Name: Recombinant Human immunodeficiency virus 1 Envelope glycoprotein gp160 (env), partial (Active)
Brand: CUSABIO
SKU: CSB-MP6761GJP
Price: 114 USD
Availability: InStock

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA.Immobilized HIV-1 env at 2 μg/mL can bind Human CD4(CSB-MP004935HU1).The EC50 is 12.28-19.67 ng/mL.

Target Names

env

Uniprot No.

ABL67444.1

Alternative Names

/

Source

Mammalian cell

Expression Region

36-507aa

Target Protein Sequence

YYGVPVWKEAKTTLFCASDAKAYEKEVHNVWATHACVPTDPNPQEMVLENVTENFNMWKNDMVEQMHEDVISLWDQSLKPCVKLTPLCVTLECRQVNTTNATSSVNVTNGEEIKNCSFNATTEIRDKKQKVYALFYRLDIVPLEEERKGNSSKYRLINCNTSAITQACPKVTFDPIPIHYCAPAGYAILKCNNKTFNGTGPCNNVSTVQCTHGIKPVVSTQLLLNGSLAEGEIIIRSENLTNNVKTIIVHLNESVEIVCTRPNNNTRKSIRIGPGQTFYATGDIIGNIRQAYCNIKKDDWIRTLQRVGKKLAEHFPRRIINFTSPAGGDLEITTHSFNCRGEFFYCNTSSLFNSTYNPNDTNSNSSSSNSSLDITIPCRIKQIINMWQEVGRAMYAPPIEGNITCKSNITGLLLVRDGGVESNETEIFRPGGGDMRNNWRSELYKYKVVEIKPLGIAPTAAKRRVVEREKRA

Mol. Weight

56.1 kDa

Protein Length

Partial

Tag Info

C-terminal Twin-Strep-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The Human immunodeficiency virus 1 Envelope glycoprotein gp160 (env) is a class I transmembrane glycoprotein that mediates viral attachment and fusion by engaging the CD4 receptor and CCR5/CXCR4 co-receptors, making it a central target for neutralizing antibody discovery and vaccine immunogen design. This recombinant HIV-1 gp160, partial (Active) (CSB-MP6761GJP) covers the immunodominant extracellular region (aa 36–507), is expressed in mammalian cells to preserve native trimeric conformation and glycosylation, and is engineered with a C-terminal Twin-Strep tag to facilitate high-purity purification. Functional ELISA confirms specific interaction with human CD4 (CSB-MP004935HU1) with an EC50 of 12.28–19.67 ng/mL, validating its structural integrity and receptor-binding capacity. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this preparation meets the rigorous standards required for neutralization assays, epitope mapping, and the evaluation of entry inhibitors in HIV-1 therapeutic research.

Code	CSB-MP6761GJP
Abbreviation	Recombinant Human immunodeficiency virus 1 env protein, partial (Active)
MSDS	MSDS Datasheet
Size	$114
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA.Immobilized HIV-1 env at 2 μg/ml can bind Human CD4(CSB-MP004935HU1).The EC₅₀ is 12.28-19.67 ng/mL. Biological Activity Assay
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Recombinant Human immunodeficiency virus 1 Envelope glycoprotein gp160 (env), partial (Active)

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