Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
Mitsugumin-53
Short name:
Mg53
Species
Homo sapiens (Human)
Expression Region
1-269aa
Target Protein Sequence
MSAAPGLLHQELSCPLCLQLFDAPVTAECGHSFCRACLGRVAGEPAADGTVLCPCCQAPTRPQALSTNLQLARLVEGLAQVPQGHCEEHLDPLSIYCEQDRALVCGVCASLGSHRGHRLLPAAEAHARLKTQLPQQKLQLQEACMRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRVFLAALEGSLDREAERVRGEAGVALRRELGSLNSYLEQLRQMEKVLEEVADKPQTEFLMKYCLVTSRLQKILAESPPPARLDIQLPIIS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Human TRIM72 contains amino acids 1-269. The calculated molecular weight for this TRIM72 protein is 33.4 kDa. This TRIM72 protein is produced using baculovirus expression system. The TRIM72 gene fragment has been modified by fusing the N-terminal 10xHis tag and C-terminal Myc tag, providing convenience in detecting and purifying the recombinant TRIM72 protein during the following stages.
The human tripartite motif-containing protein 72 (TRIM72), also known as MG53, is a member of the tripartite motif (TRIM) protein family. TRIM72 is predominantly expressed in skeletal and cardiac muscle tissues. It plays a crucial role in membrane repair and maintenance of muscle integrity. TRIM72 is involved in the repair of plasma membrane injuries in response to mechanical stress or damage. It facilitates the resealing of membrane disruptions by interacting with phosphatidylserine exposed to the injured membrane. Additionally, TRIM72 has been implicated in various physiological processes, including muscle regeneration and adaptation to exercise. Understanding TRIM72's functions may have implications for therapeutic strategies targeting muscle-related disorders and injuries.
