Recombinant Macaca fascicularis Interleukin-2 (IL2) (Active)

Interleukin-2 drives T-cell proliferation and immune activation through high-affinity receptor engagement, making quantitative binding data essential for interpreting dose-response experiments in primate immunology models. This recombinant cynomolgus IL-2, spanning the full mature sequence (aa 21–154), demonstrates receptor binding with an EC50 of 88.60–116.8 ng/mL in functional ELISA and a measured affinity constant of 31.3 nM by surface plasmon resonance against IL-2Rα, providing a validated basis for cell-based proliferation assays with primary cynomolgus PBMCs or T-cell lines and for JAK/STAT signaling pathway studies where precise cytokine potency is required. The protein meets the purity and endotoxin criteria typical for immune cell functional assays (>90% pure, <1.0 EU/μg), minimizing LPS-driven artifacts that can confound cytokine-specific responses in activation and differentiation experiments. Mammalian expression ensures native glycosylation, supporting use as a positive control in antibody validation workflows and as a reference standard in cynomolgus-specific cytokine detection platforms.