Recombinant Listeria monocytogenes serovar 1/2a Internalin-A (inlA), partial

Code CSB-YP338647LPY
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Source Yeast
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Code CSB-EP338647LPY-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP338647LPY
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Source Baculovirus
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Code CSB-MP338647LPY
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
inlA
Uniprot No.
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mediates the entry of L.monocytogenes into host intestinal epithelial cells; transformation with inlA alone allows L.innocua (a non-invasive species) to be taken up by host cells. Binds to human receptor cadherin-1 (E-cadherin, CDH1); the chicken homolog of cadherin-1 but not cadherin-2 function as receptors. Mouse cadherin-1 is not a receptor, however mutating a single surface-exposed residue (Glu-172 to Pro in mouse) allows cadherin-1 to act as a receptor for InlA.
Gene References into Functions
  1. Deletion of either one or both proteins leads to a similarly decreased invasion, suggesting an interdependent function of InlA and InlB during invasion of choroid plexus epithelial cells. PMID: 23376167
  2. About 40% of Listeria monocytogenes isolates (three subtypes) were found to potentially present attenuated virulence because of the presence of mutations in the prfA and inlA genes PMID: 23317868
  3. A novel single amino acid change in inlA enhanced virulence by the oral route in the murine model. PMID: 21144051
  4. Listeria monocytogenes internalin interacts with E-cadherin [review] PMID: 20066101
  5. L. monocytogenes subtypes are common among U.S. food isolates but rare among human listeriosis isolates carry inlA mutations. PMID: 16332872
  6. Differential expression levels of inlA and inlB possibly play a role in the virulence capacities of Listeria monocytogenes strains. PMID: 16751490
  7. Sequence analyses of inlA among L. monocytogenes isolated from ready-to-eat seafood revealed that only one out of 59 isolates had a nonsense-mutated inlA which indicated that these strains could be fully virulent based on the size of this protein. PMID: 17566579
  8. the essential and interdependent roles of InlA and InlB in fetoplacental listeriosis PMID: 18806773
  9. analysis of the internalin multigene family in Listeria PMID: 18840511
  10. truncated and/or proteolytically cleaved InlA are likely involved in the biofilm enhancement. PMID: 19309566

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Subcellular Location
Secreted, cell wall; Peptidoglycan-anchor. Secreted.
Database Links

KEGG: lmo:lmo0433

STRING: 169963.lmo0433

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