Home
Protein
Recombinant Proteins
Recombinant Human Tripartite motif-containing protein 72 (TRIM72), partial

Recombinant Human Tripartite motif-containing protein 72 (TRIM72), partial

^{In Stock}

Code	CSB-EP744394HU1
Abbreviation	Recombinant Human TRIM72 protein, partial
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

TRIM72

Uniprot No.

Q6ZMU5

Research Area

Cell Biology

Alternative Names

LOC493829; Mg53; Mitsugumin-53; TRI72_HUMAN; trim72; Tripartite motif containing 72; Tripartite motif containing protein 72 ; Tripartite motif-containing protein 72

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-477aa

Target Protein Sequence

SAAPGLLHQELSCPLCLQLFDAPVTAECGHSFCRACLGRVAGEPAADGTVLCPCCQAPTRPQALSTNLQLARLVEGLAQVPQGHCEEHLDPLSIYCEQDRALVCGVCASLGSHRGHRLLPAAEAHARLKTQLPQQKLQLQEACMRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRVFLAALEGSLDREAERVRGEAGVALRRELGSLNSYLEQLRQMEKVLEEVADKPQTEFLMKYCLVTSRLQKILAESPPPARLDIQLPIISDDFKFQVWRKMFRALMPALEELTFDPSSAHPSLVVSSSGRRVECSEQKAPPAGEDPRQFDKAVAVVAHQQLSEGEHYWEVDVGDKPRWALGVIAAEAPRRGRLHAVPSQGLWLLGLREGKILEAHVEAKEPRALRSPERRPTRIGLYLSFGDGVLSFYDASDADALVPLFAFHERLPRPVYPFFDVCWHDKGKNAQPLLLVGPEGAEA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

79.4 kDa

Protein Length

Partial

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The synthesis of this Recombinant Human TRIM72 protein depends on the utilization of recombinant DNA technology to a large degree. DNA sequences that encoded the TRIM72 protein could be inserted into a vector and introduced into an expression host, E.coli, where it could be easily expressed in and purified from. The expression of this TRIM72 protein was at 2-477aa. N-terminal GST tag was fused with this protein. The purity is 85%+ determined by SDS-PAGE.

TRIM72 (also known as MG53) is gene encoding a protein named tripartite motif-containing protein 72 (TRIM72) or mitsugumin-53 (Mg53) in human. The protein encoded by this gene is a muscle-specific TRIM family protein. This protein is initially identified as a key regulator of cell membrane repair machinery. It has been suggested to be a critical regulator of muscle insulin signaling pathway by acting as ubiquitin E3 ligase targeting both the insulin receptor and insulin receptor substrate 1 (IRS1).

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.

Gene References into Functions

serum TRIM72 may be a potential biomarker for the diagnosis and the prognosis of colon cancer. PMID: 29806630
MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
Zn(2+) interacts with MG53 in protection against injury to the cell membrane PMID: 25869134
MG53 induces FAK ubiquitination with the aid of UBE2H during skeletal myogenesis. PMID: 24344130
MG53/TRIM72 protein can be directly applied as a therapeutic agent to increase membrane repair capacity of many cell types. PMID: 23699904
data reveal NM-IIA as a key cytoskeleton motor protein that facilitates vesicle trafficking during MG53-mediated cell membrane repair PMID: 22253476
membrane-delimited interaction between MG53 and PTRF contributes to initiation of cell membrane repair PMID: 21343302
MG53, annexin A1, and dysferlin localize to the t-tubule network and show enriched labeling at longitudinal tubules of the t-system in overstretch PMID: 21412170
Crystal structure of PRY-SPRY domain of human TRIM72. PMID: 19967786
Observational study and meta-analysis of gene-disease association. (HuGE Navigator) PMID: 19129174