Human Tripartite motif-containing protein 72(TRIM72) ELISA kit

Code CSB-EL024511HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
tripartite motif-containing 72
Alternative Names
LOC493829 ELISA Kit; Mg53 ELISA Kit; Mitsugumin-53 ELISA Kit; TRI72_HUMAN ELISA Kit; trim72 ELISA Kit; Tripartite motif containing 72 ELISA Kit; Tripartite motif containing protein 72 ELISA Kit; Tripartite motif-containing protein 72 ELISA Kit
Abbreviation
TRIM72
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TRIM72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %89-98
1:2Average %92
Range %86-95
1:4Average %85
Range %80-89
1:8Average %92
Range %86-97
Recovery
The recovery of human TRIM72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10398-107
EDTA plasma (n=4)9590-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.508 2.310 2.409 2.284
8001.442 1.345 1.394 1.269
4000.779 0.816 0.798 0.673
2000.463 0.446 0.455 0.330
1000.322 0.317 0.320 0.195
500.239 0.231 0.235 0.110
250.198 0.192 0.195 0.070
00.126 0.124 0.125
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human TRIM72 ELISA Kit was designed for the quantitative measurement of Human TRIM72 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
Gene References into Functions
  1. serum TRIM72 may be a potential biomarker for the diagnosis and the prognosis of colon cancer. PMID: 29806630
  2. MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
  3. MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
  4. Zn(2+) interacts with MG53 in protection against injury to the cell membrane PMID: 25869134
  5. MG53 induces FAK ubiquitination with the aid of UBE2H during skeletal myogenesis. PMID: 24344130
  6. MG53/TRIM72 protein can be directly applied as a therapeutic agent to increase membrane repair capacity of many cell types. PMID: 23699904
  7. data reveal NM-IIA as a key cytoskeleton motor protein that facilitates vesicle trafficking during MG53-mediated cell membrane repair PMID: 22253476
  8. membrane-delimited interaction between MG53 and PTRF contributes to initiation of cell membrane repair PMID: 21343302
  9. MG53, annexin A1, and dysferlin localize to the t-tubule network and show enriched labeling at longitudinal tubules of the t-system in overstretch PMID: 21412170
  10. Crystal structure of PRY-SPRY domain of human TRIM72. PMID: 19967786
  11. Observational study and meta-analysis of gene-disease association. (HuGE Navigator) PMID: 19129174

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Subcellular Location
Cell membrane, sarcolemma. Cytoplasmic vesicle membrane.
Protein Families
TRIM/RBCC family
Database Links

HGNC: 32671

OMIM: 613288

KEGG: hsa:493829

STRING: 9606.ENSP00000312675

UniGene: Hs.661859

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