Recombinant Human Ubiquitin carboxyl-terminal hydrolase 47 (USP47), partial

Code CSB-YP856964HU
MSDS
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Source Yeast
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Code CSB-EP856964HU
MSDS
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Source E.coli
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Code CSB-EP856964HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856964HU
MSDS
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Source Baculovirus
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Code CSB-MP856964HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
USP47
Uniprot No.
Alternative Names
Deubiquitinating enzyme 47; TFRP; Trf TATA binding protein related factor proximal homolog; Trf TATA binding protein related factor proximal homolog Drosophila; Ubiquitin carboxyl-terminal hydrolase 47; Ubiquitin specific peptidase 47; Ubiquitin specific processing protease 47; Ubiquitin specific protease 47; Ubiquitin thioesterase 47; Ubiquitin thiolesterase 47; Ubiquitin-specific-processing protease 47; UBP47_HUMAN; USP47
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.
Gene References into Functions
  1. these results suggest that USP47 may play distinct roles in ubiquitin-mediated cellular processes via de-ubiquitinating enzymes activity. PMID: 26115687
  2. miR-204-5p acts as a tumor suppressor in gastric cancer through inhibiting USP47 and RAB22A. PMID: 25429829
  3. USP47 is a deubiquitinase that prevents beta-catenin ubiquitination. PMID: 26169834
  4. Data indicate that E-cadherin ubiquitination consistently increases after depletion of kinesin-like protein KIFC3 or ubiquitin-specific protease USP47. PMID: 25253721
  5. The results of this study indicated that USP47 plays a crucial role in the control of axonal growth during neuronal development by antagonizing CHIP-mediated katanin-p60 degradation. PMID: 23904609
  6. USP47 has a role in regulating DNA repair and maintaining genome integrity PMID: 21362556
  7. USP47, a novel beta-Trcp interactor, regulates cell growth and survival, potentially providing a novel target for anticancer therapies. PMID: 19966869

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Subcellular Location
Cytoplasm.
Protein Families
Peptidase C19 family
Tissue Specificity
Expressed in skeletal muscle, heart and testis.
Database Links

HGNC: 20076

OMIM: 614460

KEGG: hsa:55031

STRING: 9606.ENSP00000339957

UniGene: Hs.577256

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