Recombinant Human immunodeficiency virus type 1 group M subtype K Protein Vpr (vpr)

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Code CSB-EP314796HLP
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
vpr
Uniprot No.
Research Area
Immunology
Alternative Names
vprProtein Vpr; R ORF protein; Viral protein R
Species
Human immunodeficiency virus type 1 group M subtype K (isolate 96CM-MP535) (HIV-1)
Source
E.coli
Expression Region
1-96aa
Target Protein Sequence
MEQAPEDQGPQREPNNEWTLEILEELKREAVRHFPRPWLHNLGQHIYTTYGDTWEGLEAIIRILQQLLFIHFRIGCHHSRIGIIPQRRGRNGSSRS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
18.8 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The expression region of this recombinant Human immunodeficiency virus type 1 vpr covers amino acids 1-96. The calculated molecular weight for this vpr protein is 18.8 kDa. This vpr recombinant protein is manufactured in e.coli. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of vpr, which enables a simple process of detecting and purifying the vpr recombinant protein in the following steps.

The human immunodeficiency virus type 1 (HIV-1) group M subtype K protein Vpr, or viral protein R, is a regulatory protein encoded by the vpr gene of the HIV-1 genome. Vpr plays a multifaceted role in the viral life cycle and pathogenesis. It is involved in nuclear import of the pre-integration complex, influencing viral transcription, and promoting the G2 cell cycle arrest of host cells, facilitating efficient viral replication. Additionally, Vpr contributes to immune evasion and modulation of host cell apoptosis. The protein's interaction with various host factors underscores its significance in HIV-1 replication and the progression of the infection. Research on HIV-1 Vpr is essential for understanding the molecular mechanisms of viral pathogenesis and can inform the development of targeted antiretroviral therapies.

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Target Background

Function
During virus replication, may deplete host UNG protein, and incude G2-M cell cycle arrest. Acts by targeting specific host proteins for degradation by the 26S proteasome, through association with the cellular CUL4A-DDB1 E3 ligase complex by direct interaction with host VPRPB/DCAF-1. Cell cycle arrest reportedly occurs within hours of infection and is not blocked by antiviral agents, suggesting that it is initiated by the VPR carried into the virion. Additionally, VPR induces apoptosis in a cell cycle dependent manner suggesting that these two effects are mechanistically linked. Detected in the serum and cerebrospinal fluid of AIDS patient, VPR may also induce cell death to bystander cells.; During virus entry, plays a role in the transport of the viral pre-integration (PIC) complex to the host nucleus. This function is crucial for viral infection of non-dividing macrophages. May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions.
Subcellular Location
Virion. Host nucleus. Host extracellular space.
Protein Families
HIV-1 VPR protein family
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