Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

TRIM72

Uniprot No.

Q6ZMU5

Research Area

Transport

Alternative Names

Mitsugumin-53 Short name: Mg53

Species

Homo sapiens (Human)

Source

Baculovirus

Expression Region

1-269aa

Target Protein Sequence

MSAAPGLLHQELSCPLCLQLFDAPVTAECGHSFCRACLGRVAGEPAADGTVLCPCCQAPTRPQALSTNLQLARLVEGLAQVPQGHCEEHLDPLSIYCEQDRALVCGVCASLGSHRGHRLLPAAEAHARLKTQLPQQKLQLQEACMRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRVFLAALEGSLDREAERVRGEAGVALRRELGSLNSYLEQLRQMEKVLEEVADKPQTEFLMKYCLVTSRLQKILAESPPPARLDIQLPIIS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

33.4 kDa

Protein Length

partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human TRIM72 contains amino acids 1-269. The calculated molecular weight for this TRIM72 protein is 33.4 kDa. This TRIM72 protein is produced using baculovirus expression system. The TRIM72 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant TRIM72 protein during the following stages.

The human tripartite motif-containing protein 72 (TRIM72), also known as MG53, is a member of the tripartite motif (TRIM) protein family. TRIM72 is predominantly expressed in skeletal and cardiac muscle tissues. It plays a crucial role in membrane repair and maintenance of muscle integrity. TRIM72 is involved in the repair of plasma membrane injuries in response to mechanical stress or damage. It facilitates the resealing of membrane disruptions by interacting with phosphatidylserine exposed to the injured membrane. Additionally, TRIM72 has been implicated in various physiological processes, including muscle regeneration and adaptation to exercise. Understanding TRIM72's functions may have implications for therapeutic strategies targeting muscle-related disorders and injuries.

Target Background

Function

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.

Gene References into Functions

serum TRIM72 may be a potential biomarker for the diagnosis and the prognosis of colon cancer. PMID: 29806630
MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
Zn(2+) interacts with MG53 in protection against injury to the cell membrane PMID: 25869134
MG53 induces FAK ubiquitination with the aid of UBE2H during skeletal myogenesis. PMID: 24344130
MG53/TRIM72 protein can be directly applied as a therapeutic agent to increase membrane repair capacity of many cell types. PMID: 23699904
data reveal NM-IIA as a key cytoskeleton motor protein that facilitates vesicle trafficking during MG53-mediated cell membrane repair PMID: 22253476
membrane-delimited interaction between MG53 and PTRF contributes to initiation of cell membrane repair PMID: 21343302
MG53, annexin A1, and dysferlin localize to the t-tubule network and show enriched labeling at longitudinal tubules of the t-system in overstretch PMID: 21412170
Crystal structure of PRY-SPRY domain of human TRIM72. PMID: 19967786
Observational study and meta-analysis of gene-disease association. (HuGE Navigator) PMID: 19129174

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Subcellular Location

Cell membrane, sarcolemma. Cytoplasmic vesicle membrane.

Protein Families

TRIM/RBCC family

Database Links

HGNC: 32671

OMIM: 613288

KEGG: hsa:493829

STRING: 9606.ENSP00000312675

UniGene: Hs.661859

Code	CSB-BP744394HU
Abbreviation	Recombinant Human TRIM72 protein, partial
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Tripartite motif-containing protein 72 (TRIM72), partial

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