Recombinant Human WD repeat and HMG-box DNA-binding protein 1 (WDHD1), partial

Code CSB-YP025994HU
MSDS
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Source Yeast
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Code CSB-EP025994HU
MSDS
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Source E.coli
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Code CSB-EP025994HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025994HU
MSDS
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Source Baculovirus
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Code CSB-MP025994HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WDHD1
Uniprot No.
Alternative Names
WDHD1; AND1; WD repeat and HMG-box DNA-binding protein 1; Acidic nucleoplasmic DNA-binding protein 1; And-1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication.
Gene References into Functions
  1. And-1 interacts with CtIP and that these interactions are required for DNA damage checkpoint maintenance, thereby linking DNA processing with prolonged cell cycle arrest to allow sufficient time for DNA repair. PMID: 27940552
  2. important role of And-1 in homologous recombination repair by regulating CtIP-mediated DNA double-strand break end resection PMID: 27940557
  3. findings provide important insights into the mechanistic details of human AND-1 function, advancing our understanding of replisome formation during eukaryotic replication. PMID: 28381552
  4. The WDHD1 gene, one of the genes that is upregulated in human papillomavirus E7-expressing cells, was found to play an important role in E7-induced G1 checkpoint abrogation and rereplication PMID: 27099318
  5. Results provide additional evidence suggesting that WDHD1 is involved in the mismatch repair pathway. PMID: 27037360
  6. RecQL4-dependent association of Mcm10 and Ctf4 with replication origins appears to be the first important step controlled by S phase promoting kinases and checkpoint pathways for the initiation of DNA replication in human cells. PMID: 25602958
  7. And-1 coordinates with Claspin for efficient Chk1 activation in response to replication stress. PMID: 26082189
  8. The Ctf4-CMG complex contains a homodimeric Ctf4 that acts as a platform linking polymerase alpha to the monomeric hCMG complex. PMID: 24255107
  9. And-1 together with HJURP regulates the assembly of new CENP-A onto centromeres. PMID: 23184928
  10. Human And-1 facilitates loading of the MCM2-7 helicase onto chromatin during the assembly of prereplicative complex. PMID: 23093411
  11. Data show that And-1 forms a complex with both histone H3 and Gcn5. PMID: 21725360
  12. in human cells, hCtf4 plays an essential role in DNA replication and its ability to stimulate the replicative DNA polymerases may contribute to this effect PMID: 20089864
  13. Mcm10 is required for chromatin loading of And-1. PMID: 17761813
  14. AND-1 coordinates multiple cellular events in S phase and G2 phase. PMID: 19439411
  15. Assembly of the Cdc45-Mcm2-7-GINS complex requires the Ctf4/And-1, RecQL4, and Mcm10 proteins. PMID: 19805216

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Subcellular Location
Nucleus, nucleoplasm.
Database Links

HGNC: 23170

OMIM: 608126

KEGG: hsa:11169

STRING: 9606.ENSP00000353793

UniGene: Hs.385998

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