Recombinant Human Tubulin beta-8 chain (TUBB8)

Code CSB-YP676987HU
MSDS
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Source Yeast
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Code CSB-EP676987HU
MSDS
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Source E.coli
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Code CSB-EP676987HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP676987HU
MSDS
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Source Baculovirus
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Code CSB-MP676987HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TUBB8
Uniprot No.
Alternative Names
bA631M21.2; Class VIII beta tubulin; HSA10p15 beta tubulin 4Q; RP11-631M21.2; TBB8_HUMAN; TUBB8; Tubulin beta 8; Tubulin beta 8 chain; Tubulin beta 8 class VIII; Tubulin beta-8 chain
Species
Homo sapiens (Human)
Expression Region
1-444
Target Protein Sequence
MREIVLTQIG QCGNQIGAKF WEVISDEHAI DSAGTYHGDS HLQLERINVY YNEASGGRYV PRAVLVDLEP GTMDSVRSGP FGQVFRPDNF IFGQCGAGNN WAKGHYTEGA ELMESVMDVV RKEAESCDCL QGFQLTHSLG GGTGSGMGTL LLSKIREEYP DRIINTFSIL PSPKVSDTVV EPYNATLSVH QLIENADETF CIDNEALYDI CSKTLKLPTP TYGDLNHLVS ATMSGVTTCL RFPGQLNADL RKLAVNMVPF PRLHFFMPGF APLTSRGSQQ YRALTVAELT QQMFDAKNMM AACDPRHGRY LTAAAIFRGR MPMREVDEQM FNIQDKNSSY FADWLPNNVK TAVCDIPPRG LKMSATFIGN NTAIQELFKR VSEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVS EYQQYQDATA EEEEDEEYAE EEVA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB8 has a key role in meiotic spindle assembly and oocyte maturation.
Gene References into Functions
  1. This TUBB8 variant in one infertile individual was absent in the 100 fertile females and was predicted to be highly damaging to the function of the TUBB8 protein by SIFT and PolyPhen-2. PMID: 29671363
  2. A homozygous mutation of TUBB8 associated with complete cleavage failure in fertilized eggs. PMID: 29704226
  3. TUBB8 mutations occur in a high proportion of infertile women with oocyte maturation arrest. PMID: 28652098
  4. The results not only emphasize the important role of TUBB8 in oocyte maturation, fertilization and early embryonic development but they also provide a basis for determining the genetic variations in TUBB8 as a potential additional criterion for evaluating the quality of patients' functional PB1 oocytes. PMID: 27989988
  5. 9 novel TUBB8 mutations and phenotypes in patients with oocyte maturation arrest were reported. PMID: 27273344
  6. TUBB8 mutations have dominant-negative effects that disrupt microtubule behavior and oocyte meiotic spindle assembly and maturation, causing female infertility. PMID: 26789871

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Involvement in disease
Oocyte maturation defect 2 (OOMD2)
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle.
Protein Families
Tubulin family
Tissue Specificity
Expressed at a high level in oocytes, at different stages of development.
Database Links

HGNC: 20773

OMIM: 616768

KEGG: hsa:347688

STRING: 9606.ENSP00000328808

UniGene: Hs.532659

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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