Recombinant Human coronavirus NL63 Nucleoprotein (N)

Code CSB-YP744150HIX
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP744150HIX-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP744150HIX
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
N
Uniprot No.
Alternative Names
N; 6; Nucleoprotein; Nucleocapsid protein; NC; Protein N
Species
Human coronavirus NL63 (HCoV-NL63)
Expression Region
1-377
Target Protein Sequence
MASVNWADDR AARKKFPPPS FYMPLLVSSD KAPYRVIPRN LVPIGKGNKD EQIGYWNVQE RWRMRRGQRV DLPPKVHFYY LGTGPHKDLK FRQRSDGVVW VAKEGAKTVN TSLGNRKRNQ KPLEPKFSIA LPPELSVVEF EDRSNNSSRA SSRSSTRNNS RDSSRSTSRQ QSRTRSDSNQ SSSDLVAAVT LALKNLGFDN QSKSPSSSGT STPKKPNKPL SQPRADKPSQ LKKPRWKRVP TREENVIQCF GPRDFNHNMG DSDLVQNGVD AKGFPQLAEL IPNQAALFFD SEVSTDEVGD NVQITYTYKM LVAKDNKNLP KFIEQISAFT KPSSIKEMQS QSSHVAQNTV LNASIPESKP LADDDSAIIE IVNEVLH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Gene References into Functions
  1. solved the crystal structures of the N- and C-terminal domains (NTD, residues 10 to 140, and CTD, residues 221 to 340, respectively) of the N protein of HCoV-NL63, both at a 1.5-A resolution; investigated the structural arrangement of N protein, explaining the first steps of its interaction with nucleic acid at the initial stages of virus structure assembly PMID: 28331093
Subcellular Location
Virion. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus.
Protein Families
Alphacoronavirus nucleocapsid protein family
Database Links

KEGG: vg:2943504

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