Recombinant Human ZW10 interactor (ZWINT)

Code CSB-YP527261HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP527261HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP527261HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP527261HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ZWINT
Uniprot No.
Alternative Names
Human ZW10 interacting protein 1; HZwint 1; HZwint1; KNTC 2 AP; KNTC2AP; MGC 117174; MGC117174; ZW10 interacting kinetochore protein; ZW10 interacting protein 1; ZW10 interactor; ZW10 interactor; kinetochore protein; ZW10-interacting protein 1; ZWINT 1; ZWINT; Zwint-1; ZWINT_HUMAN; ZWINT1
Species
Homo sapiens (Human)
Expression Region
1-277
Target Protein Sequence
MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD KLLCSQLQVA DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK ATYREHVEAI KIGLTKALTQ MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG TQQELDRVFQ KLGNLKQQAE QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP QQPTRPQEQS TGDTMGRDPG VSFKAVGLQP AGDVNLP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
Gene References into Functions
  1. ZW10 interactor was frequently decreased in hepatocellular carcinoma. PMID: 30198401
  2. Our results provide the first evidence that Zwint-1 is required to correct erroneous kinetochore-microtubule attachment and regulate spindle checkpoint function during meiosis. PMID: 26486467
  3. the E3 ubiquitin ligase terf causes protein degradation of ZWINT and negatively regulates cell proliferation PMID: 22023800
  4. These studies identify zwint-1 as a novel AurB substrate required for kinetochore assembly and for proper spindle assembly checkpoint silencing at metaphase PMID: 21775627
  5. hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain PMID: 21345172
  6. show that ZW10 interacting protein-1(Zwint-1) is required and is sufficient for kinetochore localization of Zeste White 10 (ZW10) in HeLa cells PMID: 15485811
  7. Stable hZW10 kinetochore residency at prometaphase kinetochores is dependent on its interaction with hZwint-1, and is essential for mitotic checkpoint arrest. PMID: 18268100

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Subcellular Location
Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase.
Database Links

HGNC: 13195

OMIM: 609177

KEGG: hsa:11130

STRING: 9606.ENSP00000363055

UniGene: Hs.591363

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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