Recombinant Human Vesicular glutamate transporter 3 (SLC17A8), partial

Code CSB-YP818771HU
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Source Yeast
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Code CSB-EP818771HU
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Source E.coli
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Code CSB-EP818771HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818771HU
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Source Baculovirus
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Code CSB-MP818771HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SLC17A8
Uniprot No.
Alternative Names
deafness autosomal dominant 25; DFNA 25; DFNA25; SLC17A8; Solute carrier family 17 (sodium dependent inorganic phosphate cotransporter) member 8; Solute carrier family 17 member 8; Vesicular glutamate transporter 3; VGLU3_HUMAN; VGLUT 3; VGluT3
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Gene References into Functions
  1. Novel pathogenic splicing mutation in SLC17A8 gene identified in a family with hearing loss. PMID: 28647561
  2. A point mutation in VGLUT3 dramatically reduces its expression in synaptic terminals without altering its function. PMID: 28314816
  3. The results of this study identify VGLUT3 as an unexpected regulator of drug abuse. PMID: 26239290
  4. The results from our study suggest that the p.M206Nfs*4 mutation in the SLC17A8 gene is likely a pathogenic mutation that causes autosomal dominant non-syndromic hearing loss. PMID: 26797701
  5. The absence of VGLUT3 in transgenic mice leads to an up-regulation of the midbrain dopamine system and ameliorates motor dysfunction in a model of Parkinson's disease. PMID: 26558771
  6. human vesicular glutamate transporter 3 was cloned, shows 72% identity to both hVGLUT1 and hVGLUT2, and its expression in brain overlaps with hVGLUT1 and hVGLUT2 PMID: 12151341
  7. Docking and homology modeling explain the inhibition of VGLUT3. PMID: 17660252
  8. VGLUT3 immunoreactivity covered only a limited portion of the possible Glu-ergic pathways in the human fetal small intestine. PMID: 18498073
  9. SLC17A8 encodes vesicular glutamate transporter-3, VGLUT3 and its impairment has a role in nonsyndromic deafness DFNA25 and inner hair cell dysfunction PMID: 18674745

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Involvement in disease
Deafness, autosomal dominant, 25 (DFNA25)
Subcellular Location
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
Protein Families
Major facilitator superfamily, Sodium/anion cotransporter family, VGLUT subfamily
Tissue Specificity
Expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord and thalamus.
Database Links

HGNC: 20151

OMIM: 605583

KEGG: hsa:246213

STRING: 9606.ENSP00000316909

UniGene: Hs.116871

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