760244
Aberrant CD2 expression appears to further determine a shorter progression free survival PMID: 22634534
CD2-CD58/48 receptor-ligand interaction promotes and is required for nanotube formation in human natural killer cells. PMID: 23112830
CD2 expression does not contribute to improve the diagnosis of systemic mastocytosis when compared with aberrant CD25 expression alone. PMID: 22222639
CD2-mediated priming of resting natural killer (NK) cells is unaffected by their degree of functional maturation. PMID: 22084431
CD2 signals more strongly to S6-ribosomal protein, whereas CD28 costimulation specifically induces signaling necessary for proper NF-kappaB activation. PMID: 22013130
When isolated from multiple sclerosis patients, both nonmature and effector subsets of memory CD127(low) regulatory T cells exhibit kinetically distinct defects in suppression that are evident with CD2 pathway costimulation. PMID: 21300823
analysis of the importance of homotypic NK-to-NK cell cross-talk through 2B4/CD48 and CD2/CD58 pairs and further present their differential and overlapping roles in human NK cells PMID: 20813844
PTEN expression was up-regulated on RNA and protein level in freshly isolated human CD4(+) T cells following stimulation with CD28 or CD2. PMID: 11932928
molecules redistribute to the uropod during T cell scanning PMID: 12032326
Structural and functional studies of the extracellular domains of CD2 and CD58 and their complex. Review. PMID: 12369898
CD2BP2 is the ligand of the membrane-proximal proline-rich tandem repeat of CD2 in detergent-soluble membrane compartments, but is replaced by Fyn SH3 after CD2 is translocated into lipid rafts upon CD2 ectodomain clustering. PMID: 12426371
PSTPIP1 acts downstream of CD2/CD2AP to link CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. PMID: 12530983
This T cell surface antigen is linked to the actin-capping protein CAPZ via CMS and CIN85. PMID: 12690097
another role of the CD2-CD58 pathway that allows nonimmune and immune cells to interact directly with dendritic cells and initiate innate and adaptive immune responses. PMID: 12714509
CD2 mediates activation of the IFN-gamma intronic STAT binding region in mucosal T cells. PMID: 12731040
CD2 mediates activation of a distal -3.6-kilobase STAT5 binding region of the interferon-gamma promoter. PMID: 15528362
CD48 is a CD2 and CD244 (2B4)-binding protein PMID: 16803907
We use this analysis to determine that the 2D Kd for CD2-CD58 is 5.4-7.6 molecules/microm2. 2D Kd analysis provides a general and quantitative measure of the mechanisms regulating cell-cell adhesion. PMID: 17085486
T cell activation causes the CD58-bound CD2 to be recognized and immobilized at sites of cell-cell contact, thereby strengthening T cell-APC adhesion PMID: 17168569
results showed that the expression of CD2 significantly increased with the severity of chronic HBV infection, which suggested that CD2 might contribute to the hepatocyte damage in chronic HBV infection PMID: 18318997
Data suggest that detection of CD2 or CD13 expression in chronic lymphocytic leukemia (CLL) suggests familial CLL, and that CD38 expression does not carry the negative prognosis observed in sporadic CLL. PMID: 18431797
the synergistic synthesis of IL-8 occurs when lymphocytes are stimulated through the CD2 pathway by CD58 on HT-29 cells, resulting in TNF-alpha release that, in turn, augments IL-8 synthesis and CD58 expression by the HT-29 cells PMID: 19109405
during clinical remission, increases in CD58 expression, mediated by the protective allele, up-regulate the expression of FoxP3 through engagement of the CD58 receptor, CD2, leading to the enhanced function of TREG cells that are defective in MS PMID: 19237575
CD2 functions as the master switch recruiting CD48 and Lck PMID: 19494291
LFA-1 and CD2 synergize for the Erk1/2 activation in the Natural Killer (NK) cell immunological synapse PMID: 19502238
CD244 inhibition and activation depends on CD2 and phospholipase C-gamma1 PMID: 19586919
Genetic variants at CD2 are associated with rheumatoid arthritis risk PMID: 19898481
CD2-CD58 binding site PMID: 11575926