Recombinant Human WD repeat domain-containing protein 83 (WDR83)

Code CSB-YP863110HU
MSDS
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Source Yeast
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Code CSB-EP863110HU
MSDS
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Source E.coli
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Code CSB-EP863110HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863110HU
MSDS
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Source Baculovirus
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Code CSB-MP863110HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WDR83
Uniprot No.
Alternative Names
MAPK organizer 1; MGC4238; Mitogen activated protein kinase organizer 1 ; Mitogen-activated protein kinase organizer 1; MORG 1; WD repeat domain-containing protein 83; wdr83; WDR83_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-315
Target Protein Sequence
MAFPEPKPRP PELPQKRLKT LDCGQGAVRA VRFNVDGNYC LTCGSDKTLK LWNPLRGTLL RTYSGHGYEV LDAAGSFDNS SLCSGGGDKA VVLWDVASGQ VVRKFRGHAG KVNTVQFNEE ATVILSGSID SSIRCWDCRS RRPEPVQTLD EARDGVSSVK VSDHEILAGS VDGRVRRYDL RMGQLFSDYV GSPITCTCFS RDGQCTLVSS LDSTLRLLDK DTGELLGEYK GHKNQEYKLD CCLSERDTHV VSCSEDGKVF FWDLVEGALA LALPVGSGVV QSLAYHPTEP CLLTAMGGSV QCWREEAYEA EDGAG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Molecular scaffold protein for various multimeric protein complexes. Acts as a module in the assembly of a multicomponent scaffold for the ERK pathway, linking ERK responses to specific agonists. At low concentrations it enhances ERK activation, whereas high concentrations lead to the inhibition of ERK activation. Also involved in response to hypoxia by acting as a negative regulator of HIF1A/HIF-1-alpha via its interaction with EGLN3/PHD3. May promote degradation of HIF1A. May act by recruiting signaling complexes to a specific upstream activator. May also be involved in pre-mRNA splicing.
Gene References into Functions
  1. Morg1 facilitates Par6-aPKC binding to Crb3 for definition of apical identity of epithelial cells. PMID: 23439680
  2. WDR83 and DHPS were capable of forming an RNA duplex at overlapping 3' untranslated regions and this duplex increased their mutual stability, which was required for the bidirectional regulation. PMID: 22491477
  3. Includes the identification of this protein in C complex spliceosomes. PMID: 11991638
  4. Functional characterization of the homologous rat gene. PMID: 16407229
  5. Component of a modular scaffold system that participates in the regulation of agonist-specific ERK signaling. PMID: 15118098
  6. Morg1 expression is reduced in human brain tissue with ischemic damage. Reactive astrocytes in the surrounding brain tissue showed strong Morg1 expression. PMID: 19429104

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
WD repeat MORG1 family
Database Links

HGNC: 32672

KEGG: hsa:84292

STRING: 9606.ENSP00000242796

UniGene: Hs.657204

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