Recombinant Human Ubiquitin carboxyl-terminal hydrolase isozyme L1(UCHL1),partial

Code CSB-RP116374h
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names UCHL1
Uniprot No. P09936
Research Area Neuroscience
Alternative Names Epididymis luminal protein 117; Epididymis secretory protein Li 53; HEL 117; HEL S 53; NDGOA; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; Park 5; PARK5; PGP 9.5; PGP9.5; PGP95; Protein gene product 9.5; Ubiquitin C terminal esterase L1; Ubiquitin C terminal hydrolase; Ubiquitin C terminal hydrolase L1; Ubiquitin carboxyl terminal esterase L1; Ubiquitin carboxyl terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; Ubiquitin thiolesterase; Ubiquitin thiolesterase L1; UCH-L1; UCHL1; UCHL1_HUMAN
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-222aa
Target Protein Sequence MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 28.8kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Gene References into Functions
  1. The proteins UCH-L1 and alpha-internexin could be independent prognostic biomarkers of pancreatic neuroendocrine tumors. PMID: 28526880
  2. We have also demonstrated that UCHL1 S-nitrosylation provides seeding for faster aggregation of a-synuclein. Finally, the in vitro nitrosylation of UCHL1 was corroborated with rotenone induced mouse model of PD PMID: 28300150
  3. In newborns undergoing cardiac surgery, ubiquitin C-terminal hydrolase 1 showed a significant rise at 0 hours in the deep hypothermic circulatory arrest group compared to baseline. Levels returned to baseline at 12 hours. There was an early rise in UCHL1 at 0 hours in the cardiopulmonary bypass group. PMID: 29945509
  4. Overexpression of UCHL1 genes promoted apoptosis in cells treated with VER+ADM. UCHL1 knockdown using siRNA weakened the effect of ADM+VER, indicating that ADM+VER promotes HCC cell apoptosis and that UCHL1 genes participate in VER-mediated promotion in tumor cell apoptosis. PMID: 29627846
  5. Cholangiocarcinoma patients who had high methylation of HTATIP2 and low methylation of UCHL1 showed longer overall survival than those with low HTATIP2 methylation and high UCHL1 methylation. PMID: 29359783
  6. High UCHL1 expression is associated with posterior longitudinal ligament ossification. PMID: 29782494
  7. Prx II exhibited more effective molecular chaperone activity than Prx I when UCH-L1 was the client. Prx II interacted with UCH-L1 through its C-terminal region to protect UCH-L1 from thermal or oxidative inactivation PMID: 29339092
  8. Data report that the expression of UCH-L1 is significantly higher in breast cancer cells with higher invasive ability. Also, the overexpression of UCH-L1 led to activation of Akt and phosphorylation. Thus, these findings demonstrated that UCH-L1 promotes invasion of breast cancer cells. PMID: 28636190
  9. The authors observed higher serum levels of GFAP and UCH-L1 in brain-injured children compared with controls and also demonstrated a step-wise increase of biomarker concentrations over the continuum of severity from mild to severe traumatic brain injury. Serum UCH-L1 and GFAP concentrations also strongly predicted poor outcome. PMID: 27319802
  10. UCHL1 inhibits autophagy and is depending on its de-ubiquitinating activity. PMID: 29462615
  11. Uchl1 concentrations in the blood plasma of boys with cryptorchidism, may reflect the heat-induced apoptosis of germ cells PMID: 29401475
  12. Overexpression of UCHL1 is associated with thermal injury. PMID: 28193576
  13. UCHL1 localizes to TTAGGG repeats at telomeres and interstitial telomeric sequences. A weak or transient interaction between UCHL1 and the shelterin complex was confirmed by immunoprecipitation and proximity ligation assays. PMID: 29126443
  14. novel biomarker for identifying malignant potential of primary well-differentiated and moderately differentiated pancreatic neuroendocrine tumors PMID: 29150024
  15. detection of significantly higher levels of UCH-L1 in patients with ischemic stroke and intracranial hemorrhage compared to patients with metabolic disorder induced impaired consciousness and healthy volunteers PMID: 28651886
  16. This study also showed that UCH-L1 promotes angiogenesis of HUVECs, as well as invasion in cancer cells, by up-regulating ROS by deubiquitination of NOX4, suggesting that UCH-L1 plays a key role in angiogenesis of HUVECS by regulating ROS levels by deubiquitination of NOX4. PMID: 29128359
  17. Familial mutations and post-translational modifications of UCH-L1 in Parkinson's disease have been summarized. (Review) PMID: 26899237
  18. Aberrant expression of UCHL1 in pediatric high-grade gliomas may promote cell invasion, transformation, and self-renewal properties, at least in part, by modulating Wnt/Beta catenin activity PMID: 28472177
  19. Suppression of MITF activity by UCHL1 via protein degradation might aid in the development of new therapeutic approaches for melanoma or dyspigmentation disorders. PMID: 28392346
  20. Overexpression of UCH-L1 in MCF7 cells up-regulated MDR1, CD147, MMP2, and MMP9, which conferred multidrug resistance and promoted migration/invasion. PMID: 26293643
  21. Folding of a 52-Knotted Protein PMID: 28002735
  22. This study demonstrated that UCH-L1 was detectible within 1 hour of injury and rose rapidly and peaked at 8 hours after injury and declined rapidly over 48 hours. PMID: 27018834
  23. This study establishes the importance of UCHL1 in neurodegeneration, provides new mechanistic insight about ubiquitin processing, and underlines the complexity of the different roles of UCHL1 PMID: 28007905
  24. TGF-beta1 can promote PGP9.5 expression in Cancer associated fibroblasts to facilitate the growth of cancer cells. PMID: 27840994
  25. PGP9.5 is also expressed in benign fibroblastic lesions PMID: 27914685
  26. cytoplasmic accumulation of P53 was strongly associated with the unmethylated UCHL1 profile (P = 0.006), supporting the relationship between these two proteins in sporadic colorectal cancer PMID: 26314856
  27. indicated that the LDT is an accurate, robust and automated assay, which adequately and reliably identifies patients presenting with small fiber neuropathy, and therefore has potential for use in large scale clinical studies PMID: 27215701
  28. No difference in cord blood concentration found between hypoxic-ischemic encephalopathy neonates and controls PMID: 26135781
  29. UCHL1 has a role in Alzheimer Disease [review] PMID: 26881020
  30. UCHL1 expression is highly upregulated upon hepatic stellate cell (HSC) activation and is involved in the regulation of HSC proliferation PMID: 26264933
  31. UCH-L1 is a promising prognostic biomarker for GCAs and might play an important role in the carcinogenesis of gastric cancer. PMID: 26823707
  32. Folloing aneurysmal subarachnoid hemorrhage, eleevated UCH-L1 levels during the five-day follow-up were associated with unfavorable neurological outcome. PMID: 26810533
  33. UCH-L1 plays a crucial role in modulating the degradation of EGFR and promoting malignant properties in multi-drug resistant breast cancer PMID: 26722437
  34. High UCHL1 expression is associated with multiple myeloma. PMID: 26513019
  35. The neuronal marker UCH-L1 is induced in, and specifically augments the oncogene-induced transformation of, germinal center B cells. PMID: 26702068
  36. The promoter methylation degree of FLNC, THBS1, UCHL1, and DLEC1 in serum could tell the existence of GC and only UCHL1 in the serum was also associated with poor prognosis of GC. PMID: 26550574
  37. The results of this study suggest the role for UCHL1 in promoting Corticospinal motor neurons health and stability. PMID: 25596590
  38. UCH-L1 is significantly associated with outcome, but it does not add predictive power to commonly used prognostic variables in a population of patients with TBI of varying severities. PMID: 26547005
  39. Serum UCH-l1 levels serve as a novel biomarker for neuronal damage from white matter lesions. PMID: 26232084
  40. High Ubiquitin C-terminal hydrolase-L1 increases cancer cell invasion by modulating hydrogen peroxide generated via NADPH oxidase 4. PMID: 25915537
  41. These findings demonstrated that UCHL1 promoted cell proliferation, migration, and invasion depending on its de-ubiquitinase activity by activating Akt and Erk1/2 pathways PMID: 26018507
  42. These results suggest that ubiquitin C-terminal hydrolase and alphaII-spectrin breakdown product 145 kDa may be useful in assessing outcome after pediatric traumatic brain injury. PMID: 22022780
  43. Pathogenetic tau truncation may contribute to synaptic deterioration in Alzheimer disease by aberrant recruitment of Parkin and UCHL-1 to mitochondria. PMID: 25687137
  44. UCHL1 promotes metastases as a deubiquitinating enzyme for HIF-1alpha. PMID: 25615526
  45. Meta-analysis suggesting that UCHL1 S18Y polymorphism is moderately associated with susceptibility to Parkinson's disease PMID: 25370916
  46. Potential of UCHL1 as biomarker for destruction of pancreatic beta cells. PMID: 25638021
  47. Data identified a variant of UCH-L1 lacking N-terminal 11 amino acids designated as NT-UCH-L1 and was found to have a protective role in the Parkinson's disease model in vitro and in vivo. PMID: 24959670
  48. UCHL1 may delay Alzheimer's progression by regulating APP degradation. PMID: 25466238
  49. miR-922 increasing the levels of phosphorylated tau by regulating UCHL1 levels contributed to the pathogenesis of Alzheimer diseases. PMID: 24950120
  50. Skin PGP 9.5 expression, a neuronal marker, was significantly lower in patients with familial transthyretin amyloid neuropathy compared to controls. PMID: 25973863

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Involvement in disease Parkinson disease 5 (PARK5); Spastic paraplegia 79, autosomal recessive (SPG79)
Subcellular Location Cytoplasm, Endoplasmic reticulum membrane, Lipid-anchor
Protein Families Peptidase C12 family
Tissue Specificity Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and A
Database Links

HGNC: 12513

OMIM: 191342

KEGG: hsa:7345

STRING: 9606.ENSP00000284440

UniGene: Hs.518731

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