Recombinant Human Ubiquitin-conjugating enzyme E2 Z (UBE2Z)

Code CSB-YP875692HU
MSDS
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Source Yeast
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Code CSB-EP875692HU
MSDS
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Source E.coli
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Code CSB-EP875692HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875692HU
MSDS
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Source Baculovirus
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Code CSB-MP875692HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UBE2Z
Uniprot No.
Alternative Names
FLJ13855; GC17P044341; HOYS7; Uba6 specific E2 conjugating enzyme 1; UBA6 specific enzyme E2; Uba6-specific E2 conjugating enzyme 1; UBE2Z; UBE2Z_HUMAN; Ubiquitin carrier protein Z; Ubiquitin conjugating enzyme E2 Z; Ubiquitin conjugating enzyme E2Z; Ubiquitin protein ligase Z; Ubiquitin-conjugating enzyme E2 Z; Ubiquitin-protein ligase Z; Use1
Species
Homo sapiens (Human)
Expression Region
1-354
Target Protein Sequence
MAESPTEEAA TAGAGAAGPG ASSVAGVVGV SGSGGGFGPP FLPDVWAAAA AAGGAGGPGS GLAPLPGLPP SAAAHGAALL SHWDPTLSSD WDGERTAPQC LLRIKRDIMS IYKEPPPGMF VVPDTVDMTK IHALITGPFD TPYEGGFFLF VFRCPPDYPI HPPRVKLMTT GNNTVRFNPN FYRNGKVCLS ILGTWTGPAW SPAQSISSVL ISIQSLMTEN PYHNEPGFEQ ERHPGDSKNY NECIRHETIR VAVCDMMEGK CPCPEPLRGV MEKSFLEYYD FYEVACKDRL HLQGQTMQDP FGEKRGHFDY QSLLMRLGLI RQKVLERLHN ENAEMDSDSS SSGTETDLHG SLRV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the covalent attachment of ubiquitin to other proteins. Specific substrate for UBA6, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation.
Gene References into Functions
  1. The SNP rs46522 in UBE2Z gene is associated with the risk of coronary Artery disease in the individuals of Chinese Han descent with type 2 diabetes and is of synergistic effect with Body mass index. PMID: 28840129
  2. we found that USE1 proteins were frequently overexpressed in human lung cancer. Furthermore, overexpression of USE1 promoted in vivo and in vitro proliferation, migration, and invasion. PMID: 28376205
  3. Association of UBE2Z single nucleotide polymorphism rs46522 with the susceptibility to coronary artery disease in an Iranian population. PMID: 26602883
  4. UBE2Z was widely expressed in human tissues and is located on chromosome 17q21. PMID: 17160626
  5. In tissue culture cells, Uba6 is required for charging a previously uncharacterized Uba6-specific E2 (Use1), whereas Ube1 is required for charging the cell-cycle E2s Cdc34A and Cdc34B PMID: 17597759

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Ubiquitin-conjugating enzyme family
Tissue Specificity
Widely expressed. Highly in placenta, pancreas, spleen and testis.
Database Links

HGNC: 25847

OMIM: 611362

KEGG: hsa:65264

STRING: 9606.ENSP00000354201

UniGene: Hs.514297

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