Recombinant Human Zinc finger protein Helios (IKZF2)

Code CSB-YP892138HU
MSDS
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Source Yeast
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Code CSB-EP892138HU
MSDS
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Source E.coli
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Code CSB-EP892138HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892138HU
MSDS
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Source Baculovirus
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Code CSB-MP892138HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IKZF2
Uniprot No.
Alternative Names
ANF1A2; HELIOS; IKAROS family zinc finger 2; Ikaros family zinc finger protein 2; IKZF 2; IKZF2; IKZF2_HUMAN; MGC34330; Zinc finger DNA binding protein Helios; Zinc finger protein Helios; Zinc finger protein subfamily 1A 2; ZNF1A2
Species
Homo sapiens (Human)
Expression Region
1-500
Target Protein Sequence
METEAIDGYITCDNELSPEREHSNMAIDLTSSTPNGQHASPSHMTSTNSVKLEMQSDEECDRKPLSREDEIRGHDEGSSLEEPLIESSEVADNRKVQELQGEGGIRLPNGERPFHCNQCGASFTQKGNLLRHIKLHSGEKPFKCPFCSYACRRRDALTGHLRTHSVGKPHKCNYCGRSYKQRSSLEEHKERCHNYLQNVSMEAAGQVMSHHVPPMEDCKEQEPIMDNNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Associates with Ikaros at centromeric heterochromatin.
Gene References into Functions
  1. The results suggested that increased methylation of Foxp3 Treg-specific demethylated region sequence and altered Helios gene expression in peripheral whole blood may have a role in the pathogenesis of rheumatoid arthritis via their effects on Regulatory T cell stability. PMID: 29851536
  2. Helios mRNA is down-regulated in Treg cells of patients with hypertension. PMID: 30074152
  3. mRNA expression of Helios was decreased in CD4+ T cells from patients with acute coronary syndrome. PMID: 29259350
  4. High expression of TIGIT and Helios identifies CD4+ T cells with impaired immunological functions, primarily among patients with an advanced stage of Sezary syndrome. PMID: 27592800
  5. The results show that high vs. low expression of Nrp-1 or Helios does not unequivocally identify Treg clones of thymic or peripheral origin. PMID: 26495986
  6. Helios could be considered a more reliable marker for distinguishing thymic derived Regulatory T (tTreg) cells or peripherally induced Treg cells than Nrp1. PMID: 25586548
  7. expression is not affected in diGeorge syndrome PMID: 25008482
  8. Forkhead box protein 3(+) regulatory T cells and Helios(+) subset in perinatally acquired HIV. PMID: 25425428
  9. Ectopic expression of ATL-type Helios isoform as well as knockdown of normal Helios or Ikaros promoted T-cell growth. PMID: 23600753
  10. Foxp3+ Helios+ regulatory T cells are expanded in active systemic lupus erythematosus. PMID: 23264341
  11. large number of ex vivo expanded functional Treg can be obtained from long-term T1D patients, although fewer expanded Treg expressed a high level of Helios PMID: 23409157
  12. A differential role for monocyte subsets in control of Helios(+/-) Treg development that is mediated by distinct inflammatory cytokines. PMID: 23365462
  13. Helios+ and Helios- cells coexist within the natural FOXP3+ T regulatory cell subset in humans. PMID: 23359504
  14. Presence of Helios-positive regulatory T (Treg) cells may maintain susceptibility to helminth infection on the C57BL/6 mouse background. PMID: 22753928
  15. Data found that Helios-negative T cells are enriched for naive T cell phenotypes and vice versa. Moreover, Helios can be induced during T cell activation and proliferation, but regresses in the same cells under resting conditions. PMID: 21918685
  16. Expanded peripheral FoxP3+ Tregs in untreated renal cell carcinoma patients co-express Helios. Interestingly, IL-2 administration results in expansion of FoxP3+Helios+ natural Tregs (nTregs) significantly more than FoxP3+Helios- iTregs. PMID: 21570917
  17. found that suppression of Helios message in CD4(+)CD25(+) T cells significantly attenuates their suppressive function. PMID: 20226531
  18. Novel short isoforms of Helios are overexpressed in a patient with T-cell acute lymphoblastic leukemia and may contribute to the development of T-cell malignancies. Data for unaltered Helios indicates tumor suppressor function. PMID: 11937265
  19. suggest a critical role for Helios in the mechanism of leukemogenesis PMID: 17297655

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Subcellular Location
Nucleus.
Protein Families
Ikaros C2H2-type zinc-finger protein family
Database Links

HGNC: 13177

OMIM: 606234

KEGG: hsa:22807

STRING: 9606.ENSP00000410447

UniGene: Hs.604950

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