Recombinant Human Vesicle transport through interaction with t-SNAREs homolog 1B (VTI1B), partial

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Code CSB-EP887014HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
VTI1B
Uniprot No.
Research Area
Transport
Alternative Names
HGNC:17793; V SNARE; v-SNARE; Vesicle associated soluble NSF attachment protein receptor; Vesicle transport through interaction with t SNAREs 1B; Vesicle transport through interaction with t SNAREs homolog 1B (yeast); Vesicle transport through interaction with t-SNAREs homolog 1B; Vesicle transport v-SNARE protein Vti1-like 1; VTI1; VTI1 like; Vti1 rp1; VTI1-LIKE; Vti1-rp1; VTI1B; VTI1B_HUMAN; VTI1L; VTI1L1; VTI2
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
7-208aa
Target Protein Sequence
SSEHFEKLHEIFRGLHEDLQGVPERLLGTAGTEEKKKLIRDFDEKQQEANETLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSKSRKILRSMSRKVTTNKLL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
39.4kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.
Gene References into Functions
  1. Vti1b-dependent tethering of Lytic granule and CD3-endo determines accumulation, docking, and efficient lytic granule secretion at the immunological synapse. PMID: 21562157
  2. Results suggest that the combinational SNARE proteins VAMP8 and Vti1b mediate the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential event for autophagic degradation. PMID: 20089838
  3. A dual mechanism controlling the localization and function of exocytic v-SNAREs. PMID: 12853575
  4. Ca(2+) dissociates the Hrs-containing complex but not the VAMP-2-containing SNARE complex PMID: 14769786
  5. epsin 4 epsin-related protein is an adaptor for vti1b PMID: 15371541
  6. Results report that syntaxin 7, syntaxin 8, vti1b and VAMP8 physically and functionally interact with CFTR. PMID: 18570918

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Subcellular Location
Early endosome membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein. Lysosome membrane. Cytoplasmic granule. Recycling endosome membrane; Single-pass type IV membrane protein.
Protein Families
VTI1 family
Tissue Specificity
Expressed in all tissues examined.
Database Links

HGNC: 17793

OMIM: 603207

KEGG: hsa:10490

STRING: 9606.ENSP00000450731

UniGene: Hs.741177

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