Name: Recombinant Human Transcobalamin-1 (TCN1)
Brand: CUSABIO
SKU: CSB-YP023317HU

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

TCN1

Uniprot No.

P20061

Research Area

Signal Transduction

Alternative Names

Haptocorin; Haptocorrin; HC; OTTHUMP00000235879; TC 1; TC I; TC-1; TC1; TCI; TCN 1; TCN1; TCO1_HUMAN; Transcobalamin 1; Transcobalamin I; Transcobalamin-1; Transcobalamin1; TranscobalaminI; Vitamin B12 binding protein; Vitamin B12 binding protein R binder family

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

24-433aa

Target Protein Sequence

EICEVSEENYIRLKPLLNTMIQSNYNRGTSAVNVVLSLKLVGIQIQTLMQKMIQQIKYNVKSRLSDVSSGELALIILALGVCRNAEENLIYDYHLIDKLENKFQAEIENMEAHNGTPLTNYYQLSLDVLALCLFNGNYSTAEVVNHFTPENKNYYFGSQFSVDTGAMAVLALTCVKKSLINGQIKADEGSLKNISIYTKSLVEKILSEKKENGLIGNTFSTGEAMQALFVSSDYYNENDWNCQQTLNTVLTEISQGAFSNPNAAAQVLPALMGKTFLDINKDSSCVSASGNFNISADEPITVTPPDSQSYISVNYSVRINETYFTNVTVLNGSVFLSVMEKAQKMNDTIFGFTMEERSWGPYITCIQGLCANNNDRTYWELLSGGEPLSQGAGSYVVRNGENLEVRWSKY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

47.6kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The product CSB-YP023317HU is a recombinant human transcobalamin-1 (TCN1) expressed in the yeast. Its target gene sequence corresponds to amino acids 24-433 of the human TCN1. The 6xHis-tag is fused to the protein at the N-terminus. Its purity is greater than 90% determined by SDS-PAGE. It migrated to the molecular weight band of 45-50 kDa. This recombinant TCN1 protein is a full-length of the mature protein. It is available now. The target protein TCN1 is a glycoprotein produced by the salivary glands of the mouth. The primary function of this protein is to protect cobalamin (Vitamin B12) from acid degradation in the stomach by producing a Haptocorrin-Vitamin B12 complex.

Target Background

Function

Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach.

Gene References into Functions

Higher placental TC protein abundance was associated with higher cord blood vitamin B12 concentrations, suggesting a potential role in vitamin B12 transport to the fetus. PMID: 27577703
The variant rs526934 from the TCN1 gene was associated with an increased risk of developing gastric cancer. PMID: 26959381
Levels of holotranscobalamin are decreased patients with cobalamin deficiency. PMID: 24057896
Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin. PMID: 23846701
TCN1 gene expression implicates disease progression in patient with middle ear cholesteatoma. PMID: 23670528
comparison of human and rainbow trout cobalamin-binding protein PMID: 22872637
Elevated concentrations of B(12) found in autoimmune lymphoproliferative syndrome patients were due to increased lymphocyte expression of haptocorrin. PMID: 22306884
Stromal expression of KRT15, TCN1, and HOXB13 was significantly correlated with tumor grade, stromal hypercellularity, mitotic activity and microscopic borders. PMID: 21574054
two novel mutations, each causing a premature stop codon - a genetic basis for TC I deficiency PMID: 19686235
Comparative analysis of cobalamin binding kinetics PMID: 11788601
No association between the MTHFR A1298C and transcobalamin C776G genetic polymorphisms and hyperhomocysteinemia in thrombotic disease. PMID: 12590948
interactions of COBALAMIN BINDING proteins with a number of ligands PMID: 17487979
Maternal folate-related polymorphisms studied here (CBS, MTR, RFC-1, and TC) have no influence on trisomy 21 susceptibility in subjects of Brazilian population.v PMID: 19729796

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Subcellular Location

Secreted.

Protein Families

Eukaryotic cobalamin transport proteins family

Tissue Specificity

Produced by the salivary glands of the oral cavity, in response to ingestion of food. Major constituent of secondary granules in neutrophils.

Database Links

HGNC: 11652

OMIM: 189905

KEGG: hsa:6947

STRING: 9606.ENSP00000257264

UniGene: Hs.2012

Code	CSB-YP023317HU
Abbreviation	Recombinant Human TCN1 protein
MSDS	MSDS Datasheet
Size	$250
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Transcobalamin-1 (TCN1)

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