Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

DTYMK

Uniprot No.

P23919

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

CDC8; Deoxythymidylate kinase (thymidylate kinase); deoxythymidylate kinase; dTMP kinase; Dtymk; FLJ44192; KTHY_HUMAN; MGC198617; OTTHUMP00000164604; OTTHUMP00000200145; OTTHUMP00000200146; PP3731; Thymidylate kinase; TMPK; TYMK

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-212aa

Target Protein Sequence

MAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGELWK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

50.7kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human DTYMK covers amino acids 1-212. The calculated molecular weight for this DTYMK protein is 50.8 kDa. Expression of this DTYMK protein is conducted in e.coli. The DTYMK gene fragment has been modified by fusing the N-terminal GST tag, providing convenience in detecting and purifying the recombinant DTYMK protein during the following stages.

Thymidylate kinase (DTYMK) is a crucial enzyme involved in nucleotide metabolism, specifically in the synthesis of DNA. It catalyzes the phosphorylation of dTMP (deoxythymidine monophosphate) to form dTDP (deoxythymidine diphosphate). This reaction is essential for the deoxythymidine triphosphate (dTTP) biosynthesis pathway, a precursor necessary for DNA replication and repair. DTYMK contributes to the maintenance of proper nucleotide pools within cells, ensuring the availability of building blocks for DNA synthesis. Its activity is tightly regulated to support genomic integrity and cellular proliferation. Understanding the function of DTYMK provides insights into the broader cellular processes involved in DNA replication and repair, offering potential targets for therapeutic interventions in conditions where nucleotide metabolism is dysregulated.

Target Background

Function

Catalyzes the conversion of dTMP to dTDP.

Gene References into Functions

Lentiviral delivery of small hairpin RNA targeting TMPK in combination with a low dose of doxorubicin as a new approach to kill colon cancer cells regardless of p53 status. PMID: 18413751

Protein Families

Thymidylate kinase family

Database Links

HGNC: 3061

OMIM: 188345

KEGG: hsa:1841

STRING: 9606.ENSP00000304802

UniGene: Hs.471873

Code	CSB-EP007226HU
Abbreviation	Recombinant Human DTYMK protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Thymidylate kinase (DTYMK)

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