Recombinant Human Thymidylate kinase (DTYMK)

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Code CSB-EP007226HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
DTYMK
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
CDC8; Deoxythymidylate kinase (thymidylate kinase); deoxythymidylate kinase; dTMP kinase; Dtymk; FLJ44192; KTHY_HUMAN; MGC198617; OTTHUMP00000164604; OTTHUMP00000200145; OTTHUMP00000200146; PP3731; Thymidylate kinase; TMPK; TYMK
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-212aa
Target Protein Sequence
MAARRGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVEDHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGLPKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWKMVDASKSIEAVHEDIRVLSEDAIRTATEKPLGELWK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
50.7kDa
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The expression region of this recombinant Human DTYMK covers amino acids 1-212. The calculated molecular weight for this DTYMK protein is 50.8 kDa. Expression of this DTYMK protein is conducted in e.coli. The DTYMK gene fragment has been modified by fusing the N-terminal GST tag, providing convenience in detecting and purifying the recombinant DTYMK protein during the following stages.

Thymidylate kinase (DTYMK) is a crucial enzyme involved in nucleotide metabolism, specifically in the synthesis of DNA. It catalyzes the phosphorylation of dTMP (deoxythymidine monophosphate) to form dTDP (deoxythymidine diphosphate). This reaction is essential for the deoxythymidine triphosphate (dTTP) biosynthesis pathway, a precursor necessary for DNA replication and repair. DTYMK contributes to the maintenance of proper nucleotide pools within cells, ensuring the availability of building blocks for DNA synthesis. Its activity is tightly regulated to support genomic integrity and cellular proliferation. Understanding the function of DTYMK provides insights into the broader cellular processes involved in DNA replication and repair, offering potential targets for therapeutic interventions in conditions where nucleotide metabolism is dysregulated.

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Target Background

Function
Catalyzes the conversion of dTMP to dTDP.
Gene References into Functions
  1. Lentiviral delivery of small hairpin RNA targeting TMPK in combination with a low dose of doxorubicin as a new approach to kill colon cancer cells regardless of p53 status. PMID: 18413751
Protein Families
Thymidylate kinase family
Database Links

HGNC: 3061

OMIM: 188345

KEGG: hsa:1841

STRING: 9606.ENSP00000304802

UniGene: Hs.471873

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